Can serums be replaced by Mueller-Hinton agar in germ tube test?

Atalay, Mustafa; Koç, Ayşe; Parkan, Ömür Mustafa; Aydemir, Gonca; Elmalı, Ferhan; Sav, Hafıze

doi:10.4103/1119-3077.180046

Cited by 6 publications

(6 citation statements)

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“…The possible cause of variability in germ tube positivity rate may be attributed to the incubation temperature andtime. 4,8,13,14 Trypticase soy broth had a sensitivity rate of 72.65% in our study; which is similar to the findings of Arora et al and Makwana et al 6,10 In contrast, Kim et al and Deorukhar et al had reported a higher sensitivity rates of 100% and 94%respectively in trypticase soya broth. 7 In this study, 61.71% of C. albicans isolates showed germ tube test positive in peptone water.…”

Section: Discussionsupporting

confidence: 90%

A comparative study for production of germ tube in Candida albicans of various pulmonary samples, by different methods in a tertiary care hospital of south west Rajasthan

Bhumbla

Gupta

2021

Int J Community Med Public Health

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Background: Systemic candidiasis is associated with a high crude mortality rate, even with first line antifungal therapy. C. albicans is the predominant cause of invasive fungal diseases which is a serious public health issue. The main objective was to assess the reliability of different media for germ tube production in Candida albicans isolated from various clinically diagnosed pulmonary samples.Methods: All Candida isolates were identified and speciated by conventional methods such as Gram’s staining, germ tube test, chlamydospore formation on corn meal agar, sugar fermentation test, sugar assimilation test, and growth on Hi-chrome candida agar.Results: Out of 108 clinical isolates of Candida albicans, 5 different methods were used for germ tube production. Pooled human sera showed 93/108 (86.1%) was the most sensitive method wherein YEPD (yeast extract peptone dextrose) broth 91/108 (84.7%) was the reliable and easy method for detection of germ tube, followed by trypticase soy broth 81/108 (81.4%); peptone water 80/108 (74.7%) and 2% sucrose 71/108 (65.7%).Conclusions: YPED broth is found to be a better serum free substrate and subsequently for the presumptive differentiation of C. albicans from non-albicans candida (NAC), without the extensive time required for the preparation and testing of pooled human serum. Furthermore, this medium is commercially available, more stable, effective, and is not bio hazardous.

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Section: Discussionsupporting

confidence: 90%

A comparative study for production of germ tube in Candida albicans of various pulmonary samples, by different methods in a tertiary care hospital of south west Rajasthan

Bhumbla

Gupta

2021

Int J Community Med Public Health

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“…Further, YEPD medium had sensitivity (81.25%) and also Kim et al, (2002) reported 100% positivity at 39º, comparing germ tube induction in rabbit serum at 37ºC.The possible cause of variability in germ tube positivity rate may be attributed to the incubation temperature and time. (Sachin C. Deorukhkar et al, 2012;Kim et al, 2002;Atalay et al, 2017) Trypticase soya broth had a sensitivity rate of 72.65% in our study; which is similar to the findings of (Arora et al, 2003) and (Gopee E. Makwana et al, 2012). In contrast, Joshi KR et al, and (Sachin C. Deorukhkar et al, 2012) had reported a higher sensitivity rates of 100% and 94%respectively in Trypticase soya broth.…”

Section: Resultssupporting

confidence: 82%

Comparison of Different Media for Germ Tube Production by Candida albicans: A Retrospective Study

Mehta¹,

Kumar²,

Bhumbla³

et al. 2018

Int.J.Curr.Microbiol.App.Sci

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“…Germ tube induction in agar media was conducted by dripping 10 μL of 0.5 McFarland fungi suspension into 1 x 1 cm 2 agar. 18 Cover slip was placed to facilitate easier examination. The agar was then incubated for three hours at 37°C.…”

Section: Gem Tube Inductionmentioning

confidence: 99%

“…Interestingly, Atalay et al (2017) found that MHA was the best media for germ tube induction compared to human serum. 18 Distinct factor affects the yeast-to-hyphal transition in agar media. Villa et al (2020) stated that C. albicans hyphal growth in agar is influenced by the embedded surroundings or conditions.…”

Section: Germ Tube Induction In Agarmentioning

confidence: 99%

Germ Tube Induction Test Comparing Total of Six Liquid and Three Solid Media in Candida albicans

Ruby

Arifin

Kurniawan

et al. 2023

IJTID

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Invasive candidiasis (IC) has a high mortality rate of 70%, thus diagnosis should be established without delay. Given its fast result, serological test such as β-d-glucan (BDG) test is one alternative diagnosis modalities. However, it lacks specificity. Candida albicans germ tube antibody (CAGTA) test is an alternative serological test which has a high sensitivity of 76.2% and specificity of 80.3%. Manufacturing CAGTA serological test requires provision of specific germ tube antigen. In this study, various culture media were tested to find the best media for germ tube induction. This study was an experimental in vitro study. The number and length of the germ tube were recorded in two- and three-hour incubation periods. A total of six samples containing one C. albicans ATCC 90028, four C. albicans wild type strains, and one C. krusei wild type strain were used. Nine media were tested to induce germ tube formation: human and sheep serum, fetal bovine serum, mueller hinton agar and broth, tryptic soy agar and broth, brain heart infusion agar and broth. At both incubation periods, the medium with the highest number of germ tube was human serum (p=0.001 and p=0). The longest germ tube was found in sheep serum at two-hour incubation period (p=0.005). Mueller hinton broth (MHB) showed comparable results with human and sheep serum (p>0.05). Human serum is a superior inducer of morphogenesis. However, the use of MHB is recommended in this study, since provision of fresh human and sheep serum on a regular basis is impractical.

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Can serums be replaced by Mueller-Hinton agar in germ tube test?

Abstract: It is suggested that inexpensive MHA is a fast, appropriate, and reliable medium for the probable diagnosis of GTT and C. albicans; however, additional studies are still needed to define other Candida species.

Cited by 6 publications

References 0 publications

A comparative study for production of germ tube in Candida albicans of various pulmonary samples, by different methods in a tertiary care hospital of south west Rajasthan

A comparative study for production of germ tube in Candida albicans of various pulmonary samples, by different methods in a tertiary care hospital of south west Rajasthan

Comparison of Different Media for Germ Tube Production by Candida albicans: A Retrospective Study

Germ Tube Induction Test Comparing Total of Six Liquid and Three Solid Media in Candida albicans

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