Candidate Genes and Pathways in Rice Co-Responding to Drought and Salt Identified by gcHap Network

Hao, Zhiqi; Ma, Stefan; Liang, Lunping; Tian, Feng; Xiong, Mei; Lian, Shangshu; Zhu, Jingyan; Chen, Yanjun; Meng, Liang; Li, Min

doi:10.3390/ijms23074016

Cited by 6 publications

(6 citation statements)

References 104 publications

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“…Proline accumulation in leaves may be involved in one or more of the above processes and contribute to drought tolerance (Ghafoor et al 2019). The rate-limiting step in proline synthesis is controlled by the bifunctional P5CS enzyme, which is encoded by two highly homologous genes in Arabidopsis plants and many other plan (Amini et al 2015;Hao et al 2022) The use of drought-resistant rice varieties is considered as the easiest and cheapest way to deal with drought stress (Temesgen 2021). Each rice variety has different biotic and abiotic stress resistance which is in uenced by the genes contained in respective varieties.…”

Section: Discussionmentioning

confidence: 99%

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Improving Drought Resistance by Introduction of the Proline Synthesis Gene P5CSA into Inbred Rice Lines as a New Genetic Resource for Breeding Programs

Herawati

Masdar

Sutrawati

et al. 2023

Preprint

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This research was aimed to identifying the P5CS gene involved in the drought stress mechanism in upland rice lines which is candidate as new genetic resource for breeding programs. The plant material consisted of 19 breed lines: Salumpikit and IR20 varieties, drought-tolerant and sensitive, respectively. The experiment consisted of 4 stages, including the evaluation of drought stress with 20% PEG 6000 (-0.58 MPa) in the germination and nursery phases, the vegetative phase, and the expression analysis of the P5CS gene. The results showed that the PEG inhibited the growth of roots, shoots, and the ratio of roots to shoot in the germination and nursery phases of all the tested lines, while the Salumpikit and IR20 varieties were confirmed as drought resistant and sensitive, respectively. The proline content under drought stress was significantly different in the lines tested, while Salumpikit and IR20 were confirmed to have high and low proline content, respectively. The proline content in several lines, such as G4, G6, G8, G10, G12, G13, G14, G15, and G17, exceeded the content in the Salumpikit variety. The P5CS gene was amplified in PCR analysis and expressed in the consistency of proline. It was found that the lines of G4, G6, G8, G13, and G17 showed tolerance to drought stress, had high STI values, and showed recovery ability and proline content. These lines have the potential to be released as candidates for new varieties. In addition, these lines have great potential as a new genetic source for upland rice breeding programs.

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Section: Discussionmentioning

confidence: 99%

“…At the molecular level. drought stress can change gene expression, and enzymatic synthesis (Hao et al 2022).…”

Section: Introductionmentioning

confidence: 99%

Improving Drought Resistance by Introduction of the Proline Synthesis Gene P5CSA into Inbred Rice Lines as a New Genetic Resource for Breeding Programs

Herawati

Masdar

Sutrawati

et al. 2023

Preprint

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“…In addition to the pathways listed above, the majority of DEGs in the salt-tolerant cultivar HD961 that responded to SS through transcriptional activation is involved in pathways such as alpha-linolenic acid metabolism, plant hormone signal transduction, plant-pathogen interaction, fatty acid elongation, MAPK signaling pathway–plant, glutathione metabolism, and diterpenoid biosynthesis. Of these pathways, plant hormone signal transduction, MAPK signaling pathway–plant, and glutathione metabolism have been a focus of research on rice undergoing abiotic stress ( Kong et al, 2019 ; Hao et al, 2022 ).…”

