Candida-Associated Denture Stomatitis and Murine Models: What Is the Importance and Scientific Evidence?

Abstract

Considering the high prevalence and recurrence of Candida-associated denture stomatitis (CADS), in vivo studies in animal models are necessary before those in humans to evaluate new therapeutic strategies. This study aimed to review the literature on murine models of CADS induction using acrylic intraoral devices simulating dentures. Rats are recommended as experimental animals in these models as well as the adoption of a pasty diet. For maintenance in the proper position during the experiments, intraoral appliances must be obtained by individual impressions, using and retained exclusively by cementation on the molars. The region of interest for histopathological analysis was standardized as that corresponding to the area between the first molars. However, there is no consensus among the studies on the CADS induction rat models in relation to the Candida albicans inoculation and need for immunosuppression and/or administration of antibacterial drugs of animals. The greatest difficulty of the available models refers to maintaining the course of the lesion for a sufficient period to evaluate the effectiveness of the proposed treatment, considering the rapid and efficient murine immune response to candidal colonization. Therefore, future studies are necessary for the development of a robust and reproducible CADS model.

Study on the role of nano antibacterial materials in orthodontics (a review)

“…Microbial plaque attaches to acrylic resin appliances with a larger adhesion surface than normal teeth, which can contribute to cariogenic oral flora growth (Arab et al, 2021). Candida Albicans (CA) [Candida-induced stomatitis] is an erythematous infection (red areas) of the oral mucosa that can develop beneath retainers, detachable appliances, or dentures (denture stomatitis) (Ribeiro et al, 2006;Höfling et al, 2010;Rebolledo-Cobos and Sánchez-Molina, 2018;Sugio et al, 2020). Oral Candida carriage was identified in 25-75 percent of research groups, indicating that CA is an opportunistic infection.…”

“…It's critical to detect new ways to treat CA that have developed a resistance to antifungal medicines. Antimicrobial characteristics of NPincorporated acrylic polymers and their usage in detachable instruments are yet in the preliminary phases of research, and in-vitro models are being used (Sultan et al, 2019;Sugio et al, 2020). After adding SiO 2 (1%) and TiO 2 (0.5%) NPs to a PMMA acrylic resin, Gad et al ( 2020) investigated how the flexural strength changed.…”

A Denture Use Model Associated with Candida spp. in Immunocompetent Male and Female Rats

“…Animal models have been used to investigate the pathogenesis of oral candidiasis and therapeutic approaches [ 7 , 20 , 21 ]. Many studies have used a murine model of oral candidiasis, in which immunosuppressed female mice are inoculated with C. albicans , resulting in white lesions on the tongue [ 22 , 23 , 24 ].…”

Antimicrobial efficacy and biocompatibility of extracts from Cryptocarya species

“…One such example of oropharyngeal infection is Denture Stomatitis (DS). DS is an inflammatory condition encountered most commonly in removable denture wearers and is characterized by diffuse erythema in the areas covered by the dentures, which can present as reddened spots, different textures in the palatal mucosa surface or with a homogeneous aspect [5][6][7]. The etiology of DS is extremely variable and is considered multifactorial.…”