CaM and CML emergence in the green lineage

Zhu, Xiaoyang; Dunand, Christophe; Snedden, Wayne A.; Galaud, Jean‐Philippe

doi:10.1016/j.tplants.2015.05.010

Cited by 148 publications

(141 citation statements)

References 66 publications

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“…Calcium, especially cytosolic free Ca 2+ , has been widely recognized as a key signal molecule in plants and is involved in multiple signal transduction pathways, which mediate plant adaptive responses to abiotic and biotic stimuli [9]. The involvement of calcium in hypoxia responses has been observed in many plants such as rice, wheat, and cucumber [10, 11].…”

Section: Introductionmentioning

confidence: 99%

The effect of exogenous calcium on cucumber fruit quality, photosynthesis, chlorophyll fluorescence, and fast chlorophyll fluorescence during the fruiting period under hypoxic stress

et al. 2018

BMC Plant Biol

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BackgroundPlants often suffer from hypoxic stress during waterlogging and hydroponic culturing. This study investigated the response of cucumber (Cucumis sativus L.) plant growth parameters, leaf photosynthesis, chlorophyll fluorescence, fast chlorophyll a fluorescence transient (OJIP), and fruit quality parameters to hypoxic stress alleviated by exogenous calcium. During the fruiting period, cucumber plants were exposed to hypoxia and hypoxia + Ca2+ treatment (4 mM Ca2+) for 9 d.ResultExogenous calcium application enhanced the biomass and fruit quality of hypoxic stressed cucumber and also increased the net photosynthesis rate, stomatal conductance, intercellular CO2 concentration, maximum quantum efficiency of photosystem II photochemistry, actual photochemical efficiency of PSII, photochemical quenching coefficient, and non-photochemical quenching coefficient. Additionally, measurement of chlorophyll a fluorescence transients showed the positive K- and L-bands were more pronounced in leaves treated with hypoxia compared with those with hypoxia + Ca2+, indicating that hypoxic treatment induced uncoupling of the oxygen-evolving complex and inhibited electron transport beyond plastoquinone pool (Qa, Qb) including possible constraints on the reduction of end electron acceptors of photosystem I. Exogenous calcium can reduce these stress-induced damages in cucumber.ConclusionThis research focused the effect of exogenous calcium on cucumber photosynthesis during the fruiting period under hypoxic stress. Hypoxic stress might impair the photosynthetic electron-transport chain from the donor side of PSII up to the reduction of end acceptors of PSI, and exogenous calcium enhanced electron transport capacity and reduced hypoxic damage of cucumber leaves.

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Section: Introductionmentioning

confidence: 99%

The effect of exogenous calcium on cucumber fruit quality, photosynthesis, chlorophyll fluorescence, and fast chlorophyll fluorescence during the fruiting period under hypoxic stress

et al. 2018

BMC Plant Biol

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“…However, plant genomes do not encode IQGAPs, and the mechanisms of Ca 2+ -mediated cytoskeletal organization are largely elusive (Hepler, 1992(Hepler, , 2005Wang et al, 2011). During the colonization of terrestrial habitats, novel adaptive traits evolved in land plants (Graham, 1996), and the size of plant CaM/CML families expanded greatly to provide versatility for transducing complex Ca 2+ signals into numerous cellular and environmental responses (McCormack et al, 2005;Zhu et al, 2015b). Likewise, the cytoskeleton acquired additional functions for adapting cell shape and cell growth, and a multitude of unique MAPs emerged for generating the various cytoskeletal arrays and controlling their organization and stability (Gardiner, 2013;Struk and Dhonukshe, 2014).…”

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confidence: 99%

The IQD Family of Calmodulin-Binding Proteins Links Calcium Signaling to Microtubules, Membrane Subdomains, and the Nucleus

et al. 2017

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ORCID IDs: 0000-0002-3493-4800 (K.B.); 0000-0002-7146-043X (B.M.).Calcium (Ca 2+ ) signaling and dynamic reorganization of the cytoskeleton are essential processes for the coordination and control of plant cell shape and cell growth. Calmodulin (CaM) and closely related calmodulin-like (CML) polypeptides are principal sensors of Ca 2+ signals. CaM/CMLs decode and relay information encrypted by the second messenger via differential interactions with a wide spectrum of targets to modulate their diverse biochemical activities. The plant-specific IQ67 DOMAIN (IQD) family emerged as possibly the largest class of CaM-interacting proteins with undefined molecular functions and biological roles. Here, we show that the 33 members of the IQD family in Arabidopsis (Arabidopsis thaliana) differentially localize, using green fluorescent protein (GFP)-tagged proteins, to multiple and distinct subcellular sites, including microtubule (MT) arrays, plasma membrane subdomains, and nuclear compartments. Intriguingly, the various IQD-specific localization patterns coincide with the subcellular patterns of IQD-dependent recruitment of CaM, suggesting that the diverse IQD members sequester Ca 2+ -CaM signaling modules to specific subcellular sites for precise regulation of Ca 2+ -dependent processes. Because MT localization is a hallmark of most IQD family members, we quantitatively analyzed GFP-labeled MT arrays in Nicotiana benthamiana cells transiently expressing GFP-IQD fusions and observed IQD-specific MT patterns, which point to a role of IQDs in MT organization and dynamics. Indeed, stable overexpression of select IQD proteins in Arabidopsis altered cellular MT orientation, cell shape, and organ morphology. Because IQDs share biochemical properties with scaffold proteins, we propose that IQD families provide an assortment of platform proteins for integrating CaM-dependent Ca 2+ signaling at multiple cellular sites to regulate cell function, shape, and growth.

