Can subgenomic mRNA predict course of COVID-19? – An observational study from a tertiary care center

Anand, Kuldip Parkash; Karade, Santosh; Sen, Sourav; Ray, Subrata Basu; Patil, Prashant; Thosani, Pratik; Shergill, S.P.S.; Gupta, Rajeev

doi:10.4103/jmms.jmms_170_20

Cited by 3 publications

(4 citation statements)

References 3 publications

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“…Transmission experiments should be further performed to understand the significance of this finding. In human COVID-19 cases, where the presence of sgRNA is studied, it is found that it is rarely detectable post 8 days of illness [ 27 , 28 ]. A study from England has shown increased household transmission associated with the Delta variant compared with the Alpha variant, which was previously reported to be highly transmissible [ 29 ].…”

Section: Discussionmentioning

confidence: 99%

“…The minimal lesions observed with other variants could be also because of the same reason, as we have observed severe lesions and body weight loss in hamsters infected with a dose of 10 6.5 TCID50/mL of the B.1 variant in our earlier studies [ 24 ]. The Delta variant has shown increased replication and enhanced entry efficiency in in vitro experiments [ 28 ]. An increased fitness advantage of the variant has been observed in the respiratory organoid system compared with wild type SARS-CoV-2 [ 28 ].…”

Section: Discussionmentioning

confidence: 99%

“…The Delta variant has shown increased replication and enhanced entry efficiency in in vitro experiments [ 28 ]. An increased fitness advantage of the variant has been observed in the respiratory organoid system compared with wild type SARS-CoV-2 [ 28 ]. Reports from England and Scotland have shown an increased risk of hospitalization in the case of the B.1.617.2 variant cases [ 7 ].…”

Section: Discussionmentioning

confidence: 99%

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SARS-CoV-2 Delta Variant Pathogenesis and Host Response in Syrian Hamsters

Mohandas

Yadav

Shete

et al. 2021

Viruses

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B.1.617 is becoming a dominant Severe Acute Respiratory Syndrome-Coronavirus-2 (SARS-CoV-2) lineage worldwide with many sublineages, of which B.1.617.2 is designated as a variant of concern. The pathogenicity of B.1.617.2 (Delta) and B.1.617.3 lineage of SARS-CoV-2 was evaluated and compared with that of B.1, an early virus isolate with D614G mutation in a Syrian hamster model. Viral load, antibody response, and lung disease were studied. There was no significant difference in the virus shedding pattern among these variants. High levels of SARS-CoV-2 sub genomic RNA were detected in the respiratory tract of hamsters infected with the Delta variant for 14 days, which warrants further transmission studies. The Delta variant induced lung disease of moderate severity in about 40% of infected animals, which supports the attributed disease severity of the variant. Cross neutralizing antibodies were detected in animals infected with B.1, Delta, and B.1.617.3 variant, but neutralizing capacity was significantly lower with B.1.351 (Beta variant).

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

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SARS-CoV-2 Delta Variant Pathogenesis and Host Response in Syrian Hamsters

Mohandas

Yadav

Shete

et al. 2021

Viruses

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“…Viral sg mRNA is considered to be an indicator of active infection [17]. In human COVID-19 cases, where presence of sgRNA is studied, it is found that its rarely detectable post 8 days of illness [23,24]. A study from England has shown increased household transmission associated with the Delta compared to the Alpha, which was reported to be highly transmissible earlier [25].…”

Section: Discussionmentioning

confidence: 99%

SARS-CoV-2 Delta variant pathogenesis and host response in Syrian hamsters

Mohandas

Yadav

Shete

et al. 2021

Preprint

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B.1.617 lineage is becoming a dominant SARS-CoV-2 lineage worldwide and was the dominant lineage reported in second COVID-19 wave in India, which necessitated studying the properties of the variant. We evaluated the pathogenicity and virus shedding of B.1.617.2 (Delta) and B.1.617.3 lineage of SARS-CoV-2 and compared with that of B.1, an early virus isolate with D614G mutation in Syrian hamster model. Viral load, antibody response and lung disease were studied. No significant difference in the virus shedding pattern was observed among these variants studied. A significantly high SARS-CoV-2 sub genomic RNA could be detected in the respiratory tract of hamsters infected with Delta variant for 14 days. Delta variant induced lung disease of moderate severity in 40% of infected animals. The neutralizing capability of the B.1, Delta and B.1.617.3 variant infected animals were found significantly lower with the B.1.351 (Beta variant). The findings of the study support the attributed disease severity and the increased transmission potential of the Delta variant.

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Speed versus Sensitivity

Sharma

Karade

Anand

et al. 2021

Journal of Marine Medical Society

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Background: As the world battles the coronavirus disease 2019 (COVID-19) pandemic, rapid detection is the key to contain the spread of the disease. Although real-time polymerase chain reaction (RT-PCR) remains the gold standard for the diagnosis of severe acute respiratory syndrome corona virus-2 (SARS-CoV-2) infection, it demands specialized equipment and technical expertise. SARS-CoV-2 rapid antigen detection test (RAT) is the user-friendly alternative, which needs evaluation in the hospital setting. Materials and Methods: This cross-sectional study was carried out at the Indian Council of Medical Research approved COVID-19 diagnostic molecular laboratory between November and December 2020. Nasopharyngeal swabs in viral transport medium (VTM) were received from suspected SARS-CoV-2 infected individuals were processed for SARS-CoV-2 RT-PCR and a rapid antigen detection test, COVID-19 Ag Respi-Strip. Results: A total of 342 samples were assessed for this study by RT-PCR and Rapid Antigen Test. The sensitivity of RAT was found to be 41% at an RT-PCR cycle threshold of 35 or below. Sensitivity of RAT improved to 81.2% when a RT-PCR cycle threshold of 25 or below was considered as positive. Specificity of RAT was 99% to 100%. Conclusion: Rapid antigen test is a cost-effective alternative for screening of symptomatic individuals in the hospital setting. However, due to low sensitivity, the negative results need to be interpreted with caution.

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Can subgenomic mRNA predict course of COVID-19? – An observational study from a tertiary care center

Cited by 3 publications

References 3 publications

SARS-CoV-2 Delta Variant Pathogenesis and Host Response in Syrian Hamsters

SARS-CoV-2 Delta Variant Pathogenesis and Host Response in Syrian Hamsters

SARS-CoV-2 Delta variant pathogenesis and host response in Syrian hamsters

Speed versus Sensitivity

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