Canine Melanoma Diagnosis

Campagne, Cécile; Julé, Sophia; Alleaume, Charline; Bernex, Florence; Ezagal, J.; Château-Joubert, Sophie; Estrada, M.; Aubin-Houzelstein, Geneviève; Panthier, Jean‐Jacques; Egidy, Giorgia

doi:10.1177/0300985813490754

Cited by 14 publications

(8 citation statements)

References 31 publications

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“…RACK1 has been associated with several signaling pathways, and serves a vital function in tumorigenesis ( 22 ). RACK1 was reported to be overexpressed in multiple cancers including melanoma ( 23 ), gastric cancer ( 24 ), hepatocellular carcinoma ( 25 ) and pulmonary adenocarcinoma ( 26 ). RACK1 is thought to promote tumor invasion and metastasis.…”

Section: Discussionmentioning

confidence: 99%

High expression of RACK1 is associated with poor prognosis in patients with pancreatic ductal adenocarcinoma

et al. 2017

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Receptor for activated C kinase 1 (RACK1) is associated with certain aspects of cancer biology and signaling pathways, but its function in pancreatic ductal adenocarcinoma (PDAC) remains unknown. In the present study, 157 patients with PDAC were enrolled. RACK1 mRNA and protein expression levels were analyzed in PDAC tissues and matched adjacent noncancerous tissues by reverse transcription-quantitative polymerase chain reaction and western blotting. RACK1 expression levels in paraffin-embedded PDAC tissues were determined by immunohistochemistry. The associations between RACK1 expression and clinical data were evaluated using χ2 analysis. The relationship between RACK1 expression and the survival data of patients was analyzed using Kaplan-Meier and log rank tests. RACK1 mRNA and protein were revealed to be overexpressed in PDAC tumor tissues compared with adjacent noncancerous tissues. RACK1 expression was associated with clinical stage (P=0.001), lymph node invasion (P=0.003) and liver metastasis (P=0.001). Furthermore, patients with PDAC and high RACK1 expression demonstrated shorter overall survival times compared with patients with low RACK1 expression (P=0.002). Multivariate analysis indicated that RACK1 overexpression was an independent prognostic factor for patients with PDAC. Overexpression of RACK1 may contribute to tumor progression, and may be a potential prognostic biomarker for patients with PDAC.

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Section: Discussionmentioning

confidence: 99%

High expression of RACK1 is associated with poor prognosis in patients with pancreatic ductal adenocarcinoma

et al. 2017

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“…This study pointed at GNB2L1 , coding for RACK1, as a potentially interesting gene in melanoma characterization. Later, this scaffold protein with multiple functions has been described as a good marker for differentiating melanocytoma from melanoma in dog ( Campagne et al, 2013 ) and horse ( Campagne et al, 2012 ). More recently, Campagne et al (2017) showed that RACK1 could cooperate with NRAS Q61K mutation to accelerate melanoma onset and metastasis formation in transgenic mice.…”

Section: Future Directionsmentioning

confidence: 99%

The MeLiM Minipig: An Original Spontaneous Model to Explore Cutaneous Melanoma Genetic Basis

Bourneuf

2017

Front. Genet.

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Melanoma is the deadliest skin cancer and is a major public health concern with a growing incidence worldwide. As for other complex diseases, animal models are needed in order to better understand the mechanisms leading to pathology, identify potential biomarkers to be used in the clinics, and eventually molecular targets for therapeutic solutions. Cutaneous melanoma, arising from skin melanocytes, is mainly caused by environmental factors such as UV radiation; however a significant genetic component participates in the etiology of the disease. The pig is a recognized model for spontaneous development of melanoma with features similar to the human ones, followed by a complete regression and a vitiligo-like depigmentation. Three different pig models (MeLiM, Sinclair, and MMS-Troll) have been maintained through the last decades, and different genetic studies have evidenced a complex inheritance of the disease. As in humans, pigmentation seems to play a prominent role, notably through MC1R and MITF signaling. Conversely, cell cycle genes as CDKN2A and CDK4 have been excluded as predisposing for melanoma in MeLiM. So far, only sparse studies have focused on somatic changes occurring during oncogenesis, and have revealed major cytological changes and a potential dysfunction of the telomere maintenance system. Finally, the spontaneous tumor progression and regression occurring in these models could shed light on the interplay between endogenous retroviruses, melanomagenesis, and adaptive immune response.

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“…The expression of melanocytic markers in canine species has never been assessed in a dedicated study, despite numerous markers already being used in diagnostic environments, particularly for neoplastic lesions, such as amelanotic melanomas 21–24 …”

Section: Discussionmentioning

confidence: 99%

Canine melanocytes: Immunohistochemical expression of melanocytic markers in different somatic areas

Porcellato

Orlandi

Giudice

et al. 2023

Veterinary Dermatology

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Background: Melanoblasts originate in the neural crest from where they migrate to peripheral tissues and differentiate into melanocytes. Alteration during melanocyte development and life can cause different diseases, ranging from pigmentary disorders and decreased visual and auditory functions, to tumours such as melanoma. Location and phenotypical features of melanocytes have been characterised in different species, yet data on dogs are lacking.Objective: This study investigates the expression of melanocytic markers Melan A, PNL2, TRP1, TRP2, SOX-10 and MITF in melanocytes of selected cutaneous and mucosal surfaces of dogs. Animals: At necropsy, samples from five dogs were harvested from oral mucosa, mucocutaneous junction, eyelid, nose and haired skin (abdomen, back, pinna, head). Materials and Methods: Immunohistochemical and immunofluorescence analyses were performed to assess marker expression. Results: Results showed variable expression of melanocytic markers in different anatomical sites, particularly within epidermis of haired skin and dermal melanocytes. Melan A and SOX-10 were the most specific and sensitive melanocytic markers. PNL2 was less sensitive, while TRP1 and TRP2 were seldomly expressed by intraepidermal melanocytes in haired skin. MITF had a good sensitivity, yet the expression often was weak. Conclusions and Clinical Relevance: Our results indicate a variable expression of melanocytic markers in different sites, suggesting the presence of subpopulations of melanocytes. These preliminary results pave the way to understanding the pathogenetic mechanisms involved in degenerative melanocytic disorders and melanoma. Furthermore, the possible different expression of melanocyte markers in different anatomical sites could influence their sensitivity and specificity when used for diagnostic purposes.

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Canine Melanoma Diagnosis

Cited by 14 publications

References 31 publications

High expression of RACK1 is associated with poor prognosis in patients with pancreatic ductal adenocarcinoma

High expression of RACK1 is associated with poor prognosis in patients with pancreatic ductal adenocarcinoma

The MeLiM Minipig: An Original Spontaneous Model to Explore Cutaneous Melanoma Genetic Basis

Canine melanocytes: Immunohistochemical expression of melanocytic markers in different somatic areas

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