Candidate gene polymorphisms (BoIFNG, TLR4, SLC11A1) as risk factors for paratuberculosis infection in cattle

Pinedo, Pablo J.; Buergelt, C. D.; Donovan, G. A.; Meléndez, P.; Morel, Laurence; Wu, Rongling; Langaee, Taimour; Rae, D. O.

doi:10.1016/j.prevetmed.2009.05.020

Cited by 76 publications

(54 citation statements)

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“…Different approaches have been used to localise and identify the genes involved in susceptibility to MAP, including the testing of candidate genes [27] and QTL mapping using relatively low density markers [32]. More recently, following the publication of the bovine genome sequence, a genome wide association study (GWA) using higher density SNP panels has provided evidence of a genetic component in Jones's disease susceptibility [33].…”

Section: Discussionmentioning

confidence: 99%

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Genetic Loci Involved in Antibody Response to Mycobacterium avium ssp. paratuberculosis in Cattle

et al. 2010

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Background Mycobacterium avium subsp. paratuberculosis (MAP) causes chronic enteritis in a wide range of animal species. In cattle, MAP causes a chronic disease called Johne's disease, or paratuberculosis, that is not treatable and the efficacy of vaccine control is controversial. The clinical phase of the disease is characterised by diarrhoea, weight loss, drop in milk production and eventually death. Susceptibility to MAP infection is heritable with heritability estimates ranging from 0.06 to 0.10. There have been several studies over the last few years that have identified genetic loci putatively associated with MAP susceptibility, however, with the availability of genome-wide high density SNP maker panels it is now possible to carry out association studies that have higher precision.Methodology/Principal FindingsThe objective of the current study was to localize genes having an impact on Johne's disease susceptibility using the latest bovine genome information and a high density SNP panel (Illumina BovineSNP50 BeadChip) to perform a case/control, genome-wide association analysis. Samples from MAP case and negative controls were selected from field samples collected in 2007 and 2008 in the province of Lombardy, Italy. Cases were defined as animals serologically positive for MAP by ELISA. In total 966 samples were genotyped: 483 MAP ELISA positive and 483 ELISA negative. Samples were selected randomly among those collected from 119 farms which had at least one positive animal.Conclusion/SignificanceThe analysis of the genotype data identified several chromosomal regions associated with disease status: a region on chromosome 12 with high significance (P<5×10−6), while regions on chromosome 9, 11, and 12 had moderate significance (P<5×10−5). These results provide evidence for genetic loci involved in the humoral response to MAP. Knowledge of genetic variations related to susceptibility will facilitate the incorporation of this information into breeding programmes for the improvement of health status.

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Section: Discussionmentioning

confidence: 99%

“…Polymorphisms in functional candidate genes, SLC11A1 [27], TLR1 , 2 and 4 , [28] and CARD15 [29] have been associated with susceptibility with MAP infection in cattle. CARD15 has also been associated with increased the risk of Crohn's disease in humans [30], [31].…”

Section: Introductionmentioning

confidence: 99%

Genetic Loci Involved in Antibody Response to Mycobacterium avium ssp. paratuberculosis in Cattle

et al. 2010

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“…A genome wide linkage analysis identified a quantitative trait locus associated with MAP susceptibility on Bos taurus chromosome 20 (BTA 20) (Gonda et al, 2007). Polymorphisms with significant associations to MAP susceptibility were found in the following candidate genes: CARD15 (Pinedo et al, 2009a;Ruiz-Larranaga et al, 2010a), IL10 , TLR1, TLR2, TLR4 (Mucha et al, 2009;Purdie et al, 2011) and SLC11A1 (Pinedo et al, 2009b;Ruiz-Larranaga et al, 2010b). The first genome-wide association (GWA) study using the Illumina Bovine SNP50 BeadChip related to MAP susceptibility was published by Settles et al (2009).…”

Section: Introductionmentioning

confidence: 99%

Phenotype definition is a main point in genome-wide association studies for bovine Mycobacterium avium ssp. paratuberculosis infection status

