Canine oocyte diameter in relation to meiotic competence and sperm penetration

Otoi, Takeshige; Fujii, Motoyuki; Tanaka, M.; Ooka, Andreas; Suzuki, Takahiro

doi:10.1016/s0093-691x(00)00368-x

Cited by 78 publications

(49 citation statements)

References 22 publications

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“…These COCs were placed in 35-mm Petri dishes (Falcon # 3001; Becton-Dickinson, Lincoln Park, NY, USA) containing PB1 medium [47] supplemented with 3 mg/mL BSA (Sigma, St Louis, MO, USA) and 100 lg/mL streptomycin (Meiji, Tokyo, Japan), and examined under a dissecting microscope (SMZ1500; Nikon Instech, Tokyo, Japan). After three washes in the same medium, the COCs were selected according to the criteria previously described by De los Reyes et al [18], Hewitt and England [48], and Otoi et al [49] under an inverted microscope (DMIR/E, Leica, Wetzlar, Germany). The conditions were those reported to favor meiotic competence based on the uniformity of ooplasm and cumulus cell complement, homogeneous dark cytoplasm with three or more layers of compact cumulus cells, and oocytes [110 lm in diameter.…”

Section: Methodsmentioning

confidence: 99%

Effect of bovine cumulus–oocyte complexes‐conditioned medium on in‐vitro maturation of canine oocytes

Abdel-Ghani

Abe

Asano

et al. 2010

Reprod Medicine & Biology

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Purpose To investigate the ability of medium conditioned with bovine cumulus-oocyte complexes (COCs) to support nuclear maturation of canine oocytes recovered from domestic dog ovaries. Methods Cumulus-oocyte complexes were obtained from ovaries of domestic bitches (8 months old to 7 years old), and in-vitro maturation was evaluated in TCM-199 supplemented with different concentrations (0, 20, 30 or 50%) of bovine COCs-conditioned medium (BCM). The canine COCs were cultured for 72 or 96 h at 38.5°C in 5% CO 2 , 5% O 2 and 90% N 2 . The bovine COCs-conditioned medium was obtained from culture of bovine COCs with TCM-199 supplemented with 5% FCS for 22 h at 38.5°C in 2% CO 2 , 98% air. Results The proportion of germinal vesicle breakdown (GVBD) after 72 h was significantly higher (P \ 0.05) in medium supplemented with 30% BCM (20.7%) compared with the control group (13.4%). The rates of GVBD-MII stage were significantly higher (P \ 0.05) when oocytes were matured with BCM at concentration of 30% (41.5%) compared with control (26.6%) after 72 h in-vitro culture. After 96 h in-vitro culture, the oocytes matured in medium supplemented with 30% BCM (5.5%) showed a significant increase (P \ 0.05) in the proportion of MII compared with control (0.7%). However, increasing the cultivation time from 72 to 96 h resulted in an increase in oocyte degeneration rate. Conclusions The results suggested that bovine COCsconditioned medium supplementation significantly increased nuclear maturation of canine oocytes.

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Section: Methodsmentioning

confidence: 99%

Effect of bovine cumulus–oocyte complexes‐conditioned medium on in‐vitro maturation of canine oocytes

Abdel-Ghani

Abe

Asano

et al. 2010

Reprod Medicine & Biology

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“…After being measured, only oocytes > 110 µm were selected and used for this study, because a difference in maturation rate has been found for canine oocytes with diameters < 110 µm versus > 110 µm [16,17].…”

