Can intrauterine contraceptive devices be a Candida albicans reservoir?

Chassot, Francieli; Negri, Melyssa; Svidzinski, Arthur Estivalet; Donatti, Lucélia; Peralta, Rosane Marina; Svidzinski, Terezinha Inez Estivalet; Consolaro, Márcia Edilaine Lopes

doi:10.1016/j.contraception.2008.01.007

Cited by 68 publications

(58 citation statements)

References 16 publications

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“…Concerning VVC infection, the clinical C. albicans isolates from VVC patients have been shown in vitro the ability to adhere to the surfaces of both microtiter plates and intrauterine contraceptive devices [40,41]. Herein, the combination of QCT and FCZ could significantly inhibit the adherence of VVC isolate (Fig.…”

Section: Discussionmentioning

confidence: 99%

Quercetin Assists Fluconazole to Inhibit Biofilm Formations of Fluconazole-Resistant Candida Albicans in In Vitro and In Vivo Antifungal Managements of Vulvovaginal Candidiasis

Gao

Wang

Zhu

2016

Cell Physiol Biochem

104

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Background: Vulvovaginal candidiasis (VVC) is a common gynecological disease. Candida albicans is believed to be mainly implicated in VVC occurrence, the biofilm of which is one of the virulence factors responsible for resistance to traditional antifungal agents especially to fluconazole (FCZ). Quercetin (QCT) is a dietary flavonoid and has been demonstrated to be antifungal against C. albicans biofilm. Methods: 17 C. albicans isolates including 15 clinical ones isolated from VVC patients were employed to investigate the effects of QCT and/or FCZ on the inhibition of C. albicans biofilm. Results: We observed that 64 µg/mL QCT and/or 128 µg/mL FCZ could (i) be synergistic against 10 FCZ-resistant planktonic and 17 biofilm cells of C. albicans, (ii) inhibit fungal adherence, cell surface hydrophobicity (CSH), flocculation, yeast-to-hypha transition, metabolism, thickness and dispersion of biofilms; (iii) down-regulate the expressions of ALS1, ALS3, HWP1, SUN41, UME6 and ECE1 and up-regulate the expressions of PDE2, NRG1 and HSP90, and we also found that (iv) the fungal burden was reduced in vaginal mucosa and the symptoms were alleviated in a murine VVC model after the treatments of 5 mg/kg QCT and/or 20 mg/kg FCZ. Conclusion: Together with these results, it could be demonstrated that QCT could be a favorable antifungal agent and a promising synergist with FCZ in the clinical management of VVC caused by C. albicans biofilm.

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Section: Discussionmentioning

confidence: 99%

Quercetin Assists Fluconazole to Inhibit Biofilm Formations of Fluconazole-Resistant Candida Albicans in In Vitro and In Vivo Antifungal Managements of Vulvovaginal Candidiasis

Gao

Wang

Zhu

2016

Cell Physiol Biochem

104

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“…Almost all kind of surfaces are suitable to be colonized by biofilms (Donlan & Costerton, 2002). Biomedical devices are not the exception, biofilms are often found on the surface of urinary catheters (Stickler, 2008), central venous catheters (Petrelli et al, 2006), heart valves (Litzler et al, 2007), voice prostheses (Buijssen et al, 2007), contact lenses (Imamura et al, 2008), hip prostheses (Dempsey et al, 2007) and intrauterine devices (Chassot et al, 2008). Current biofilm preventive strategies are essentially aimed at coating medical surfaces with antimicrobial agents, a process not always successful (Basak et al, 2009;von Eiff et al, 2005).…”

Section: Biofilms On Medical Devicesmentioning

confidence: 99%

Biosurfactants and Bioemulsifiers Biomedical and Related Applications – Present Status and Future Potentials

Fracchia¹,

Cavallo²,

Martinotti³

et al. 2012

Biomedical Science, Engineering and Technology

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“…Scanning electron microscopy (SEM) was used in ex vivo experiments to show that yeast isolates from vaginal exudates of patients with vulvovaginal candidiasis were able to adhere to a combined contraceptive vaginal ring (NuvaRing; Organon Pharmaceuticals) (Camacho et al, 2007) and to contraceptive intrauterine devices (Chassot et al, 2008), with concomitant biofilm formation. Infected intrauterine devices recovered from patients suffering from reproductive tract infections were found to be tainted with Candida biofilm (Lal et al, 2008).…”

Section: Introductionmentioning

confidence: 99%

Microbial biofilms on the surface of intravaginal rings worn in non-human primates

Gunawardana

Moss

Smith

et al. 2011

Journal of Medical Microbiology

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Millions of intravaginal rings (IVRs) are used by women worldwide for contraception and for the treatment of vaginal atrophy. These devices also are suitable for local and systemic sustained release drug delivery, notably for antiviral agents in human immunodeficiency virus pre-exposure prophylaxis. Despite the widespread use of IVRs, no studies have examined whether surfaceattached bacterial biofilms develop in vivo, an important consideration when determining the safety of these devices. The present study used scanning electron microscopy, fluorescence in situ hybridization and confocal laser scanning microscopy to study biofilms that formed on the surface of IVRs worn for 28 days by six female pig-tailed macaques, an excellent model organism for the human vaginal microbiome. Four of the IVRs released the nucleotide analogue reverse transcriptase inhibitor tenofovir at a controlled rate and the remaining two were unmedicated. Large areas of the ring surfaces were covered with monolayers of epithelial cells. Two bacterial biofilm phenotypes were found to develop on these monolayers and both had a broad diversity of bacterial cells closely associated with the extracellular material. Phenotype I, the more common of the two, consisted of tightly packed bacterial mats approximately 5 mm in thickness. Phenotype II was much thicker, typically 40 mm, and had an open architecture containing interwoven networks of uniform fibres. There was no significant difference in biofilm thickness and appearance between medicated and unmedicated IVRs. These preliminary results suggest that bacterial biofilms could be common on intravaginal devices worn for extended periods of time.

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Can intrauterine contraceptive devices be a Candida albicans reservoir?

Cited by 68 publications

References 16 publications

Quercetin Assists Fluconazole to Inhibit Biofilm Formations of Fluconazole-Resistant Candida Albicans in In Vitro and In Vivo Antifungal Managements of Vulvovaginal Candidiasis

Quercetin Assists Fluconazole to Inhibit Biofilm Formations of Fluconazole-Resistant Candida Albicans in In Vitro and In Vivo Antifungal Managements of Vulvovaginal Candidiasis

Biosurfactants and Bioemulsifiers Biomedical and Related Applications – Present Status and Future Potentials

Microbial biofilms on the surface of intravaginal rings worn in non-human primates

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