Cancer organoids: A platform in basic and translational research

Ma, Xin; Wang, Qin; Li, Guozheng; Li, Hui; Xu, Shanhui; Pang, Da

doi:10.1016/j.gendis.2023.02.052

Cited by 11 publications

(2 citation statements)

References 203 publications

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“…The name ‘organoid’ accurately describes a miniaturised model of an entire organ. Organoids are commonly used in cancer research, with patient-derived organoids (PDOs) proving to be a valuable tool in drug testing and the study of cancer biology [ 56 , 57 , 58 ]. Additionally, organoids are utilised as models for a range of other diseases, including lung diseases [ 59 ], liver cirrhosis [ 60 ], inflammatory bowel disease [ 61 ], and brain disorders [ 62 ].…”

Section: 3d Cell Culturesmentioning

confidence: 99%

Biomimetic Scaffolds—A Novel Approach to Three Dimensional Cell Culture Techniques for Potential Implementation in Tissue Engineering

Górnicki,

Lambrinow,

Golkar-Narenji

et al. 2024

Nanomaterials

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Biomimetic scaffolds imitate native tissue and can take a multidimensional form. They are biocompatible and can influence cellular metabolism, making them attractive bioengineering platforms. The use of biomimetic scaffolds adds complexity to traditional cell cultivation methods. The most commonly used technique involves cultivating cells on a flat surface in a two-dimensional format due to its simplicity. A three-dimensional (3D) format can provide a microenvironment for surrounding cells. There are two main techniques for obtaining 3D structures based on the presence of scaffolding. Scaffold-free techniques consist of spheroid technologies. Meanwhile, scaffold techniques contain organoids and all constructs that use various types of scaffolds, ranging from decellularized extracellular matrix (dECM) through hydrogels that are one of the most extensively studied forms of potential scaffolds for 3D culture up to 4D bioprinted biomaterials. 3D bioprinting is one of the most important techniques used to create biomimetic scaffolds. The versatility of this technique allows the use of many different types of inks, mainly hydrogels, as well as cells and inorganic substances. Increasing amounts of data provide evidence of vast potential of biomimetic scaffolds usage in tissue engineering and personalized medicine, with the main area of potential application being the regeneration of skin and musculoskeletal systems. Recent papers also indicate increasing amounts of in vivo tests of products based on biomimetic scaffolds, which further strengthen the importance of this branch of tissue engineering and emphasize the need for extensive research to provide safe for humansbiomimetic tissues and organs. In this review article, we provide a review of the recent advancements in the field of biomimetic scaffolds preceded by an overview of cell culture technologies that led to the development of biomimetic scaffold techniques as the most complex type of cell culture.

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Section: 3d Cell Culturesmentioning

confidence: 99%

Biomimetic Scaffolds—A Novel Approach to Three Dimensional Cell Culture Techniques for Potential Implementation in Tissue Engineering

Górnicki,

Lambrinow,

Golkar-Narenji

et al. 2024

Nanomaterials

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“…Most of the research and clinical trials for breast cancer treatment are based on the use of tumoral cell lines and animal models for in vitro and in vivo assays, respectively [10,11]. However, primary cultures or established cell lines do not fully reflect the complex tissue architecture of the tumor, limiting their predictive value [12,13]. For in vivo experiments, cell-derived xenografts and patient-derived xenografts (PDX) are the most widely used preclinical models.…”

Section: Introductionmentioning

confidence: 99%

Breast Cancer Tissue Explants: An Approach to Develop Personalized Therapy in Public Health Services

Carranza-Rosales,

Valencia-Mercado,

Esquivel-Hernández

et al. 2023

JPM

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Breast cancer is one of the main causes of death worldwide. Lately, there is great interest in developing methods that assess individual sensitivity and/or resistance of tumors to antineoplastics to provide personalized therapy for patients. In this study we used organotypic culture of human breast tumor slices to predict the experimental effect of antineoplastics on the viability of tumoral tissue. Samples of breast tumor were taken from 27 patients with clinically advanced breast cancer; slices were obtained and incubated separately for 48 h with paclitaxel, docetaxel, epirubicin, 5-fluorouracil, cyclophosphamide, and cell culture media (control). We determined an experimental tumor sensitivity/resistance (S/R) profile by evaluating tissue viability using the Alamar Blue® metabolic test, and by structural viability (histopathological analyses, necrosis, and inflammation). These parameters were related to immunohistochemical expression of the estrogen receptor, progesterone receptor, and human epidermal growth factor receptor 2. The predominant histological type found was infiltrating ductal carcinoma (85.2%), followed by lobular carcinoma (7.4%) and mixed carcinoma (7.4%). Experimental drug resistance was related to positive hormone receptor status in 83% of samples treated with cyclophosphamide (p = 0.027). Results suggest that the tumor S/R profile can help to predict personalized therapy or optimize chemotherapeutic treatments in breast cancer.

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Organoid models of breast cancer in precision medicine and translational research

Niazi,

Parseh

2024

Mol Biol Rep

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Cancer organoids: A platform in basic and translational research

Cited by 11 publications

References 203 publications

Biomimetic Scaffolds—A Novel Approach to Three Dimensional Cell Culture Techniques for Potential Implementation in Tissue Engineering

Biomimetic Scaffolds—A Novel Approach to Three Dimensional Cell Culture Techniques for Potential Implementation in Tissue Engineering

Breast Cancer Tissue Explants: An Approach to Develop Personalized Therapy in Public Health Services

Organoid models of breast cancer in precision medicine and translational research

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