Can Interferon-γ Release Assays Be Useful for Monitoring the Response to Anti-tuberculosis Treatment?: A Systematic Review and Meta-analysis

Pourakbari, Babak; Mamishi, Setareh; Benvari, Sepideh; Sauzullo, Ilaria; Bedini, Andrea; Valentini, Piero; Keicho, Naoto; Mahmoudi, Shima

doi:10.1007/s00005-020-00568-4

Cited by 10 publications

(4 citation statements)

References 37 publications

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“…The following studies indicated that “CD27 − IFN-γ + CD4 + ” correlated with the treatment of TB. Examinations based on T cell responses (TST, T-SPOT, IGRAs) have been widely researched and used in the auxiliary diagnosis of TB [ 25 – 27 ]. Recently, studies have indicated that they have no roles in the discrimination between latent infection and active TB [ 28 ].…”

Section: Discussionmentioning

confidence: 99%

Assessment of CD27 expression on T-cells as a diagnostic and therapeutic tool for patients with smear-negative pulmonary tuberculosis

Zhang

et al. 2021

BMC Immunol

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Background There is a global focus on illness diagnosis in smear-negative and latent tuberculosis infectious populations (SN-TB and LTBI). CD27 has been suggested to play a direct role in active TB. Little is known about smear-negative individuals. Here, we tried to investigate whether it has a role in smear-negative populations. The expression of CD27 and MTB-specific CD27 in CD4+ T cells (“CD27−CD4+” and “CD27−IFN-γ+CD4+”) was evaluated in MTB-unexposed controls (HC), TB contacts (TB-C) and SN-TB individuals by flow cytometry. The sensitivity, specificity and AUC (area under curve) of “CD27−IFN-γ+CD4+” cells to distinguish SN-TBs from HCs and TB-Cs were determined by receiver operating characteristic (ROC) curve analysis. The clinical index was selected from the clinical laboratory and evaluated for correlation with “CD27−IFN-γ+CD4+” cells by Spearman statistical analysis. Results We observed that the percentages of “CD27−IFN-γ+CD4+” cells were significantly increased in the SN-TB group compared with the HC and TB-C groups (AUC was 0.88, sensitivity was 82.14%, specificity was 80.00%, and P < 0.0001). The percentage of “CD27−IFN-γ+CD4+” cells was negatively correlated with WBC (white blood cell count) (r = − 0.3019, P = 0.0182) and positively correlated with IgE (immunoglobulin E) (r = 0.2805, P = 0.0362). Furthermore, “CD27−IFN-γ+CD4+” cells were significantly decreased, especially in the > 50 years group, after clinical treatment. Conclusion The present results demonstrated that the percentage of “CD27−IFN-γ+CD4+” cells might be a conceivable molecular indicator in the diagnosis of SN-TB and was influenced by its outcome of therapy.

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Section: Discussionmentioning

confidence: 99%

Assessment of CD27 expression on T-cells as a diagnostic and therapeutic tool for patients with smear-negative pulmonary tuberculosis

Zhang

et al. 2021

BMC Immunol

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“…The kit is based on the ELISA method and includes a negative control tube (Nil), a positive control tube (Mitogen), and a TB antigen tube with ESAT-6 and CFP-10 antigens. In previous studies, Wantai TB-IGRA showed no significant difference in sensitivity compared to QFT-GIT and T-SPOT [ 210 , 211 ]. In addition, Wantai TB-IGRA is relatively low-cost.…”

Section: Status and Challenges Of Discriminating Diagnosis Of Ltbimentioning

confidence: 97%

From immunology to artificial intelligence: revolutionizing latent tuberculosis infection diagnosis with machine learning

Li,

Yang,

Zhuang

et al. 2023

Military Med Res

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Latent tuberculosis infection (LTBI) has become a major source of active tuberculosis (ATB). Although the tuberculin skin test and interferon-gamma release assay can be used to diagnose LTBI, these methods can only differentiate infected individuals from healthy ones but cannot discriminate between LTBI and ATB. Thus, the diagnosis of LTBI faces many challenges, such as the lack of effective biomarkers from Mycobacterium tuberculosis (MTB) for distinguishing LTBI, the low diagnostic efficacy of biomarkers derived from the human host, and the absence of a gold standard to differentiate between LTBI and ATB. Sputum culture, as the gold standard for diagnosing tuberculosis, is time-consuming and cannot distinguish between ATB and LTBI. In this article, we review the pathogenesis of MTB and the immune mechanisms of the host in LTBI, including the innate and adaptive immune responses, multiple immune evasion mechanisms of MTB, and epigenetic regulation. Based on this knowledge, we summarize the current status and challenges in diagnosing LTBI and present the application of machine learning (ML) in LTBI diagnosis, as well as the advantages and limitations of ML in this context. Finally, we discuss the future development directions of ML applied to LTBI diagnosis.

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“…A systematic review reported high variation in reversion rates of IGRAs in either active or latent TB among studies, which makes these tests unreliable for monitoring response to antituberculous therapy (ATT) [32] . However, an advantage of QFT-Plus in monitoring the response to ATT was recently reported [33][34] . A decrease in IFN-γ response following QFT-Plus-TB2 stimulation was observed after subjects with active TB had received ATT.…”

Section: Advantages and Disadvantages Of Interferon-gammamentioning

confidence: 99%

Interferon-gamma release assays in tuberculous uveitis: a comprehensive review

Tungsattayathitthan¹,

Tesavibul²,

Dharakul³

et al. 2022

Int J Ophthalmol

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Tuberculous uveitis (TBU) comprises a broad clinical spectrum of ocular manifestations, making its diagnosis challenging. Ophthalmologists usually require evidence from investigations to confirm or support a clinical diagnosis of TBU. Since direct isolation of the causative organism from ocular specimens has limitations owing to the small volume of the ocular specimens, resultant test positivities are low in yield. Immunodiagnostic tests, including the tuberculin skin test and interferon-gamma release assays (IGRAs), can help support a clinical diagnosis of TBU. Unlike the tuberculin skin test, IGRAs are in vitro tests that require a single visit and are not affected by prior Bacillus Calmette-Guerin vaccination. Currently, available IGRAs consist of different techniques and interpretation methods. Moreover, newer generations have been developed to improve the sensitivity and ability to detect active tuberculosis. This narrative review collates salient practice points as a reference for general ophthalmologists, such as evidence for the utilization of IGRAs in patients with suspected TBU, and summarizes basic knowledge and details of clinical applications of these tests in a clinical setting.

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Can Interferon-γ Release Assays Be Useful for Monitoring the Response to Anti-tuberculosis Treatment?: A Systematic Review and Meta-analysis

Cited by 10 publications

References 37 publications

Assessment of CD27 expression on T-cells as a diagnostic and therapeutic tool for patients with smear-negative pulmonary tuberculosis

Assessment of CD27 expression on T-cells as a diagnostic and therapeutic tool for patients with smear-negative pulmonary tuberculosis

From immunology to artificial intelligence: revolutionizing latent tuberculosis infection diagnosis with machine learning

Interferon-gamma release assays in tuberculous uveitis: a comprehensive review

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