Canine mesenchymal stem cells from synovium have a higher chondrogenic potential than those from infrapatellar fat pad, adipose tissue, and bone marrow

Sasaki, Ayano; Mizuno, Mitsuru; Ozeki, Nobutake; Katano, Harutaka; Otabe, Koji; Tsuji, Kunikazu; Koga, Hideyuki; Mochizuki, Manabu; Sekiya, Ichiro

doi:10.1371/journal.pone.0202922

Cited by 71 publications

(82 citation statements)

References 42 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In chondrogenic differentiation, the MSC from fibrous synovia formed larger pellets than that from infrapatellar fat pads, while no significant differences were observed for the glycosaminoglycan/DNA ratio or the staining intensity for toluidine blue and collagen type II (27). Notably, the MSC from infrapatellar fat pads showed much higher potency for adipogenic differentiation (27). The current data for mouse ASF and FSF are consistent with the previous data from canine cells.…”

Section: Discussionsupporting

confidence: 88%

“…The MSC from fibrous synovia and infrapatellar fat pads, which are probably equivalent to FSF and ASF in the current study, respectively, had similar potency in colony formation and cell proliferation (27). In chondrogenic differentiation, the MSC from fibrous synovia formed larger pellets than that from infrapatellar fat pads, while no significant differences were observed for the glycosaminoglycan/DNA ratio or the staining intensity for toluidine blue and collagen type II (27). Notably, the MSC from infrapatellar fat pads showed much higher potency for adipogenic differentiation (27).…”

Section: Discussionsupporting

confidence: 58%

“…Katagiri et al reported a primary culture of cells isolated from human fibrous synovia and found that the colony number from fibrous synovial tissues was significantly greater than that from adipose ones (11). Sasaki et al harvested mesenchymal stem cells (MSC) from fibrous synovia, infrapatellar fat pads, inguinal adipose, and bone marrow tissues from six canines and compared their characteristics (27). The MSC from fibrous synovia and infrapatellar fat pads, which are probably equivalent to FSF and ASF in the current study, respectively, had similar potency in colony formation and cell proliferation (27).…”

Section: Discussionmentioning

confidence: 57%

“…Sasaki et al harvested mesenchymal stem cells (MSC) from fibrous synovia, infrapatellar fat pads, inguinal adipose, and bone marrow tissues from six canines and compared their characteristics (27). The MSC from fibrous synovia and infrapatellar fat pads, which are probably equivalent to FSF and ASF in the current study, respectively, had similar potency in colony formation and cell proliferation (27). In chondrogenic differentiation, the MSC from fibrous synovia formed larger pellets than that from infrapatellar fat pads, while no significant differences were observed for the glycosaminoglycan/DNA ratio or the staining intensity for toluidine blue and collagen type II (27).…”

Section: Discussionmentioning

confidence: 99%

“…Because adipose and fibrous synovial tissues are histologically different, fibroblasts derived from adipose synovium (adipose synovial fibroblasts; ASF) and those derived from fibrous synovium (fibrous synovial fibroblasts; FSF) may have different characteristics. A few studies have reported the difference between ASF and FSF in human and canine (11,27). However, this difference has not been investigated deeply and no reports have compared mouse ASF and FSF.…”

Section: Senescence-associated β-Galactosidase (Sa-β-gal)mentioning

confidence: 99%

See 4 more Smart Citations

Primary culture of mouse adipose and fibrous synovial fibroblasts under normoxic and hypoxic conditions

et al. 2020

View full text Add to dashboard Cite

Synovial fibroblasts have attracted considerable attention in studies of joint diseases. Although mice are useful and powerful in in vitro and in vivo experiments, primary cultures of mouse synovial fibroblasts are notoriously difficult because the mouse synovial tissues are much smaller and cell cycle arrests can be induced more easily in murine cells than in human cells. Here, we report a precise protocol for the isolation and culture of fibroblasts from mouse adipose and fibrous knee joint synovia. In both adipose and fibrous synovial fibroblasts, proliferation was decreased in addition to a higher rate of cellular senescence under normoxic conditions (20% O 2 ); however, it was maintained over 20 days with low cellular senescence under hypoxic conditions (3% O 2 ). The marker gene expression in adipose and fibrous synovial fibroblasts was not markedly altered after a 3-week culture. Both cells displayed similar potencies for chondrogenic, osteogenic, and adipogenic differentiation, and responses to a proinflammatory cytokine. The present method provides a sufficient amount of mouse synovial fibroblasts for in vitro and in vivo experiments in joint biology and the pathophysiology of osteoarthritis and rheumatoid arthritis.

