Can ecological history influence response to pollutants? Transcriptomic analysis of Manila clam collected in different Venice lagoon areas and exposed to heavy metal

Milan, Massimo; Matozzo, Valerio; Pauletto, Marianna; Camillo, Barbara Di; Giacomazzo, Matteo; Boffo, Luciano; Binato, Giovanni; Marin, María Gabriella; Patarnello, Tomaso; Bargelloni, Luca

doi:10.1016/j.aquatox.2016.02.024

Cited by 28 publications

(25 citation statements)

References 69 publications

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“…In this study, the combination of transcriptomic and microbiota profiles and the documented high contamination load in river run‐off suggest a different scenario, whereby xenobiotic exposure may instead play a crucial role in clam mortality. Three reasons may explain the discrepancy between this study and the one by Milan, Matozzo, et al () and Milan, Palazzo, et al (). First, lack of transcriptional differences between T4 and T7 related to xenobiotics response in Milan, Matozzo, et al () and Milan, Palazzo, et al () might be ascribed to geographical proximity of the two sites, considered to be equally impacted by chemical stress (Figure ).…”

Section: Discussioncontrasting

confidence: 97%

“…Three reasons may explain the discrepancy between this study and the one by Milan, Matozzo, et al () and Milan, Palazzo, et al (). First, lack of transcriptional differences between T4 and T7 related to xenobiotics response in Milan, Matozzo, et al () and Milan, Palazzo, et al () might be ascribed to geographical proximity of the two sites, considered to be equally impacted by chemical stress (Figure ). In this study, we introduced a new control site (Cupra Grottamare; CG) located north to the Pescara river mouth and therefore less affected by the river plume and discharge.…”

Section: Discussioncontrasting

confidence: 97%

“…In our previous study, gene expression profiles of the striped venus C. gallina in a mortality site (T4) revealed the up‐regulation of several genes involved in immune response. The results obtained suggested bacterial and/or viral infection as the major cause of clam mortality (Milan, Matozzo, et al, ; Milan, Palazzo, et al, ). Here, by using a combination of microbiota characterization (16S) and host‐gene expression profile through next‐generation sequencing (NGS), we provide a new hypothesis on the mechanism that may have impaired the physiological response of clams and favoured infection.…”

Section: Discussionmentioning

confidence: 99%

“…Indirect evidence of potential exposure to chemical pollutants is provided by C. gallina gene expression profiles, which show a large number of up‐regulated genes involved in xenobiotic metabolism and in the antioxidant defense system in sites T4 and T7 when compared to CG, such as CYP450 (Livingstone, Kirchin, & Wiseman, ; Stegeman, ; Zanette, Goldstone, Bainy, & Stegeman, ), SULT (Milan, Ferraresso, et al, ; Milan, Matozzo, et al, ; Milan, Palazzo, et al, ; Pessatti et al, ), GSTs (Mezzelani et al, ; Milan, Matozzo, et al, ; Milan et al, ; Park, Ahn, Kim, Lee, & Shin, ; Xu, Pan, Liu, Wang, & Miao, ) and GPX (Cossu et al, ). Noteworthy, GADD45G, that is involved in DNA repair, cell cycle control and apoptosis (Fornace, Jackman, Hollander, Hoffman‐Liebermann, & Liebermann, ), showed the highest fold changes in mortality sites when compared to the control site.…”

Section: Discussionmentioning

confidence: 99%

“…In a previous study by Milan, Matozzo, et al () and Milan, Palazzo, et al (), chemical pollution was ruled out as a primary trigger of clam mortality based on the chemical analyses and gene expression profiles of C. gallina in sites T4 and T7. In this study, the combination of transcriptomic and microbiota profiles and the documented high contamination load in river run‐off suggest a different scenario, whereby xenobiotic exposure may instead play a crucial role in clam mortality.…”

