Calibrating 10-AU fluorometer to quantify extracted chl-a v1

Abstract

This method is for use for calibrating the 10-AU flurometer using chl-a extracts. The method also provides guidelines for extracting chl-a and calculating absorbance using a spectrophotometer, making dilutions of extracts that can be used to create differetn chl-a concentrations THIS PROTOCOL ACCOMPANIES THE FOLLOWING PUBLICATION S.W. Jeffrey, G.F. Humphrey, New spectrophotometric equations for determining chlorophylls a, b, c1 and c2 in higher plants, algae and natural phytoplankton, Biochemie und Physiologie der Pflanzen, Volume 167, Issue 2, 1975, Pages 191-194,