Calcium Sensing in Exocytosis

Gustavsson, Natalia; Wu, Bingbing; Han, Weiping

doi:10.1007/978-94-007-2888-2_32

Cited by 17 publications

(14 citation statements)

References 184 publications

(223 reference statements)

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“…5, Extended Data Fig. 5) [24][25][26] . This is in line with previous reports of neurosecretory cells populating the dorsomedian larval brain 22 , and with ultrastructural observations, which show that cells in the dorsomedian Platynereis brain are rich in dense core vesicles while synapses are often sparse or absent 27 .…”

Section: The Apical Nervous Systemmentioning

confidence: 99%

Whole-body single-cell sequencing of thePlatynereislarva reveals a subdivision into apical versus non-apical tissues

Achim¹,

Eling

H³

et al. 2017

Preprint

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(127 words)Animal bodies comprise a diverse array of tissues and cells. To characterise cellular identities across an entire body, we have compared the transcriptomes of single cells randomly picked from dissociated whole larvae of the marine annelid Platynereis dumerilii 1-4 . We identify five transcriptionally distinct groups of differentiated cells that are spatially coherent, as revealed by spatial mapping 5 . Besides somatic musculature, ciliary bands and midgut, we find a group of cells located at the apical tip of the animal, comprising sensory-peptidergic neurons, and another group composed of non-apical neural and epidermal cells covering the rest of the body. These data establish a basic subdivision of the larval body surface into molecularly defined apical versus non-apical tissues, and support the evolutionary conservation of the apical nervous system as a distinct part of the bilaterian brain 6 .

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Section: The Apical Nervous Systemmentioning

confidence: 99%

Whole-body single-cell sequencing of thePlatynereislarva reveals a subdivision into apical versus non-apical tissues

Achim¹,

Eling

H³

et al. 2017

Preprint

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“…Oxidized arachidonic acid metabolites, such as 5-oxo-ETE or leukotriene B4, are powerful inflammatory signals that mediate rapid leukocyte recruitment to zebrafish tail fin wounds and dermal injury sites in the mouse ear, respectively [25,28]. In addition, Ca 2+ governs the secretion of paracrine mediators via exocytosis and/or plasma membrane channels [106,107]. Aside from paracrine wound signaling, Ca 2+ is also required for epithelial sheet motility and integrity.…”

Section: Figurementioning

confidence: 99%

Mechanisms of epithelial wound detection

Enyedi¹,

Niethammer²

2015

Trends in Cell Biology

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Efficient wound healing requires the coordinated responses of various cell types within an injured tissue. To react to the presence of a wound, cells have to first detect it. Judging from their initial biochemical and morphological responses, many cells including leukocytes, epithelial and endothelial cells, detect wounds from over hundreds of micrometers within seconds-to-minutes. Wound detection involves the conversion of an injury-induced homeostatic perturbation, such as cell lysis, an unconstrained epithelial edge, or permeability barrier breakdown, into a chemical or physical signal. The signal is spatially propagated through the tissue to synchronize protective responses of cells near the wound site and at a distance. This review summarizes the triggers and mechanisms of wound detection in animals.

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“…The production and secretion of hormones by the pituitary is deregulated in tumor development (21). Regulated exocytosis is triggered by increased intracellular calcium levels (22), and vesicle trafficking and secretion are regulated by a core fusion machinery consisting of SNARE proteins (soluble N-ethylmaleimidesensitive factor attachment protein receptor proteins). ESRPs have been shown to have targets among transcripts in vesicle-mediated protein trafficking, and the exocyst has essential functions in the maintenance of cell polarity and cell-cell adhesion (12,23).…”

Section: Discussionmentioning

confidence: 99%

Epithelial Splicing Regulator Protein 1 and Alternative Splicing in Somatotroph Adenomas

et al. 2013

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Somatotroph adenomas secrete supraphysiological amounts of GH, causing acromegaly. We have previously hypothesized that epithelial mesenchymal transition (EMT) may play a central role in the progression of these adenomas and that epithelial splicing regulator 1 (ESRP1) may function prominently as a master regulator of the EMT process in pituitary adenomas causing acromegaly. To further elucidate the role of ESRP1 in somatotroph adenomas and in EMT progression, we used RNA sequencing (RNAseq) to sequence somatotroph adenomas characterized by high and low ESRP1 levels. Transcripts identified by RNAseq were analyzed in 65 somatotroph adenomas and in GH-producing pituitary rat cells with a specific knockdown of Esrp1. The clinical importance of the transcripts was further investigated by correlating mRNA expression levels with clinical indices of disease activity and treatment response. Many of the transcripts and isoforms identified by RNAseq and verified by quantitative PCR were involved in vesicle transport and calcium signaling and were associated with clinical outcomes. Silencing Esrp1 in GH3 cells resulted in changes of gene expression overlapping the data observed in human somatotroph adenomas and revealed a decreased granulation pattern and attenuated GH release. We observed an alternative splicing pattern for F-box and leucine-rich repeat protein 20, depending on the ESPR1 levels and on changes in circulating IGF-I levels after somatostatin analog treatment. Our study indicates that ESRP1 in somatotroph adenomas regulates transcripts that may be essential in the EMT progression and in the response to somatostatin analog treatment.

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Calcium Sensing in Exocytosis

Cited by 17 publications

References 184 publications

Whole-body single-cell sequencing of thePlatynereislarva reveals a subdivision into apical versus non-apical tissues

Whole-body single-cell sequencing of thePlatynereislarva reveals a subdivision into apical versus non-apical tissues

Mechanisms of epithelial wound detection

Epithelial Splicing Regulator Protein 1 and Alternative Splicing in Somatotroph Adenomas

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