Canine cell line, IPC‐366, as a good model for the study of inflammatory breast cancer

Abstract

Inflammatory breast cancer (IBC) is an aggressive type of cancer with poor survival in women. Inflammatory mammary cancer (IMC) in dogs is very similar to human IBC and it has been proposed as a good surrogate model for study the human disease. The aim was to determine if IPC-366 shared characteristics with the IBC cell line SUM149. The comparison was conducted in terms of ability to grow (adherent and nonadherent conditions), stem cell markers expression using flow cytometry, protein production using western blot and tumorigenic capacity. Our results revealed that both are capable of forming long-term mammospheres with a grape-like morphology. Adherent and nonadherent cultures exhibited fast growth in vivo. Stem cell markers expressions showed that IPC-366 and SUM149 in adherent and nonadherent conditions has mesenchymal-like characteristics, E-cadherin and N-cadherin, was higher in adherent than in nonadherent cultures. Therefore, this study determines that both cell lines are similar and IPC-366 is a good model for the human and canine disease.

Real-Time Molecular Diagnosis of Tumors Using Water-Assisted Laser Desorption/Ionization Mass Spectrometry Technology

“…Veterinary canine patients-similarly to humans-develop spontaneous tumors that share many features similar to the human disease (Takashima-Uebelhoer et al, 2012). Moreover, oncological surgery is a frontline treatment for both human (Abramson, 2010;Carpenter et al, 1991;Diamond et al, 1999;Greenfield et al, 1995;Lewis, 1995;Misdorp and Hart, 1976;Pluhar et al, 2000;Schneider, 1970;Schwyn et al, 1998) and veterinary patients (Caceres et al, 2016;Liu et al, 2011;Pena et al, 2014). Specifically, soft tissue sarcomas and osteosarcoma in dogs have similar morphological appearances, clinicopathological presentation, and phenotypes as in humans and have been shown to be an excellent comparative model.…”

Vasculogenic mimicry-associated ultrastructural findings in human and canine inflammatory breast cancer cell lines

“…VEGF-A is an angiogenic marker that is overexpressed in IBC/IMC and it is present in normal endothelial cells but also in neoplastic cells [10, 12]. According to our previous study, both cell lines overexpress VEGF-A and contributes to the exacerbated angiogenesis [15]. There is an intensive research going on in order to find effective anti-angiogenesis drugs, and more than 300 angiogenesis inhibitors have been identified [16].…”

“…Several human IBC cell lines such as SUM149, have been established in order to study the in vitro mechanisms of this special type of breast cancer [23, 24]. Similarly, the IPC-366 is the unique canine IMC cell line established [25] and has demonstrated to be a good model in comparison with its human counterpart SUM149 [15]. Human SUM149 and canine IPC-366 are triple negative (ER-, PR-, HER2-) epithelial cell lines, with high rates of cell growth in adherent (2D) and non-adherent (3D) conditions and metastatic capacity in mice models [15].…”

“…Similarly, the IPC-366 is the unique canine IMC cell line established [25] and has demonstrated to be a good model in comparison with its human counterpart SUM149 [15]. Human SUM149 and canine IPC-366 are triple negative (ER-, PR-, HER2-) epithelial cell lines, with high rates of cell growth in adherent (2D) and non-adherent (3D) conditions and metastatic capacity in mice models [15]. The expression of CD146, a marker of endothelial lineage stem cells, has been related in both cell lines to the presence of VM, due to the existence of CD146 positive endothelial-like cells lining the newly-formed VM channels [15].…”

“…Human SUM149 and canine IPC-366 are triple negative (ER-, PR-, HER2-) epithelial cell lines, with high rates of cell growth in adherent (2D) and non-adherent (3D) conditions and metastatic capacity in mice models [15]. The expression of CD146, a marker of endothelial lineage stem cells, has been related in both cell lines to the presence of VM, due to the existence of CD146 positive endothelial-like cells lining the newly-formed VM channels [15]. Nevertheless, according to some authors, these VM cells could not express endothelial cell markers [18, 20].…”