Cancers du sein triples négatifs: Une revue de la littérature

Campone, Mario; Bourbouloux, Emmanuelle; Berton‐Rigaud, Dominique; Sadot, S.; Frenel, Jean‐Sébastien; Juin, Philippe

doi:10.1007/978-2-8178-0245-9_37

Cited by 1 publication

(4 citation statements)

References 82 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Similarly, docetaxel is an antimitotic agent from the taxane family which stops cell mitosis division in proliferating tumoral cells. It has shown to be of interest for TNBL tumor treatment when used in combination with anti-angiogenic agents [28]. In our experimental conditions, cisplatin and docetaxel effectiveness analysis showed cytostatic activity only on proliferative MDA-MB-231 cell line whereas only a slight toxicity was detected on non-proliferative SUM1315 cell line model.…”

Section: Discussionmentioning

confidence: 63%

“…Indeed, cisplatin (platinum salt) is a cell cycle-dependent alkylating agent which creates inter and intrastrand DNA links, inducing serious DNA double-strand damage after the replication fork. Hence, this class of drug is promoted for BLTN tumors in combination with other chemotherapy agents [28]. Similarly, docetaxel is an antimitotic agent from the taxane family which stops cell mitosis division in proliferating tumoral cells.…”

Section: Discussionmentioning

confidence: 99%

“…Epirubicin is known to be more effective on triplenegative breast tumors [28]. This drug is a very powerful non cell-cycle dependent DNA intercalating agent from the anthracycline family.…”

Section: Discussionmentioning

confidence: 99%

“…For these experiments, three drugs used in treatment protocols for BLTN breast cancers, alone or in combination, such as cisplatin, docetaxel and epirubicin were chosen [28]. Quantitative viability tests, spheroid diameter and Live/Dead® viability tests were performed.…”

Section: Discussionmentioning

confidence: 99%

See 3 more Smart Citations

Development and cytotoxic response of two proliferative MDA-MB-231 and non-proliferative SUM1315 three-dimensional cell culture models of triple-negative basal-like breast cancer cell lines

et al. 2017

View full text Add to dashboard Cite

Triple-Negative Basal-Like tumors, representing 15 to 20% of breast cancers, are very aggressive and with poor prognosis. Targeted therapies have been developed extensively in preclinical and clinical studies to open the way for new treatment strategies. The present study has focused on developing 3D cell cultures from SUM1315 and MDA-MB-231, two triple-negative basal-like (TNBL) breast cancer cell lines, using the liquid overlay technique. Extracellular matrix concentration, cell density, proliferation, cell viability, topology and ultrastructure parameters were determined. The results showed that for both cell lines, the best conditioning regimen for compact and homogeneous spheroid formation was to use 1000 cells per well and 2% Geltrex®. This conditioning regimen highlighted two 3D cell models: non-proliferative SUM1315 spheroids and proliferative MDA-MB-231 spheroids. In both cell lines, the comparison of 2D vs 3D cell culture viability in the presence of increasing concentrations of chemotherapeutic agents i.e. cisplatin, docetaxel and epirubicin, showed that spheroids were clearly less sensitive than monolayer cell cultures. Moreover, a proliferative or non-proliferative 3D cell line property would enable determination of cytotoxic and/or cytostatic drug activity. 3D cell culture could be an excellent tool in addition to the arsenal of techniques currently used in preclinical studies.

show abstract

Section: Discussionmentioning

confidence: 63%

Section: Discussionmentioning

confidence: 99%

“…Epirubicin is known to be more effective on triplenegative breast tumors [28]. This drug is a very powerful non cell-cycle dependent DNA intercalating agent from the anthracycline family.…”

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

See 2 more Smart Citations