Candida maltosa

Mauersberger, Stephan; Ohkuma, Moriya; Schunck, Wolf‐Hagen; Takagi, Masamichi

doi:10.1007/978-3-642-79856-6_12

Cited by 45 publications

(48 citation statements)

References 360 publications

(729 reference statements)

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“…Yeast strains capable of assimilating hydrocarbons have been isolated from microbial communities in polluted soil or water and were generally described as biodegrading mostly n-alkanes with long carbon chains C 10 -C 24 (Mauersberger, 1996). Biosurfactants produced by strains, particularly Candida and Yarrowia, growing on hydrocarbon substrates were studied and were found to be sophorolipids, mannosylerythritol lipids, carbohydrateprotein-lipid complexes, carbohydrate-protein complexes, or fatty acids (Van Bogaert et al, 2007;Amaral et al, 2008;Campos-Takaki et al, 2010).…”

Section: Introductionmentioning

confidence: 99%

Phylogenetic analysis and characterization of an alkane-degrading yeast strain isolated from oil-polluted soil

Csutak¹,

Vassu²

2014

Turk J Biol

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IntroductionThe yeast strains belonging to the Candida, Pichia, Rhodotorula, and Yarrowia genera are successfully used in biotechnology domains related to the food and chemical industries, therapeutics, and bioremediation. The process of bioremediation is based on the ability of microorganisms to assimilate and biodegrade petroleum or crude oil by using the hydrocarbon compounds as main carbon sources in specific metabolic pathways (Vidali, 2001). The final products of this metabolism are sometimes represented by biosurfactants, surface-active molecules produced on the surface of cells whose diverse structure allows them to reduce surface and interfacial tension and to mediate the assimilation of hydrophobic substrates, including hydrocarbons, at the surface of cells (Muthusamy et al., 2008;Kaya et al., 2014). Therefore, the development of knowledge concerning the structure and production of biosurfactants is important for the improvement of bioremediation technologies in oil recovery, agriculture, cosmetics, therapeutics, and microbial lipase production

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Section: Introductionmentioning

confidence: 99%

Phylogenetic analysis and characterization of an alkane-degrading yeast strain isolated from oil-polluted soil

Csutak¹,

Vassu²

2014

Turk J Biol

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“…The Y. lipolytica strains used in this study are listed in Table 1. They were grown at 28°C in complete media, YPD (3), and YNBcas (YNBD with 0.2% Casamino Acids) (35) or in minimal media derived from YNB (35) or M (a slightly modified YNB medium) (17) containing the following carbon sources: glucose (1 or 2%; YNBD), oleic acid (1% in 0.05% Tween 80, added as 20-fold sonicated stock emulsion; YNBO), tributyrin (1% in 0.05% Tween 80, added as 20-fold sonicated emulsion; YNBT), alkanes (1 or 2%) of different chain lengths (YNBC10, decane; YNBC12, dodecane; YNBC16, hexadecane). For solid media, 20 g of agar per liter was added.…”

Section: Methodsmentioning

confidence: 99%

“…Based on the relative frequencies of alkane and auxotrophic mutants, 80 to 90 genes appeared to be involved in n-alkane assimilation. The Alk Ϫ mutants were classified into five phenotypic classes (alkA to alkE) depending on their use of intermediates of the alkane degradation pathway (fatty alcohol, fatty aldehyde, fatty acid, and acetate) (5,6,14,17). Among at least 26 loci affecting uptake and primary alkane oxidation, 16 were required for efficient n-alkane uptake (6).…”

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confidence: 99%

“…Among at least 26 loci affecting uptake and primary alkane oxidation, 16 were required for efficient n-alkane uptake (6). Mauersberger et al isolated Alk Ϫ mutants of Y. lipolytica and Candida maltosa using C 10 , C 12 , and C 16 as substrates and subdivided alkA mutants into alkAa (unable to use any n-alkane), alkAb (no growth on C 8 to C 10 ), and alkAc (no growth on C 16 ) (17). This, and studies on cytochrome P450 regulation in Y. lipolytica and C. maltosa, suggested the existence of several chain length-specific alkane uptake or cytochrome P450 monooxygenase systems catalyzing the primary terminal hydroxylation (17).…”

