Can permeable super oxide dismutase mimetic agents improve the quality of frozen–thawed ram semen?

Forouzanfar, Mohsen; Ershad, Saman Fekri; Hosseini, Sayyed Morteza; Hajian, Mehdi; Ostadhosseini, S.; Abid, Abdolah; Tavalaee, Marziyeh; Shahverdi, Abdolhossein; Dizaji, Ahmad Vosough; Nasr-Esfahani, Mohammad Hossein

doi:10.1016/j.cryobiol.2012.12.006

Cited by 38 publications

(25 citation statements)

References 29 publications

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“…Exactly the same has been found for porcine and rooster spermatozoa cryopreserved in the presence of supplementary SOD and catalase [78,79]. SOD mimetics have also been shown to improve the cryopreservation of alpaca and ram spermatozoa and to improve subsequent blastocyst formation rates in the goat [80][81][82]. The combination of SOD mimetic and catalase was, predictably, more effective than mimetic alone [83].…”

Section: Cryostoragementioning

confidence: 60%

The Importance of Oxidative Stress in Determining the Functionality of Mammalian Spermatozoa: A Two-Edged Sword

2020

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This article addresses the importance of oxidative processes in both the generation of functional gametes and the aetiology of defective sperm function. Functionally, sperm capacitation is recognized as a redox-regulated process, wherein a low level of reactive oxygen species (ROS) generation is intimately involved in driving such events as the stimulation of tyrosine phosphorylation, the facilitation of cholesterol efflux and the promotion of cAMP generation. However, the continuous generation of ROS ultimately creates problems for spermatozoa because their unique physical architecture and unusual biochemical composition means that they are vulnerable to oxidative stress. As a consequence, they are heavily dependent on the antioxidant protection afforded by the fluids in the male and female reproductive tracts and, during the precarious process of insemination, seminal plasma. If this antioxidant protection should be compromised for any reason, then the spermatozoa experience pathological oxidative damage. In addition, situations may prevail that cause the spermatozoa to become exposed to high levels of ROS emanating either from other cells in the immediate vicinity (particularly neutrophils) or from the spermatozoa themselves. The environmental and lifestyle factors that promote ROS generation by the spermatozoa are reviewed in this article, as are the techniques that might be used in a diagnostic context to identify patients whose reproductive capacity is under oxidative threat. Understanding the strengths and weaknesses of ROS-monitoring methodologies is critical if we are to effectively identify those patients for whom treatment with antioxidants might be considered a rational management strategy.

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Section: Cryostoragementioning

confidence: 60%

The Importance of Oxidative Stress in Determining the Functionality of Mammalian Spermatozoa: A Two-Edged Sword

2020

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“…This includes, antioxidant enzymes acting at the extracellular level, such as catalase [Li et al 2010] and SOD [Forouzanfar et al 2013;Roca et al 2005]; non-enzymatic antioxidants counteracting ROS generation at cell membrane, such as vitamin E [Kalthur et al 2011]; and non-enzymatic antioxidants increasing intracellular antioxidant capacity, such as glutathione (GSH) [Gadea et al 2011] or genistein [Martinez-Soto et al 2010].…”

Section: Discussionmentioning

confidence: 99%

Addition of Tempol in semen cryopreservation medium improves the post-thaw sperm function

Bateni¹,

Azadi²,

Tavalaee³

et al. 2014

Systems Biology in Reproductive Medicine

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Despite extensive research carried out for optimization and commercialization of sperm cryopreservation media, percentage of motility and viability remain low following cryopreservation. These adverse effects have been partially ascribed to reactive oxygen species (ROS) production during cryopreservation. Therefore, we proposed that addition of a cell permeable antioxidant like Tempol, with superoxide dismutase (SOD) mimetic action, may overcome these effects in an optimized commercially available cryo-protective medium. Therefore, semen samples were cryopreserved in the presence or absence of Tempol. A concentration of 5 μM Tempol was defined as optimal since it significantly improved motility and viability post thawing and reduced DNA fragmented sperm. In addition, percentage of ROS positive sperm was reduced. These effects of Tempol can be attributed to cell permeability characteristic and ability to reduce superoxide production both at intra- and extra-cellular levels. Tempol may hold the potential for clinical applications.

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“…Two antioxidant agents that mimic superoxide dismutase (Tempo and Tempol) displayed protective effect on sperm DNA fragmentation, after their addition on ram semen extender during the cooling process (Mata-Campuzano et al, 2012;Santiani et al, 2014). Additionally, it has been shown that 150 μM of a cell-permeable superoxide dismutase mimetic and peroxynitrite scavenger (MnTBAP), reduced the oxidative stress provoked by cryopreservation, leading to higher sperm motility and improved acrosomal membrane integrity (Forouzanfar et al, 2013). Moreover, Maia et al (2006; reported a valuable impact of catalase on frozen-thawed ram spermatozoa's motility and stability of both acrosome and plasma membranes.…”

Section: I) Enzymatic Antioxidantsmentioning

confidence: 99%

“…Spermatozoa regulate and resist to this phenomenon by an antioxidant protective system with the cooperation of seminal plasma, membranes, and cytoplasm, including superoxide dismutase, glutathione peroxidase and catalase. However, this system is partly removed and severely altered during cryopreservation procedures (Marti et al, 2008;Forouzanfar et al, 2013). A variety of biological and chemical antioxidants are presently under investigation, aiming to avoid the detrimental effects of oxidative stress on semen quality.…”

Section: ) Research Concerning "Antioxidants" Additionmentioning

confidence: 99%

Current status and advances in ram semen cryopreservation

Ntemka

Tsakmakidis

Kiossis

et al. 2018

J Hellenic Vet Med Soc

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Ram semen cryopreservation contributes to genetic improvement through artificial insemination, eliminates geographical barriers in artificial insemination application and supports the preservation of endangered breeds thus the conservation of biodiversity. Sperm freezing process induces ultrastructural, biochemical and functional changes of spermatozoa. Especially, spermatozoa’s membranes and chromatin can be damaged, sperm membranes’ permeability is increased, hyper oxidation and formation of reactive oxygen species takes place, affecting fertilizing ability and subsequent early embryonic development. Aiming to improve ram frozen-thawed semen’s fertilizing capacity, many scientific investigations took place. Among them the composition of semen extenders, was a main point of interest. Semen preservation extenders regulate and support an environment of adequate pH and buffering capacity to protect spermatozoa from osmotic and cryogenic stress. Therefore, permeating (glycerol, dimethyl sulfoxide) and non-permeat ing (egg yolk, skimmed milk) cryoprotectants, sugars (glucose, lactose, trehalose, raffinose), salts (sodium citrate, citric acid) and antioxidants (amino acids, vitamins, enzymes) have been added and tested. Moreover, semen dilution rate, storage temperature, cooling rate and thawing protocol, are also some key factors that have been studied. The research results of this scientific topic are encouraging, not only about the freezing and thawing procedures, but also about the improvement of the additives’ properties. However, further research is needed to enhance the fertilizing ability of ram frozen-thawed semen, making its use practical in sheep reproductive management by the application of cervical artificial insemination.

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Can permeable super oxide dismutase mimetic agents improve the quality of frozen–thawed ram semen?

Cited by 38 publications

References 29 publications

The Importance of Oxidative Stress in Determining the Functionality of Mammalian Spermatozoa: A Two-Edged Sword

The Importance of Oxidative Stress in Determining the Functionality of Mammalian Spermatozoa: A Two-Edged Sword

Addition of Tempol in semen cryopreservation medium improves the post-thaw sperm function

Current status and advances in ram semen cryopreservation

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