Calibration Curve Fitting

Dunn, John R.; Wild, David

doi:10.1016/b978-0-08-097037-0.00022-1

Cited by 18 publications

(19 citation statements)

References 19 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…To calculate cytokine sample concentrations from the standard curves, we used a four-parameter logistic regression model 36,37 implemented in R with the package minpack . lm .…”

Section: Methodsmentioning

confidence: 99%

Cytokine response following perturbation of the cervicovaginal milieu during HPV genital infection

Selinger

Rahmoun

Murall

et al. 2021

Preprint

View full text Add to dashboard Cite

Human papillomaviruses (HPVs) are the most oncogenic viruses known to human, causing nearly all cervical cancers worldwide. Highly prevalent in young, sexually active women, most HPV infections are cleared within 3 years, and only a minority of those infections persist and lead to cancer later in life. To better characterize the immuno-modulatory impact of early HPV infection and more generally perturbations of the cervicovaginal milieu, we measured changes in a panel of 20 cytokines, known as highly dynamic effector molecules implicated in cell signaling. We analyzed 92 cervicovaginal samples collected from young, sexually active women who were tested for or diagnosed with HPV, chlamydia, and bacterial vaginosis. Also, symptoms associated with genital inflammation and infection were collected through self-reporting. Following a parsimonious multi-factor modeling approach, our statistical analyses revealed that increased IL-1Alpha and IL-12/IL-23p40 concentrations were associated with HPV infection. Cytokine network analysis further highlighted the role of IL-1Alpha and macrophage inflammatory proteins (MIP-3Alpha) in HPV-associated immuno-modulation.

show abstract

“…To calculate cytokine sample concentrations from the standard curves, we used a four-parameter logistic regression model 36,37 implemented in R with the package minpack . lm .…”

Section: Methodsmentioning

confidence: 99%

Cytokine response following perturbation of the cervicovaginal milieu during HPV genital infection

Selinger

Rahmoun

Murall

et al. 2021

Preprint

View full text Add to dashboard Cite

show abstract

“…The dose-response curves were adjusted using the 4-parameter logistic model according to the following formula: y = A2 + (A1 − A2)/(1 + (x/x 0 )^p), with the parameters A1 the minimum initial value, A2 the maximum final value, x 0 the center and the power p = 3 of the sigmoidal curve. To determine the corresponding values the iteration algorithm according to Levenberg–Marquardt [41] was used. The median effective concentration (EC 50 = x 0 ) was defined as the concentration at which the immunoglobulins exerts half of their maximal optical density(OD).…”

Section: Methodsmentioning

confidence: 99%

Treatment and Prevention of Recurrent Clostridium difficile Infection with Functionalized Bovine Antibody-Enriched Whey in a Hamster Primary Infection Model

Heidebrecht

Weiss

Pulse

et al. 2019

Toxins

View full text Add to dashboard Cite

Toxin-induced Clostridium difficile infection (CDI) is a major disease characterized by severe diarrhea and high morbidity rates. The aim with this study was to develop an alternative drug for the treatment of CDI. Cows were repeatedly immunized to establish specific immunoglobulin G and A titers against toxins A (TcdA) and B (TcdB) and against C. difficile cells in mature milk or colostrum. The effect of three different concentrations of anti-C. difficile whey protein isolates (anti-CD-WPI) and the standard of care antibiotic vancomycin were investigated in an animal model of CD infected hamsters (6 groups, with 10 hamsters each). WPI obtained from the milk of exactly the same cows pre-immunization and a vehicle group served as negative controls. The survival of hamsters receiving anti-CD-WPI was 50, 80 and 100% compared to 10 and 0% for the control groups, respectively. Vancomycin suppressed the growth of C. difficile and thus protected the hamsters at the time of administration, but 90% of these hamsters nevertheless died shortly after discontinuation of treatment. In contrast, the surviving hamsters of the anti-CD-WPI groups survived the entire study period, although they were treated for only 75 h. The specific antibodies not only inactivated the toxins for initial suppression of CDI, but also provoked the inhibition of C. difficile growth after discontinuation, thus preventing recurrence. Oral administration of anti-CD-WPI is a functional therapy of CDI in infected hamsters for both primary treatment and prevention of recurrence. Thus, anti-CD-WPI could address the urgent unmet medical need for treating and preventing recurrent CDI in humans.

show abstract

“…MRZH reaction proficiency survey samples of the October 2012 to April 2016 campaigns were reassessed with algorithms based on modifications of two methods: the α method 8 and the 4PL method. 6,7 In a first step, the shortcomings of the single-pair evaluation were identified. The second step went into the development of methods for multiple-point evaluation.…”

Section: Introductionmentioning

confidence: 99%

“…This procedure is similar to setting up a calibration curve. 6,7 However, there are crucial differences: First, OD values from patient samples are analyzed instead of standardized samples. Second, not only method parameters are fitted to the data, but also concentration parameters (or their ratio).…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Multiple-Point Evaluation Algorithms for Enhanced Precision of Pathogen-Specific Cerebrospinal Fluid/Serum Antibody Index Calculation

et al. 2019

View full text Add to dashboard Cite

Calibration Curve Fitting

Cited by 18 publications

References 19 publications

Cytokine response following perturbation of the cervicovaginal milieu during HPV genital infection

Cytokine response following perturbation of the cervicovaginal milieu during HPV genital infection

Treatment and Prevention of Recurrent Clostridium difficile Infection with Functionalized Bovine Antibody-Enriched Whey in a Hamster Primary Infection Model

Multiple-Point Evaluation Algorithms for Enhanced Precision of Pathogen-Specific Cerebrospinal Fluid/Serum Antibody Index Calculation

Contact Info

Product

Resources

About