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confidence: 99%

“…Mauersberger et al isolated Alk Ϫ mutants of Y. lipolytica and Candida maltosa using C 10 , C 12 , and C 16 as substrates and subdivided alkA mutants into alkAa (unable to use any n-alkane), alkAb (no growth on C 8 to C 10 ), and alkAc (no growth on C 16 ) (17). This, and studies on cytochrome P450 regulation in Y. lipolytica and C. maltosa, suggested the existence of several chain length-specific alkane uptake or cytochrome P450 monooxygenase systems catalyzing the primary terminal hydroxylation (17). This was proven for C. maltosa (23,37) and more recently for Y. lipolytica (12), by identifying up to eight cytochrome P450 genes (ALK1 to ALK8) in this species, all belonging to the CYP52 gene family.…”

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confidence: 99%

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Insertional Mutagenesis in the n -Alkane-Assimilating Yeast Yarrowia lipolytica : Generation of Tagged Mutations in Genes Involved in Hydrophobic Substrate Utilization

et al. 2001

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Tagged mutants affected in the degradation of hydrophobic compounds (HC) were generated by insertion of a zeta-URA3 mutagenesis cassette (MTC) into the genome of a zeta-free and ura3 deletion-containing strain of Yarrowia lipolytica. MTC integration occurred predominantly at random by nonhomologous recombination. A total of 8,600 Ura ؉ transformants were tested by replica plating for (i) growth on minimal media with alkanes of different chain lengths (decane, dodecane, and hexadecane), oleic acid, tributyrin, or ethanol as the C source and (ii) colonial defects on different glucose-containing media (YPD, YNBD, and YNBcas). A total of 257 mutants were obtained, of which about 70 were affected in HC degradation, representing different types of non-alkane-utilizing (Alk ؊ ) mutants (phenotypic classes alkA to alkE) and tributyrin degradation mutants. Among Alk ؊ mutants, growth defects depending on the alkane chain length were observed (alkAa to alkAc). Furthermore, mutants defective in yeast-hypha transition and ethanol utilization and selected auxotrophic mutants were isolated. Flanking borders of the integrated MTC were sequenced to identify the disrupted genes. Sequence analysis indicated that the MTC was integrated in the LEU1 locus in N083, a leucine-auxotrophic mutant, in the isocitrate dehydrogenase gene of N156 (alkE leaky), in the thioredoxin reductase gene in N040 (alkAc), and in a peroxine gene (PEX14) in N078 (alkD). This indicates that MTC integration is a powerful tool for generating and analyzing tagged mutants in Y. lipolytica.

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Vectors for gene expression and amplification in the yeastYarrowia lipolytica

Juretzek¹,

Dall²,

Mauersberger³

et al. 2001

Yeast

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New vector systems were developed for gene expression in Y. lipolytica. These plasmids contain: (a) as integration target sequences, either a rDNA region or the long terminal repeat zeta of the Y. lipolytica retrotransposon Ylt1; (b) the YlURA3 gene as selection marker for Y. lipolytica, either as the non‐defective ura3d1 allele for single integration or the promotor truncated ura3d4 allele for multiple integration; (c) the inducible ICL1 or XPR2 promoters for gene expression; and (d) unique restriction sites for gene insertion. Multiple plasmid integration occurred as inserted tandem‐repeats, which are present at 3–39 copies per cell. A correlation between gene copy number and the expressed enzyme activity was demonstrated with Escherichia coli lacZ as reporter gene under the control of the regulated ICL1 promoter. Increases in copy numbers from 5 to 13 for the lacZ expression cassettes resulted in an up to 10–11‐fold linear increase of the β‐galactosidase activity in multicopy transformants during their growth on ethanol or glucose, compared with the low‐copy replicative plasmid transformants (1.6 plasmid copies). These new tools will enhance the interest in Y. lipolytica as an alternative host for heterologous protein production. Copyright © 2000 John Wiley & Sons, Ltd.

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Candida maltosa

Cited by 45 publications

References 360 publications

Phylogenetic analysis and characterization of an alkane-degrading yeast strain isolated from oil-polluted soil

Phylogenetic analysis and characterization of an alkane-degrading yeast strain isolated from oil-polluted soil

Insertional Mutagenesis in the n -Alkane-Assimilating Yeast Yarrowia lipolytica : Generation of Tagged Mutations in Genes Involved in Hydrophobic Substrate Utilization

Vectors for gene expression and amplification in the yeastYarrowia lipolytica

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