Can lactoferrin modulate the immunostimulant activity of levamisole in rats immunosuppressed by cyclophosphamide?

Abstract

ÖZETAmaç: Bu çalışmanın amacı immunsuprese rat modelinde laktoferrin kullanılarak levamizolün immün düzenleyici aktivitesini araştırmaktır.Yöntemler: Çalışma planı: Çalışmada 14 haftalık 140 erkek albino rat (250-280 g) kullanıldı. Ratlar herbirinde 20 rat bulunan 7 gruba ayrıldı. Birinci grup Kontrol grubu, Grup II'ye siklofosfamid tek intraperitoneal dozda (250 mg), Grup III siklofosfamid ve laktoferrin verilen grup, Grup IV oral içme suyu içinde sadece Laktoferrin (%0,5) verilen grup; Grup V siklofosfamid ve levamizol ile tedavi edildi, Grup VI'ya oral levamizol (2,5 mg/kg) verildi ve GrupVII'ye laktoferrin, siklofosfamid ve lavamizol verildi. Hayvanlar feda edildi ve çalışmanın başlamasını takib eden 21. Günde iki farklı kan örneği alındı ve total ve ayırıcı lökosit sayısı, serum total proteinleri, albümin, alfa globülin, beta globülin, gama globülin, nitrik oksit üretimi ve lizozim aktivitesi ölçüldü. Bulgular:Siklofosfamid grubunun yukarıda bahsedilen parametrelerinde anlamlı düşüş gözlendi ki bu durum hem laktoferrin hem de levamizol verilmesi ile düzeldi.Sonuç: Bu çalışmamızda siklofosfamid ile immunsuprese edilen ratlarda laktoferrinin levamizolün immunstimulan etkisini iyileştirdiği sonucuna varıldı.Anahtar kelimeler: Sığır laktoferrini, Levamizol, Siklofosfamid, Immunmodülasyon, Lizozim deneyi ABSTRACT Objective: The aim of this study was to study the immunomodulatory activity improvement of levamisole by using lactoferrin when applied to immunosuppressed rat model. Methods:The study was designed as follows, 140 male albino rats (250-280 g) 14 weeks old were used in our work. Rats were randomly divided into seven groups, 20 in each. The group I was kept as a control, group II was given cyclophosphamide (CYP) at a single intraperitoneal dose of (250 mg/kg body weight), group III CYP and lactoferrin (Lac) treated group, group IV orally administrated Lac only (0.5%) in drinking water, group V treated with CYP and levamisole, group VI administrated levamisole orally at a dose of (2.5 mg/kg body weight) and group VII was given CYP, Lac and levamisole. Animals were sacrificed and two separate blood samples were collected after 21 days from the beginning of the experiment for measuring the total and differential leukocyte count, serum total proteins, albumin, alpha globulin, beta globulin and gamma globulin, Nitric oxide (NO) production and lysozyme activity.Results: CYP group showed significant decrease in the above mentioned parameters, which were improved after administration of both lactoferrin and levamisole. Conclusion:Our study concluded that lactoferrin improve the immunostimulant effect of levamisole in CYPimmunosuppressed rats. J Clin Exp Invest 2014; 5 (1): 48-53

Effect of Lactoferrin on Paraoxonase Activity, Some Acute Phase Proteins and Oxidant/Antioxidant System

“…This study shows similar results compared to the data we have obtained in our study. In another study, it was observed that there was no significant difference in total protein, α-and β-globulin levels in oral drinking water administration of lactoferrin (0.5%), but a significant increase (P<0.001) in albumin levels in rats (Mohamed, 2014). Similarly, in this study, it was found that serum albumin, total protein and globulin levels in rats did not change through lactoferrin administration compared to control.…”

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“…Therefore, it can be used as a drug that can cause a measurable immunеdeficiency state (Akhter et. al., 2008;Ukpo, 2013;Mohamed, 2014;Lee et. al., 2019).…”