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“…8 The results show that the abundance of CaMs and CMLs evolved during terrestrial colonization of plants and that the emergence of new CML classes appear throughout the green lineage that correlate with land colonization by plants and with the acquisition of novel biologic traits. We can then speculate that each CMLs may be associated to specific functions during plant development and stress response as it was the case for CML8 and CML9.…”

Section: Discussionmentioning

confidence: 85%

“…7 These Ca 2C sensors are classified into 4 major groups, the calcineurin-B like, the calciumdependent kinases, the calmodulin group and a closely related group, the CalModulin-Like protein (CML) family, which is specific to plants. 8 In 2017, we reported that the ectopic CML8 expression (P35S:: cds CML8) confers to transgenic plants a better resistance to a virulent phytopathogenic bacteria (P. syringae pv tomato, Pst) compared with wild type (Col8) or the cml8 knock-down and knockout lines. 9 Collectively, our results support the idea that CML8 acts as a positive regulator of plant immunity.…”

Section: Introductionmentioning

confidence: 99%

Respective contribution of CML8 and CML9, two arabidopsis calmodulin-like proteins, to plant stress responses

Zhu

Perez

Aldon

et al. 2017

Plant Signaling & Behavior

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In their natural environment, plants have to continuously face constraints such as biotic and abiotic stresses. To achieve their life cycle, plants have to perceive and interpret the nature, but also the strength of environmental stimuli to activate appropriate physiological responses. Nowadays, it is well established that signaling pathways are crucial steps in the implementation of rapid and efficient plant responses such as genetic reprogramming. It is also reported that rapid raises in calcium (Ca 2C ) levels within plant cells participate in these early signaling steps and are essential to coordinate adaptive responses. However, to be informative, calcium increases need to be decoded and relayed by calcium-binding proteins also referred as calcium sensors to carry-out the appropriate responses. In a recent study, we showed that CML8, an Arabidopsis calcium sensor belonging to the calmodulin-like (CML) protein family, promotes plant immunity against the phytopathogenic bacteria Pseudomonas syringae pv tomato (strain DC3000). Interestingly, other CML proteins such as CML9 were also reported to contribute to plant immunity using the same pathosystem. In this addendum, we propose to discuss about the specific contribution of these 2 CMLs in stress responses. KEYWORDSArabidopsis thaliana; biotic and abiotic stress responses; calmodulin-like protein; calcium sensor; pseudomonas syringaeIt is now well documented that most of external biotic and abiotic stimuli induce a rapid increase in free calcium levels within plant cells. 1 Calcium is considered as an ubiquitous and versatile second messenger. Indeed, calcium variations are at the heart of sophisticated signaling networks, integrating information from a diverse range of developmental cues and environmental challenges that ultimately will impact gene expression and cell physiology. 2 Interestingly, these variations can harbour stimulusspecific properties 3 and according to the nature and strength of the stimuli, calcium variations display differences in frequencies, amplitudes, cellular localization and they were defined as «Ca 2C signature». 4 These Ca 2C signatures have been proven to encode a first layer of response specificity through their spatio-temporal properties. 3,5 A second layer of specificity is brought by the decoding processes requiring calcium-binding proteins also referred as calcium sensors and their downstream targets. Ca 2C sensors are characterized by the presence in their sequence of the canonical Ca 2C -binding motif called EF-hand. 6 The Arabidopsis genome encodes at least 250 EF-hand proteins, which is much higher than in mammals, arguing for higher importance of this class of proteins in plants. 7 These Ca 2C sensors are classified into 4 major groups, the calcineurin-B like, the calciumdependent kinases, the calmodulin group and a closely related group, the CalModulin-Like protein (CML) family, which is specific to plants. 8 In 2017, we reported that the ectopic CML8 expression (P35S:: cds CML8) confers to transgenic plants a ...

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CaM and CML emergence in the green lineage

Cited by 148 publications

References 66 publications

The effect of exogenous calcium on cucumber fruit quality, photosynthesis, chlorophyll fluorescence, and fast chlorophyll fluorescence during the fruiting period under hypoxic stress

The effect of exogenous calcium on cucumber fruit quality, photosynthesis, chlorophyll fluorescence, and fast chlorophyll fluorescence during the fruiting period under hypoxic stress

The IQD Family of Calmodulin-Binding Proteins Links Calcium Signaling to Microtubules, Membrane Subdomains, and the Nucleus

Respective contribution of CML8 and CML9, two arabidopsis calmodulin-like proteins, to plant stress responses

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