Candida floccosa sp. nov., a novel methanol-assimilating yeast species

Abstract

Two methanol-assimilating yeast strains were isolated from a flux of a sessile oak (Quercus petrea) in Hungary and one genetically and phenotypically very similar strain from a flux of a red oak (Quercus rubra) in Canada. The strains exhibited ascomycetous affinity but ascospore formation was not observed. On the basis of the sequence of their D1/D2 domain of the large subunit rDNA, as well as of their physiological characteristics, they represent a novel yeast species of the genus Candida. Therefore Candida floccosa sp. nov. is proposed, with NCAIM Y.01581T (=CBS 10307T=NRRL Y-27951T) as the type strain.

Ogataea populialbae sp. nov., a yeast species from white poplar

“…As in our previous surveys, the majority of the tree exudates were collected in the Pilis Mountains (Péter et al, , 2006; white poplars in and near this area were sought to decide whether O. populialbae associates with this tree or not. Ogataea populialbae was not recovered during our earlier collections in Hungary; however, exudates from P. alba were not sampled previously.…”

“…Ogataea populialbae was not recovered during our earlier collections in Hungary; however, exudates from P. alba were not sampled previously. As in our previous surveys, the majority of the tree exudates were collected in the Pilis Mountains (Péter et al, , 2006; white poplars in and near this area were sought to decide whether O. populialbae associates with this tree or not. In 2008, several fluxing P. alba trees were found on the Szentendre Island embraced by the river Danube and located just at the Eastern foot of the Pilis Mountains, about 100-110 km away from the collection sites on the Great Plain.…”

“…Insects, especially Drosophila, were found to be significant vectors of yeasts and bacteria in a temperate forest (Gilbert, 1980). In the last decade, several new methanol-assimi-lating yeast species were described based on sets of strains originating exclusively or partly from tree exudates: Ogataea (Pichia) trehaloabstinens , Ogataea falcaomoraisii (Morais et al, 2004), Ogataea (Pichia) siamensis (Limtong et al, 2004), Komagataella phaffii (Kurtzman, 2005) and Candida floccosa (Péter et al, 2006). Some strains mentioned in the original description of Candida succiphila (Lee & Komagata, 1980) were also recovered from tree saps.…”

“…Some strains mentioned in the original description of Candida succiphila (Lee & Komagata, 1980) were also recovered from tree saps. In the last decade, several new methanol-assimi-lating yeast species were described based on sets of strains originating exclusively or partly from tree exudates: Ogataea (Pichia) trehaloabstinens , Ogataea falcaomoraisii (Morais et al, 2004), Ogataea (Pichia) siamensis (Limtong et al, 2004), Komagataella phaffii (Kurtzman, 2005) and Candida floccosa (Péter et al, 2006). Dlauchy et al (2003) observed that in the case of tree exudates, the enrichment technique yielded methanol-assimilating yeast strains in several cases, where their number was otherwise too low to allow their isolation using the direct surfaceplating technique.…”

Ogataea cecidiorum sp. nov., a methanol-assimilating yeast isolated from galls on willow leaves

“…Methanol-assimilating yeasts have been observed from the various plant-related substrates, like slime fluxes (Péter et al 2006), tree bark, rotten wood material (Péter et al 2003), and leaves (Péter et al 2007). Recently they have been detected on about 45% of leaves collected in Hungary (Péter et al 2007).…”