Section: Discussionmentioning

confidence: 99%

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Host‐microbiota interactions shed light on mortality events in the striped venus clam Chamelea gallina

et al. 2019

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Mass mortalities due to disease outbreaks have recently affected a number of major taxa in marine ecosystems. Climate‐ and pollution‐induced stress may compromise host immune defenses, increasing the risk of opportunistic diseases. Despite growing evidence that mass mortality events affecting marine species worldwide are strongly influenced by the interplay of numerous environmental factors, the reductionist approaches most frequently used to investigate these factors hindered the interpretation of these multifactorial pathologies. In this study, we propose a broader approach based on the combination of RNA‐sequencing and 16S microbiota analyses to decipher the factors underlying mass mortality in the striped venus clam, Chamelea gallina, along the Adriatic coast. On one hand, gene expression profiling and functional analyses of microbial communities showed the over‐expression of several genes and molecular pathways involved in xenobiotic metabolism, suggesting potential chemical contamination in mortality sites. On the other hand, the down‐regulation of several genes involved in immune and stress response, and the over‐representation of opportunistic pathogens such as Vibrio and Photobacterium spp. indicates that these microbial species may take advantage of compromised host immune pathways and defense mechanisms that are potentially affected by chemical exposure, resulting in periodic mortality events. We propose the application of our approach to interpret and anticipate the risks inherent in the combined effects of pollutants and microbes on marine animals in today's rapidly changing environment.

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Section: Discussioncontrasting

confidence: 97%

Section: Discussioncontrasting

confidence: 97%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

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Host‐microbiota interactions shed light on mortality events in the striped venus clam Chamelea gallina

et al. 2019

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Transcriptome profiling analysis of Mactra veneriformis by deep sequencing after exposure to 2,2′,4,4′-tetrabromodiphenyl ether

et al. 2017

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Molecular biomarker responses in the freshwater mussel Anodonta anatina exposed to an industrial wastewater effluent

Ugge

Jonsson

Berglund

2021

Environ Sci Pollut Res

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Using a selection of molecular biomarkers, we evaluated responses in freshwater mussels (Anodonta anatina) exposed to effluent from an industrial wastewater treatment facility. The aims of this work were to (1) assess biomarkers of general toxicity under sublethal exposure to an anthropogenic mixture of chemicals, represented by an arbitrary effluent, and (2) evaluate the potential of A. anatina as a bioindicator of pollution. Adult mussels (n = in total 32; 24 males and 8 females) were exposed (96 h) in the laboratory to a fixed dilution of effluent or to a control treatment of standardized freshwater. Metal concentrations were in general higher in the effluent, by an order of magnitude or more, compared to the control. Toxic unit estimates were used as proxies of chemical stress, and Cu, Ni, and Zn were identified as potential major contributors (Cu> Ni > Zn). Six transcriptional (cat, gst, hsp70, hsp90, mt, sod) and two biochemical (AChE, GST) biomarkers were analyzed in two tissues, gills, and digestive glands. Out of the 16 responses (eight biomarkers × two tissues), 14 effect sizes were small (within ± 28 % of control) and differences non-significant (p > 0.05). Results did however show that (1) AChE activity increased by 40% in gills of exposed mussels compared to control, (2) hsp90 expression was 100% higher in exposed female gills compared to control, and (3) three marker signals (AChE in both tissues, and hsp70 in gills) differed between sexes, independent of treatment. Results highlight a need for further investigation of molecular biomarker variability and robustness in A. anatina.

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Can ecological history influence response to pollutants? Transcriptomic analysis of Manila clam collected in different Venice lagoon areas and exposed to heavy metal

Cited by 28 publications

References 69 publications

Host‐microbiota interactions shed light on mortality events in the striped venus clam Chamelea gallina

Host‐microbiota interactions shed light on mortality events in the striped venus clam Chamelea gallina

Transcriptome profiling analysis of Mactra veneriformis by deep sequencing after exposure to 2,2′,4,4′-tetrabromodiphenyl ether

Molecular biomarker responses in the freshwater mussel Anodonta anatina exposed to an industrial wastewater effluent

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