Canine Cutaneous Mast Cell Tumor: Morphologic Grading and Survival Time in 83 Dogs

Patnaik, A. K.; Ehler, William J.; MacEwen, E G

doi:10.1177/030098588402100503

Cited by 687 publications

(1,174 citation statements)

References 5 publications

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“…To formulate a histologic diagnosis, samples were fixed in 10% buffered formalin, processed, and embedded in paraffin using a standardized protocol. Slides were reviewed by a single board‐certified pathologist (SDP), and histopathologic criteria for diagnosis were based on those previously published for canine MCT 19, 20…”

Section: Methodsmentioning

confidence: 99%

Concordance of c‐kit Mutational Status in Matched Primary and Metastatic Cutaneous Canine Mast Cell Tumors at Baseline

Marconato

Zorzan

Giantin

et al. 2013

Veterinary Internal Medicne

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BackgroundMutation analysis of proto‐oncogene c‐kit (c‐kit) is advisable before starting treatment with tyrosine kinase inhibitors in dogs with mast cell tumor (MCT), including those with metastatic disease. Testing is usually performed on primary tumors, assuming that c‐kit mutation status does not change in metastasis.Hypothesis/ObjectivesTo give an insight into the mutational processes and to make a recommendation on the use of c‐kit mutational analysis in the clinical setting.AnimalsTwenty‐one client‐owned dogs with metastatic MCT.MethodsDogs undergoing resection or biopsy for both primary and matched metastatic MCT were prospectively enrolled. Total RNA or DNA was extracted from primary MCT and corresponding metastases. Exons 8, 9, and 11 were amplified by PCR and sequenced. Genetic features between primary MCT and metastases were compared. Their correlation with clinicopathologic features was investigated.ResultsConcordance (mutated or wild‐type) of mutational status, evaluable in 21 primary and matched metastatic (20 nodal and 1 splenic) MCTs, was 100%. Three new c‐kit mutations were identified. No significant correlation was detected between c‐kit mutation and clinicopathologic features.Conclusions and Clinical ImportanceProto‐oncogene c‐kit mutational status is conserved between any primary and its matched secondary tumor, suggesting that both can be used for c‐kit mutational testing. Targeted therapies might be also used to treat metastatic disease.

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Section: Methodsmentioning

confidence: 99%

Concordance of c‐kit Mutational Status in Matched Primary and Metastatic Cutaneous Canine Mast Cell Tumors at Baseline

Marconato

Zorzan

Giantin

et al. 2013

Veterinary Internal Medicne

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“…These tumours are routinely graded for malignancy, based on histomorphological characteristics (Patnaik et al 1984). However, the typical intracytoplasmic granules of mast cells are more difϐicult to identify using histology and histochemical stains (Giemsa or toluidine blue) in tumours with intermediate differentiation (grade II) and poor differentiation (grade III), which often makes the diagnosis more difϐicult (Patnaik et al 1984). Recently, CD117 antibody, or c-KIT, has been used as an immunohistochemical speciϐic marker for mast cells, supporting the diagnosis of less differentiated MCTs (Reguera et al 2000).…”

Section: Introductionmentioning

confidence: 99%

Histomorphological and immunohistochemical characterization of 172 cutaneous round cell tumours in dogs

Araújo

Preis

Lavalle³

et al. 2012

Pesq. Vet. Bras.

