Candidate Polyurethanes Based on Castor Oil (Ricinus communis), with Polycaprolactone Diol and Chitosan Additions, for Use in Biomedical Applications

Abstract

Polyurethanes are widely used in the development of medical devices due to their biocompatibility, degradability, non-toxicity and chemical versatility. Polyurethanes were obtained from polyols derived from castor oil, and isophorone diisocyanate, with the incorporation of polycaprolactone-diol (15% w/w) and chitosan (3% w/w). The objective of this research was to evaluate the effect of the type of polyol and the incorporation of polycaprolactone-diol and chitosan on the mechanical and biological properties of the polyurethanes to identify the optimal ones for applications such as wound dressings or tissue engineering. Polyurethanes were characterized by stress-strain, contact angle by sessile drop method, thermogravimetric analysis, differential scanning calorimetry, water uptake and in vitro degradation by enzymatic processes. In vitro biological properties were evaluated by a 24 h cytotoxicity test using the colorimetric assay MTT and the LIVE/DEAD kit with cell line L-929 (mouse embryonic fibroblasts). In vitro evaluation of the possible inflammatory effect of polyurethane-based materials was evaluated by means of the expression of anti-inflammatory and proinflammatory cytokines expressed in a cellular model such as THP-1 cells by means of the MILLIPLEX® MAP kit. The modification of polyols derived from castor oil increases the mechanical properties of interest for a wide range of applications. The polyurethanes evaluated did not generate a cytotoxic effect on the evaluated cell line. The assessed polyurethanes are suggested as possible candidate biomaterials for wound dressings due to their improved mechanical properties and biocompatibility.

Processing of Polyester-Urethane Filament and Characterization of FFF 3D Printed Elastic Porous Structures with Potential in Cancellous Bone Tissue Engineering

“…Consequently, the TPU_P can be pre-defined as biocompatible and non-toxic [ 33 ]. The obtained results are in common with the in vitro cytotoxicity studies on polyurethanes materials, performed by Calvo-Correas et al or Uscátegui et al [ 34 , 35 ]. Thus, the statement, common in the literature, that polyurethanes are widely used in medical applications due to their exceptional biocompatibility and non-toxicity was confirmed [ 36 ].…”

Biofuel Production with Castor Bean: A Win–Win Strategy for Marginal Land

“…Because of its high viscosity, it is used as a lubricant in two-stroke engines, neat or blended, reducing smoke emissions by up to 50-70% [113,114]. It is a polyol that can readily form polymers making polyurethanes that find applications in adhesives and coatings, electrical insulators, and semirigid foams used in thermal insulation [115]; it was also suggested as a possible candidate biomaterial for wound dressings [116] and as a graft for bone defect treatments [117]. The so-called Turkey red oil, produced by CB oil sulfation, is widely used in textile industries in dyeing and in finishing cotton and linen [118].…”

Monitoramento de artigos sobre a utilização de nanocompósitos com matriz de poliuretano aplicados à engenharia de tecidos para o reparo ósseo

“…Os PU´s possuem diversas aplicações como em membrana de separação de gases, revestimentos de superfície, materiais de engenharia de tecidos e na área biomédica (Salgado et al, 2020). Na área biomédica e engenharia de tecidos os poliuretanos biodegradáveis são aplicados na fabricação de arcabouço na regeneração óssea, devido a sua hemocompatibilidade, biocompatibilidade, degradabilidade e não toxicidade e devido ás várias limitações, deficiências e complicações nos procedimentos clínicos atuais relatados para transplantes autólogos e alogênicos, ocorrendo uma diminuição no fornecimento de enxertos, infecção e morbidade do doador local, cicatriz e complicações cirúrgicas como, dor crônica e sangramento (Bharadwaz & Jayasuriya, 2020;Gajbhiye et al, 2020;Jaganathan et al, 2019;Uscátegui et al, 2019).…”