show abstract

Section: Discussionsupporting

confidence: 88%

Section: Discussionsupporting

confidence: 58%

Section: Discussionmentioning

confidence: 57%

Section: Discussionmentioning

confidence: 99%

Section: Senescence-associated β-Galactosidase (Sa-β-gal)mentioning

confidence: 99%

See 3 more Smart Citations

Primary culture of mouse adipose and fibrous synovial fibroblasts under normoxic and hypoxic conditions

et al. 2020

View full text Add to dashboard Cite

show abstract

Mesenchymal stem cells for osteoarthritis: Recent advances in related cell therapy

Lin,

Huang,

Jiao

et al. 2024

Bioengineering & Transla Med

View full text Add to dashboard Cite

Osteoarthritis (OA) is a degenerative joint disease that affects the entire joint and has been a huge burden on the health care system worldwide. Although traditional therapy and targeted cartilage cell therapy have made significant progress in the treatment of OA and cartilage regeneration, there are still many problems. Mesenchymal stem cells from various tissues are the most studied cell type and have been used in preclinical and clinical studies of OA, because they are more widely available, have a greater capacity for in vitro expansion, and have anti‐inflammatory and immunomodulatory properties compared to autologous chondrocytes. This article will systematically review the latest developments in these areas. It may provide new insights for improving OA and cartilage regeneration.

show abstract

Human adipose and synovial mesenchymal stem cells improve osteoarthritis in rats by reducing chondrocyte reactive oxygen species and inhibiting inflammatory response

Liu

et al. 2022

Clinical Laboratory Analysis

View full text Add to dashboard Cite

Background We explored the therapeutic effects of Adipose‐derived mesenchymal stem cells (ADMSCs) and Synovial‐derived mesenchymal stem cells (SDMSCs) on osteoarthritis (OA). Methods SDMSCs and ADMSCs were co‐cultured with chondrocytes and stimulated with interleukin (IL)‐1β. An OA model was established on rats by intra‐articular injection with ADMSCs and SDMSCs. After 8 weeks, the joint diameter difference was detected, and histological staining was used to observe the pathological changes in cartilage tissue. Enzyme‐linked immunosorbent assay (ELISA) was used to detect the expressions of IL‐6, tumor necrosis factor (TNF)‐α and IL‐1β in joint fluid. The expressions of COL2A1, Aggrecan, Matrix metalloproteinase (MMP)‐13, SOX9, IL‐6, TNF‐α and IL‐1β were detected by qRT‐PCR and Western blotting in cartilage tissue. Reactive oxygen species (ROS) content in cells and cartilage tissues was detected by ROS kit. Results SDMSCs and ADMSCs co‐cultured with chondrocytes could reduce MMP‐13 expression, increase the expressions of COL2A1, Aggrecan and SOX9, as well as reverse the effects of IL‐1β on promoting ROS content and inflammatory factors levels. After the OA model was established, the injection of ADMSCs and SDMSCs reduced the differences in joint diameter and tissue lesions in OA rats. The OA model led to increased levels of IL‐6, TNF‐α and IL‐1β in joint fluid and cartilage tissue, while the injection of ADMSCs and SDMSCs inhibited the inflammatory factor levels in OA rats, and increased the expressions of COL2A1, Aggrecan and SOX9 in OA rats. Conclusion ADMSCs and SDMSCs improve osteoarthritis in rats by reducing chondrocyte ROS and inhibiting inflammatory response.

show abstract

Canine mesenchymal stem cells from synovium have a higher chondrogenic potential than those from infrapatellar fat pad, adipose tissue, and bone marrow

Cited by 71 publications

References 42 publications

Primary culture of mouse adipose and fibrous synovial fibroblasts under normoxic and hypoxic conditions

Primary culture of mouse adipose and fibrous synovial fibroblasts under normoxic and hypoxic conditions

Mesenchymal stem cells for osteoarthritis: Recent advances in related cell therapy

Human adipose and synovial mesenchymal stem cells improve osteoarthritis in rats by reducing chondrocyte reactive oxygen species and inhibiting inflammatory response

Contact Info

Product

Resources

About