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Pesq. Vet. , cytokeratin, vimentin and E-cadherin) on formalin-ϐixed, parafϐin-embedded sections of canine cutaneous round cell tumours. Neoplastic tumours were diagnosed by histology and histochemical stains and included 107 mast cell tumours, 31 cutaneous histiocytomas, two localized histiocytic sarcomas, 21 cutaneous lymphomas, three plasma cell tumours, one transmissible venereal tumour and seven unclassiϐied round cell tumours. The histologic diagnosis was modiϐied in 39.5% of the total 172 neoplasms. The staining for CD45 and Ecadherin were variable, and therefore, the ϐinal diagnoses of cutaneous histiocytoma and localized histiocytic sarcoma were made based on histology in association with negative results for CD3, CD79a, CD117 and cytokeratin. The cellular origin of unclassiϐied round cell tumours was deϐined in all cases. Cutaneous B-cell lymphoma and plasma cell tumours were CD79a-positive and could be distinguished from each other by the morphological characteristics. Mast cell tumours and T cell lymphoma were CD117 and CD3 positive, respectively. The positive staining for vimentin and the negative staining for CD3, CD79a, CD117 and cytokeratin favoured the diagnosis of transmissible venereal tumours. Thus, the ϐinal diagnosis of cutaneous round cell tumours should be based on the interpretation of immunohistochemical results together with the cellular morphology observed by histology. Therefore, more studies to optimize the speciϐic markers in formalin-ϐixed, parafϐin-embedded tissues (especially for histiocytes) are required for deϐinitive diagnosis of round cell tumours in dogs. las redondas em cães. Os tumores foram diagnosticados usando-se a histopatologia e a marcação imuno-histoquí-mica. Foram incluídos 107 mastocitomas, 31 histiocitomas cutâneos, 2 sarcomas histiocíticos localizados, 21 linfomas cutâneos, 3 plasmocitomas, 1 tumor venéreo transmissí-vel e 7 tumores de células redondas não classiϐicados. O diagnóstico histológico foi modiϐicado em 39,5% do total de 172 neoplasias. A marcação do anticorpo CD45 e E--caderina foi variável e, nesse sentido, o diagnóstico ϐinal de histiocitoma cutâneo e sarcoma histiocítico localizado foi baseado na histologia em associação com os resultados negativos para CD3, CD79a, CD117 e citoqueratina. A origem celular dos tumores de células redondas não classiϐicados foi deϐinida em todos os casos. foram distinguidos entre si pelas características morfológi-cas. Marcação positiva para vimentina e negativa para CD3, CD79a, CD117 e citoqueratina favoreceram o diagnóstico dos tumores venereos transmissíveis. Assim, o diagnósti-co ϐinal dos tumores de células redondas foram baseados na interpretação dos resultados da imuno-histoquímica em conjunto com a avaliação das características morfoló-gicas observadas na histologia. Finalmente, mais estudos em relação à padronização de marcadores especíϐicos para tecidos paraϐinizados (especialmente para histiócitos) são necessários para o diagnóstico deϐinitivo das neoplasias de células redondas em cães.TERMOS DE INDEXAÇÃO...

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“…The initial diagnosis of cutaneous MCT was confirmed independently by two pathologists and classified according to the established histological grading system (Patnaik et al, 1984). Medical records were examined for each case and data recorded included dog signalment, number and location of tumors, presence or absence of lymph node metastasis, evolution time, recurrence interval, survival time, and cause of death.…”

Section: Specimensmentioning

confidence: 99%

“…Two 5 lm tissue sections were stained with hematoxylin/eosin and toluidine blue for histological examination and grading according to Patnaik et al (1984). Staining was performed according to an established protocol (Behmer et al, 1976a,b).…”

Section: Histological Analysis Of Canine Cutaneous Mast Cellsmentioning

confidence: 99%

“…The clinical and histological characteristics of cutaneous canine MCTs provide useful prognostic information. MCTs were first histologically classified as Grades 1, 2 and 3 by Patnaik et al (1984), with each grade correlated with survival. Recently, a 2-tier classification of MCTs has been proposed by Kiupel et al (2011) to more accurately predict biological course, particularly in the case of Grade 3 tumors.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

E-cadherin in canine mast cell tumors: Decreased expression and altered subcellular localization in Grade 3 tumors

Mackowiak

Gentile

Chaible

et al. 2012

The Veterinary Journal

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Canine Cutaneous Mast Cell Tumor: Morphologic Grading and Survival Time in 83 Dogs

Cited by 687 publications

References 5 publications

Concordance of c‐kit Mutational Status in Matched Primary and Metastatic Cutaneous Canine Mast Cell Tumors at Baseline

Concordance of c‐kit Mutational Status in Matched Primary and Metastatic Cutaneous Canine Mast Cell Tumors at Baseline

Histomorphological and immunohistochemical characterization of 172 cutaneous round cell tumours in dogs

E-cadherin in canine mast cell tumors: Decreased expression and altered subcellular localization in Grade 3 tumors

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