Can regular moderate exercise lead to changes in miRNA-146a and its adapter proteins in the kidney of streptozotocin-induced diabetic male rats?

Abstract

Objective. Th e aim of this study was to assess whether microRNA-146a and its adapter proteins TNF receptor associated factor6 (TRAF6) and interleukin-1 receptor-associated kinase-1 (IRAK1) may be changed in the kidney of streptozotocin-induced diabetic rats, following regular moderate exercise.Methods. Forty adult male Wistar rats were allocated randomly into four groups (n=10), including sedentary control (SC), sedentary diabetic (SD), healthy sixty-day exercise (H60E), and diabetic sixty-day exercise (D60E) groups. Diabetes was induced by an intraperitoneal injection of 60 mg/kg streptozotocin. Aft er 48 h, blood glucose levels >250 mg/dl was included to diabetic rats. Aft er 2 days of diabetes induction, the exercise protocol began. Animals were exposed to 5 days of consecutive treadmill exercise for 60 min/day with the 22 m/min speed for 60 days. Th e kidneys of the rats were removed and microRNA was extracted from them using the miRCURYTM RNA isolation kit.Results. In diabetic rats, statistical analysis revealed a signifi cant decrease in miR-146a expression, non-signifi cant decrease in IRAK1 mRNA expression, and non-signifi cant increase in TRAF6 and NF-kB mRNA expression compared to the SC group. Exercise led to a non-signifi cant increase in the expression of miR-146a and NF-kB mRNA in the kidneys of the diabetic group as compared to the SD group, signifi cant increase in TRAF6 and IRAK1 mRNA expression compared to the H60E group, and signifi cant increase in TRAF6 mRNA expression compared to the SD group.Conclusion. Th e present data indicate that exercise might be able to help in the prevention in the diabetic nephropathy development.

Evaluation of ameliorative effect of sodium nitrate in experimental model of streptozotocin-induced diabetic neuropathy in male rats

“…Three days later, blood (obtained from a tail vein) glucose levels were measured using a glucometer (Norditalia Elettromedicali S.r.I., Italy). Animals with glucose levels more than 250 mg/dl were considered diabetic and those with glucose levels less than 250 mg/dl were excluded from the study (Oghbaei et al 2017).…”

Sodium nitrate preconditioning prevents progression of the neuropathic pain in streptozotocin-induced diabetes Wistar rats

“…Blood samples for glucose measurements were taken from the tail vein 72 h following the STZ injection. The rats with blood glucose more than 250 mg/dl were considered diabetic [27]. All treatments were started immediately after confirmation of diabetes for 60 days.…”

Exercise training ameliorates early diabetic kidney injury by regulating the H2S/SIRT1/p53 pathway

“…It has been reported that exercise is always found as an effective intervention to prevent diabetic complications, that is, depression, cardiomyopathy, and neuropathy 33,34 . Recent studies about diabetic patients and animal models suggest that exercise has a protective effect against the diabetic nephropathy, dependently or independently of glycemic control 35,36 . Clinical studies have demonstrated that aerobic exercise is beneficial to decrease urea, creatinine, blood pressure and improve the physical performance of diabetic patients 36,37 .…”