Küpper

Brandt

Donat³

et al. 2014

Animal

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Paratuberculosis caused by Mycobacterium avium ssp. paratuberculosis (MAP) causes economic losses and is present in dairy herds worldwide. Different studies used different diagnostic tests to detect infection status and are the basis of genome-wide association (GWA) studies with inconsistent results. Therefore, the aim of this study was to identify and compare genomic regions associated with MAP susceptibility in the same cohort of cattle using different diagnostic tests. The GWA study was performed in German Holsteins within a case-control assay using 305 cows tested for MAP by fecal culture and additional with four different commercial ELISA-tests. Genotyping was performed with the Illumina Bovine SNP50 BeadChip. The results using fecal culture or ELISA test led to the identification of different genetic loci. Two single-nucleotide polymorphisms showed significant association with the ELISA-status. However, no significant association for MAP infection could be confirmed. Our results show that the definition of the MAP-phenotype has an important impact on the outcome of GWA studies for paratuberculosis.Keywords: cattle, paratuberculosis, genome wide association, fecal culture, ELISA Implications Paratuberculosis, caused by the agent Mycobacterium avium ssp. paratuberculosis (MAP), occurs worldwide and leads to high economic losses, due to reduced milk production, reduced fertility and higher management costs in dairy farms. Heritabilities for the susceptibility to MAP infection range between 0.031 and 0.283, whereas fecal culture shows a higher genetic background than ELISA test results. Due to different test systems used for phenotypic characterization of the MAP status of the cattle, so far association studies lead to inconsistent results. Therefore it was the aim of this study to perform a case-control genome-wide association study with MAP fecal culture phenotyped German Holstein cows which were simultaneous tested with four different ELISA tests to contribute to the understanding of genetic variability involved in MAP susceptibility. IntroductionParatuberculosis or Johne's disease is a chronic intestinal disease especially in ruminants caused by the agent Mycobacterium avium ssp. paratuberculosis (MAP). Infection with MAP occurs worldwide and leads to economic losses due to lower milk production, reduced fertility and costs associated with the management of infected animals (McKenna et al., 2006). Estimated prevalence rates for MAP are among European cattle up to 20%, but at least 3% to 5% in some countries (Nielsen and Toft, 2009). Due to a lack of country wide testing schemes, the long incubation time, subclinical phases (Chiodini et al., 1984) and problems with test method accuracy (Kohl et al., 2012), it is difficult to make a statement about prevalence rates. In 2007, the US Department of Agriculture's National Animal Health Monitoring System reported, that 68% of dairy herds in the United States were found to be infected with MAP, whereas 21.6% of these farms had at least 10% of cows infected with MA...

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“…In particular, the findings were similar to the results reported by Vásquez-Flores et al (2006), who observed banding patterns between 224 bp in an evaluation of 16 bovine breeds in Mexico (10 B. Taurus and 6 B. indicus). Pinedo et al (2009) reported bands ranging from 273 to 289 bp in a study of the associations between SLC11A1 and paratuberculosis resistance. Kadarmideen et al (2011) detected banding patterns between 211 and 217 bp for African zebu.…”

Section: Resultsmentioning

confidence: 99%

A Preliminary Study of Solute Carrier Family Gene in Adapted Bovine Breeds of Panama

Cortés¹,

Herrera²

2015

Italian Journal of Animal Science

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SLC11A1, the solute carrier family gene formerly known as bovine natural resistanceassociated macrophage protein (NRAMP), has the polymorphism of the associated microsatellites located in its 3' untranslated region (UTR). SLC11A1 has been associated with susceptibility or resistance to several intracellular pathogens. In Panama, several genetic characterization studies have been performed on the Guaymí (GY) and Guabalá (GUA) Creole breeds. Given that these breeds adapted to the local environment over a long period, there is an extremely high probability that an examination of the SLC11A1 gene could reveal alleles of this gene that confer resistance to disease. Therefore, the aim of this study was to identify the associated microsatellites located in its 3' UTR of the SLC11A1 gene in bovine breeds locally adapted to Panama. Four locally adapted bovine breeds were studied. In total, 35 of the amplified samples were sequenced, revealing new polymorphisms such as (GT)3, (GT)4 and (GT)5 in GT regions. The most common repeats among the evaluated populations were (GT)11, (GT)6 and (GT)4, which were found in 34.3, 20.0 and 11.4% of samples, respectively. Moreover, the (GT)13 repeat, which is associated with natural resistance against brucellosis, was found in the GUA breed. In addition, based on the numbers of GT repeats found in the sequenced samples, the GUA breed exhibited the greatest number of polymorphisms among the examined breeds.

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Candidate gene polymorphisms (BoIFNG, TLR4, SLC11A1) as risk factors for paratuberculosis infection in cattle

Cited by 76 publications

References 33 publications

Genetic Loci Involved in Antibody Response to Mycobacterium avium ssp. paratuberculosis in Cattle

Genetic Loci Involved in Antibody Response to Mycobacterium avium ssp. paratuberculosis in Cattle

Phenotype definition is a main point in genome-wide association studies for bovine Mycobacterium avium ssp. paratuberculosis infection status

A Preliminary Study of Solute Carrier Family Gene in Adapted Bovine Breeds of Panama

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