Section: Discussionmentioning

confidence: 99%

Combined transcriptome and metabolome reveal glutathione metabolism plays a critical role in resistance to salinity in rice landraces HD961

et al. 2022

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Rice (Oryza sativa) is one of the most important food crops around the world, which is sensitive to salt stress, especially in the seedling and booting stage. HD961 is a salt-tolerant rice landrace that grows along coastal beaches and has disease and insect pest resistance, salt tolerance, and vigorous growth characteristics. We performed a combined transcriptome and metabolome analysis to clarify salinity resistance mechanisms in cultivar HD961, which has adapted to salinity soil at the early seedling stage. The results showed that the growth and antioxidant capacity of HD961 were stronger than 9311 under salt stress (SS). Transcriptomic analysis showed that a total of 6,145, 3,309, 1,819, and 1,296 differentially expressed genes (DEGs) were identified in the groups of TH60 (control group vs. 60 mM group of HD961 for transcriptome), TH120 (control group vs. 120 mM group of HD961 for transcriptome), T60 (control group vs. 60 mM group of 9311 for transcriptome), and T120 (control group vs. 120 mM group of 9311 for transcriptome), respectively. Starch and sucrose metabolism and phenylpropanoid biosynthesis were shared in the four treatment groups based on a KEGG enrichment analysis of DEGs. In addition, alpha-linolenic acid metabolism, plant hormone signal transduction, plant-pathogen interaction, and fatty acid elongation were specific and significantly different in HD961. A total of 92, 158, 151, and 179 significantly regulated metabolites (SRMs) responded to SS in MH60 (control group vs. 60 mM group of HD961 for metabolome), MH120 (control group vs. 120 mM group of HD961 for metabolome), M60 (control group vs. 60 mM group of 9311 for metabolome), and M120 (control group vs. 120 mM group of 9311 for metabolome), respectively. The KEGG analysis showed that eight common metabolic pathways were identified in the four treatment groups, of which biosynthesis of amino acids was the most significant. Three specific metabolic pathways were identified in the HD961, including glutathione metabolism, ascorbate and aldarate metabolism, and pantothenate and CoA biosynthesis. Integrative analysis between the transcriptome and metabolome showed that glutathione metabolism was specific and significantly affected under SS in HD961. A total of seven SRMs and 48 DEGs and four SRMs and 15 DEGs were identified in the glutathione metabolism pathway in HD961 and 9311, respectively. The Pearson correlation analysis showed a significant correlation between reduced glutathione and 16 genes (12 upregulated and four downregulated genes), suggesting these genes could be candidates as salt-tolerance regulation genes. Collectively, our data show that glutathione metabolism plays a critical role in response to SS in rice. Moreover, the stronger regulative ability of related common genes and metabolites might contribute to salt resistance in HD961.

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“…Most drought and salt stress studies focus on roots and shoots, with measurements of physiological and genetic parameters ( Qin et al., 2020 ; Hao et al., 2022 ). Among them, ABA plays an important role in plant responses to abiotic stresses ( Zhao et al., 2021 ).…”

Section: Physiological and Molecular Implications Of Combined Abiotic...mentioning

confidence: 99%

Physiological and molecular implications of multiple abiotic stresses on yield and quality of rice

et al. 2023

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Abiotic stresses adversely affect rice yield and productivity, especially under the changing climatic scenario. Exposure to multiple abiotic stresses acting together aggravates these effects. The projected increase in global temperatures, rainfall variability, and salinity will increase the frequency and intensity of multiple abiotic stresses. These abiotic stresses affect paddy physiology and deteriorate grain quality, especially milling quality and cooking characteristics. Understanding the molecular and physiological mechanisms behind grain quality reduction under multiple abiotic stresses is needed to breed cultivars that can tolerate multiple abiotic stresses. This review summarizes the combined effect of various stresses on rice physiology, focusing on grain quality parameters and yield traits, and discusses strategies for improving grain quality parameters using high-throughput phenotyping with omics approaches.

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Candidate Genes and Pathways in Rice Co-Responding to Drought and Salt Identified by gcHap Network

Cited by 6 publications

References 104 publications

Improving Drought Resistance by Introduction of the Proline Synthesis Gene P5CSA into Inbred Rice Lines as a New Genetic Resource for Breeding Programs

Improving Drought Resistance by Introduction of the Proline Synthesis Gene P5CSA into Inbred Rice Lines as a New Genetic Resource for Breeding Programs

Combined transcriptome and metabolome reveal glutathione metabolism plays a critical role in resistance to salinity in rice landraces HD961

Physiological and molecular implications of multiple abiotic stresses on yield and quality of rice

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