Callus induction, shoot proliferation and root regeneration of potato (Solanum tuberosumL.) stem node and leaf explants under long-day conditions

Kumlay, Ahmet Metin; Erċışlı, Sezai

doi:10.1080/13102818.2015.1077685

Cited by 58 publications

(37 citation statements)

References 14 publications

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“…It means that the root length of plantlet is influenced by the number of roots. It is in line with the results of the previous study on in vitro root regeneration of potatoes (Kumlay and Ercisli, 2015) that the root numbers of the plantlets were followed by the increase in root length. Root formation is a critical step.…”

Section: Regression Of Observed Variablessupporting

confidence: 91%

Modification of Media for Banana In Vitro Propagation with Foliar Fertilizer and Coconut Water in cv. Rajabulu

Mardhikasari

Yunus

Samanhudi

2019

Caraka Tani J. Sustain. Agric.

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The use of foliar fertilizers and coconut water is potential; foliar fertilizer is the alternative medium and coconut water is the plant growth regulator (PGR) in the banana tissue culture. This study aimed to examine the ability of foliar fertilizer to substitute Murashige and Skoog (MS) media and coconut water as the main source of cytokine. There are two factors in this research. The first factor is the media, which consist of three levels, i.e. fully MS, ½ MS + ½ foliar fertilizer and fully foliar fertilizer. The second factor is the coconut water with four levels of concentration i.e. 50, 100, 150 and 200 ml l-1. The results show that fully formulated foliar fertilizer had not been able to substitute or even compete with a fully MS media, but conversely foliar fertilizer was used to substitute ½ MS media. This can be seen from the parameters of shoot length, leaf length, root number and root length with the highest yield that was found in MS media (8.7, 4.66, 3.33 and 3.23 cm). The 50-100 ml l-1 concentrations of coconut water showed the best results by giving a significant effect on the number of roots and root length. The acclimatization of plantlets in this study was nearly 100%, indicated by the formation of a complete organ.

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Section: Regression Of Observed Variablessupporting

confidence: 91%

Modification of Media for Banana In Vitro Propagation with Foliar Fertilizer and Coconut Water in cv. Rajabulu

Mardhikasari

Yunus

Samanhudi

2019

Caraka Tani J. Sustain. Agric.

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“…For stress treatments, randomly selected uniform plantlets of each cultivar were transferred to MS medium (control) and MS with addition of 20 mM LiCl (LiCl treatment) [38] or 150 mM mannitol (mannitol treatment) [95]. Plant growth regulators BAP 2 mgL −1 , IBA 1 mgL −1 , and 0.25 mgL −1 GA 3 [96] were supplemented in all media. Plantlets were grown for 45 days before harvest and further analysis.…”

Section: In Vitro Potato Plantlets Growth and Treatment With Licl Andmentioning

confidence: 99%

Metabolomic and Biochemical Analysis of Two Potato (Solanum tuberosum L.) Cultivars Exposed to In Vitro Osmotic and Salt Stresses

Hamooh

Sattar

Wellman

et al. 2021

Plants

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Globally, many crop production areas are threatened by drought and salinity. Potato (Solanum tuberosum L.) is susceptible to these challenging environmental conditions. In this study, an in vitro approach was employed to compare the tolerance of potato cultivars ‘BARI-401’ (red skin) and ‘Spunta’ (yellow skin). To simulate ionic and osmotic stress, MS media was supplemented with lithium chloride (LiCl 20 mM) and mannitol (150 mM). GC-MS and spectrophotometry techniques were used to determine metabolite accumulation. Other biochemical properties, such as total phenols concentration (TPC), total flavonoids concentration (TFC), antioxidant capacity (DPPH free radical scavenging capacity), polyphenol oxidase (PPO), and peroxidase (POD) activities, were also measured. The two cultivars respond differently to ionic and osmotic stress treatments, with Spunta accumulating more defensive metabolites in response, indicating a higher level of tolerance. While further investigation of the physiological and biochemical responses of these varieties to drought and salinity is required, the approach taken in this paper provides useful information prior to open field evaluation.

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“…The in vitro propagation technique has been proven to be one of the most powerful tools to mass produce and accelerate production of medicinal plants, food crops, and ornamental plants [ 8 ]. In addition, this approach can be a way for a large-scale production of seedlings or calluses to support commercial industries such as herbal, nutraceutical, and pharmaceutical industries [ 9 ].…”

Section: Introductionmentioning

confidence: 99%

Induction, Multiplication, and Evaluation of Antioxidant Activity of Polyalthia bullata Callus, a Woody Medicinal Plant

Zaman

Azzeme

Ramle

et al. 2020

Plants

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Polyalthia bullata is an endangered medicinal plant species. Hence, establishment of P. bullata callus culture is hoped to assist in mass production of secondary metabolites. Leaf and midrib were explants for callus induction. Both of them were cultured on Murashige and Skoog (MS) and Woody Plant Medium (WPM) containing different types and concentrations of auxins (2,4-dichlorophenoxyacetic acid (2,4-D), α-naphthaleneacetic acid (NAA), picloram, and dicamba). The callus produced was further multiplied on MS and WPM supplemented with different concentrations of 2,4-D, NAA, picloram, dicamba, indole-3-acetic acid (IAA), and indole-3-butyric acid (IBA) media. The quantification of total phenolic content (TPC), total flavonoid content (TFC) and antioxidant capacity was further carried out on P. bullata callus, and the results were subjected to correlation analysis. Among the media, the WPM + 16.56 µM picloram (53.33 ± 22.06%) was the best for callus induction while MS + 30 µM dicamba was the best for callus multiplication. The TPC, TFC, and EC50 of DPPH scavenging activity were determined at 0.657 ± 0.07 mg GAE/g FW, 0.491 ± 0.03 mg QE/g, and 85.59 ± 6.09 µg/mL in P. bullata callus, respectively. The positive correlation between DPPH scavenging activity with TPC was determined at r = 0.869, and that of TFC was at r = 0.904. Hence, the P. bullata callus has an ability to accumulate antioxidants. It therefore can be a medium for secondary metabolites production.

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Callus induction, shoot proliferation and root regeneration of potato (Solanum tuberosumL.) stem node and leaf explants under long-day conditions

Cited by 58 publications

References 14 publications

Modification of Media for Banana In Vitro Propagation with Foliar Fertilizer and Coconut Water in cv. Rajabulu

Modification of Media for Banana In Vitro Propagation with Foliar Fertilizer and Coconut Water in cv. Rajabulu

Metabolomic and Biochemical Analysis of Two Potato (Solanum tuberosum L.) Cultivars Exposed to In Vitro Osmotic and Salt Stresses

Induction, Multiplication, and Evaluation of Antioxidant Activity of Polyalthia bullata Callus, a Woody Medicinal Plant

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