Section: Oocyte Preparation and Culturementioning

confidence: 99%

Influence of maturation culture period on the development of canine oocytes after in vitro maturation and fertilization

et al. 2004

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-The objective of this study was to determine an optimum maturation period of canine oocytes for the development in vitro after in vitro fertilization (IVF). Canine oocytes larger than 110 micrometers in diameter, which were collected from ovaries at the follicular phase of the reproductive cycle, were cultured for each time (48, 72 and 96 h) in TCM 199 medium supplemented with 10% canine serum, fertilized, and then cultured in vitro for 8 days. Significantly more oocytes reached metaphase II (MII) in the 72-h culture group than in the 48-h culture group (25.6% vs. 41.0%). The percentages of oocytes that reached MII or beyond after maturation culture did not differ significantly between the 72-and 96-h culture groups, but the percentage of parthenogenetically activated oocytes in the 96-h culture group was significantly higher than that in the 72-h culture group. The percentages of cleaved embryos after IVF were significantly higher in the 48-and 72-h culture groups than in the 96-h culture group. In the 48-h culture group, 3.9% of fertilized oocytes developed to the 16-cell stage or beyond, but none of the cleaved embryos in the 72-and 96-h culture groups developed to the same stage. These results indicate that full nuclear maturation of oocytes collected from ovaries at the follicular phase occurs after 72 h of in vitro culture. However, an optimum maturation period (48 h) for the in vitro development of canine oocytes after IVF may be different from the period necessary to reach the maximal oocyte maturation rate, when based on the developmental stage of the cleaved embryos.developmental competence / incubation duration / meiotic maturation / oestrous stage

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“…Age (Otoi et al, 2000) that large oocytes (>120 µm diameter) had a higher frequency of maturation than oocytes (<110 µm diameter). This result also suggests the importance of selecting good quality of cumulus-oocyte complexes (COCs) by morphological appearance for IVM of oocytes.…”

Section: Introductionmentioning

confidence: 99%

“…In vitro maturation, generally has resulted in low rates of successful resumption of meiosis, with the exception being one study that found that use of oocytes from follicles larger than 2 mm yielded significantly higher rates of metaphase II development in vitro (Songsasen and Wildt, 2005), where 79.5% of oocytes recovered from follicles 2 mm size reached the second metaphase (MII), usually less than 20% of canine oocytes achieve nuclear maturation (Songsasen and Wildt, 2007). Moreover, only a single blastocyst has been produced after in vitro fertilization of in vitro matured oocytes (Otoi et al, 2000).…”

Section: Introductionmentioning

confidence: 99%

The influence of ascorbic acid on in vitro maturation of canine oocytes

Pernes¹,

Miclea²,

Zăhan³

et al. 2016

BUASVMCN-ASB

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It is known that L-ascorbic acid (vitamin C) can modulate many biochemical processes intracellularly or extracellularly as antioxidant. The aim of the present study was to examine the effects of media supplementation with ascorbic acid on canine oocyte meiotic maturation, viability and the cumulus cell expansion. Various concentrations of ascorbic acid supplemented in in vitro maturation (IVM) media were tested. Canine oocyte was exposed to different levels of ascorbic acid (0, 50, 150, 250, 500, 750µM). Cumulus expansion, meiotic maturation and degeneration of oocytes were assessed 72 h after in vitro culture. As results, on the group treated with 250µM ascorbic acid was a significant difference compared to the control group on nuclear maturation in stages metaphase I (MI) and metaphase II (MII) (26.98% vs. 6.00%). The groups treated with 50, 150, 250, 500µM had an increase in stage (GVBD), and a significant decrease of degenerate-undefined oocytes compared with the control (23.31%, 18.85%, 13.41% vs 40.80). Concentration 750µM had similar effect to that in the control group. The groups treated with 50, 150, 250, 500µM had an increase in meiosis resumption (GVBD), metaphase I (MI) and metaphase II (MII) with the best result in the group treated with 250 µM ascorbic acid.

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Canine oocyte diameter in relation to meiotic competence and sperm penetration

Cited by 78 publications

References 22 publications

Effect of bovine cumulus–oocyte complexes‐conditioned medium on in‐vitro maturation of canine oocytes

Effect of bovine cumulus–oocyte complexes‐conditioned medium on in‐vitro maturation of canine oocytes

Influence of maturation culture period on the development of canine oocytes after in vitro maturation and fertilization

The influence of ascorbic acid on in vitro maturation of canine oocytes

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