Can Insect-Based Diets Affect Zebrafish (<i>Danio rerio</i>) Reproduction? A Multidisciplinary Study

Randazzo, Basilio; Zarantoniello, Matteo; Gioacchini, Giorgia; Giorgini, Elisabetta; Truzzi, Cristina; Notarstefano, Valentina; Cardinaletti, Gloriana; Huyen, Kieu Thi; Carnevali, Oliana; Olivotto, Iacopo

doi:10.1089/zeb.2020.1891

Cited by 18 publications

(15 citation statements)

References 83 publications

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“…Nine samples of liver and medium and distal intestine were collected for each dietary group and immediately stored at −80 • C. To perform Fourier Transform Infrared Imaging (FTIRI) measurements, for each sample three sections (10 µm thick) were cut using a cryotome 200 µm apart from each other; the sections were deposited without any fixation process onto CaF2 optical windows (1 mm thick, 13 mm in diameter) and then left to air-dry for 30 min [59][60][61].…”

Section: Fourier Transform Infrared Imaging (Ftiri) Spectroscopy Measurements and Data Analysismentioning

confidence: 99%

Hermetia illucens and Poultry by-Product Meals as Alternatives to Plant Protein Sources in Gilthead Seabream (Sparus aurata) Diet: A Multidisciplinary Study on Fish Gut Status

Randazzo

Zarantoniello

Cardinaletti

et al. 2021

Animals

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The attempt to replace marine-derived ingredients for aquafeed formulation with plant-derived ones has met some limitations due to their negative side effects on many fish species. In this context, finding new, sustainable ingredients able to promote fish welfare is currently under exploration. In the present study, poultry by-products and Hermetia illucens meal were used to replace the vegetable protein fraction in diets totally deprived of fish meal intended for gilthead seabream (Sparus aurata). After a 12-week feeding trial, a multidisciplinary approach including histological, molecular, and spectroscopic techniques was adopted to investigate intestine and liver responses to the different dietary formulations. Regardless of the alternative ingredient used, the reduction in dietary vegetable proteins resulted in a lower incidence of intestine histological alterations and inflammatory responses. In addition, the dietary inclusion of insect meal positively affected the reduction in the molecular inflammatory markers analyzed. Spectroscopic analyses showed that poultry by-product meal improved lipid absorption in the intestine, while insect meal induced increased liver lipid deposition in fish. The results obtained demonstrated that both poultry by-products and H. illucens meal can successfully be used to replace plant-derived ingredients in diets for gilthead seabream, promoting healthy aquaculture.

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Section: Fourier Transform Infrared Imaging (Ftiri) Spectroscopy Measurements and Data Analysismentioning

confidence: 99%

Hermetia illucens and Poultry by-Product Meals as Alternatives to Plant Protein Sources in Gilthead Seabream (Sparus aurata) Diet: A Multidisciplinary Study on Fish Gut Status

Randazzo

Zarantoniello

Cardinaletti

et al. 2021

Animals

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“…Ovaries were processed according to Randazzo et al ( 34 ). Briefly, after dehydration in graded EtOH solutions, samples were clarified in xylene and embedded in paraffin.…”

Section: Methodsmentioning

confidence: 99%

Effects of Di-Isononyl Phthalate (DiNP) on Follicular Atresia in Zebrafish Ovary

et al. 2021

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Di-isononyl phthalate (DiNP) is a plasticizer reported to elicit hormone-like activity and disrupt metabolism and reproduction in fish and other vertebrates. In general, phthalates have been used at high concentrations beyond reported environmental levels to assess their adverse effects on fish gonadal physiology. The present study exposed adult female zebrafish to a wide range of DiNP concentrations [0.42 µg L−1 (10−9 M), 4.2 µg L−1 (10−8 M), and 42 µg L−1 (10−7 M)] for 21 days. We evaluated gene expression profiles related to apoptosis, autophagy, and oxidative stress; DNA fragmentation (TUNEL assay: terminal deoxynucleotidyl transferase dUTP nick end labeling) and caspase activity (CAS3) were also examined. Exposure to 0.42 and 4.2 µg L−1 upregulated the genes coding for tnfa and baxa, sod1, prkaa1, respectively. CAS3 immunohistochemistry revealed a higher number of positive vitellogenic oocytes in ovaries exposed to 0.42 µg L−1. Subsequently, we examined the relationship between CAS3 signaling and DNA fragmentation. Accordingly, DNA fragmentation was observed in vitellogenic follicles of fish exposed to 0.42 and 4.2 μg L−1. Our results demonstrate that follicular atresia can occur after exposure to environmental levels of DiNP for 21 days, which may adversely affect the reproductive performance of female zebrafish in a non-monotonic manner.

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“…This potentiality coupled with the optical microscopy allows to perform the hyperspectral imaging analysis of selected areas inside the analysed samples. In addition, this tool lets combine the topographical distribution of the most relevant biomolecules (in terms of lipids, proteins, carbohydrates and nucleic acids) with meaningful information on the biochemical composition and the occurrence of specific biological mechanisms and pathways (Giorgini et al, 2018a;Randazzo et al, 2020a;Zarantoniello et al, 2020a).…”

Section: Fish Gut and Liver Histologymentioning

confidence: 99%

“…Recently FTIR was used to characterise the macromolecular composition of class IV oocytes of zebrafish adult females fed over a 12 months period on BSF-based diets (Randazzo et al, 2020a). Specifically, while similar amounts of SFA, MUFA and PUFA were found in control and Hi25 groups, Hi50 showed higher SFA and lower PUFA contents.…”

Section: Fish Gut and Liver Histologymentioning

confidence: 99%

Application of laboratory methods for understanding fish responses to black soldier fly (Hermetia illucens) based diets

Zarantoniello

Randazzo

Secci

et al. 2022

JIFF

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A major challenge for development of sustainable aquafeeds is its dependence on fish meal and fish oil. Replacement with more sustainable, nutritious and safe ingredients is now a priority. Over the last years, among several alternatives proposed, insects have received great attention as possible candidates. In particular, the black soldier fly (Hermetia illucens; BSF) represents a concrete example of how the circular economy concept can be applied to fish culture, providing a valuable biomass rich in fat and protein valorising organic by-products. In the last decade, several studies have been published about the use of different BSF dietary inclusion levels for various fish species including experimental models. Varying and encouraging results have been obtained in this research field using a plethora of laboratory methodological approaches that can be applied and coupled to obtain a comprehensive view of the BSF-based diets effects on fish physiology, health, and quality. The present review aims to explore some of the most promising laboratory approaches like histology, infrared spectroscopy, gut microbiome sequencing, molecular biology, fish fillets’ physico-chemical and sensory properties, essential for a better understanding of fish welfare and fillet quality, when BSF is used as aquafeed ingredient. In particular, great importance has been given to European finfish species and experimental models.

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Can Insect-Based Diets Affect Zebrafish (Danio rerio) Reproduction? A Multidisciplinary Study

Cited by 18 publications

References 83 publications

Hermetia illucens and Poultry by-Product Meals as Alternatives to Plant Protein Sources in Gilthead Seabream (Sparus aurata) Diet: A Multidisciplinary Study on Fish Gut Status

Hermetia illucens and Poultry by-Product Meals as Alternatives to Plant Protein Sources in Gilthead Seabream (Sparus aurata) Diet: A Multidisciplinary Study on Fish Gut Status

Effects of Di-Isononyl Phthalate (DiNP) on Follicular Atresia in Zebrafish Ovary

Application of laboratory methods for understanding fish responses to black soldier fly (Hermetia illucens) based diets

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