Canine pyometra: a study of the urinary proteins by SDS–PAGE and Western blot

Zaragoza, Concepción; Barrera, Rafael; Centeno, Francisco; Tapia, José A.; Mañé, M. C.

doi:10.1016/j.theriogenology.2003.07.019

Cited by 28 publications

(49 citation statements)

References 22 publications

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“…In the study, azotemic patients were similar into groups, the results suggest that Gram positive bacteria also cause kidney damage. Studies that assessed the origin of the renal lesions in dogs with pyometra by urinary protein excretion and renal histopathology, showed the cause of renal injury may be multifactorial and its location is mixed, glomerular, tubular and interstitial (ZARAGOZA et al, 2004;HEIENE et al, 2007;MADDENS et al, 2011). However, the bacterial etiology was not addressed in these studies.…”

Section: Introductionmentioning

confidence: 99%

The importance of Gram positive bacteria as the cause of canine pyometra

anna

Trautwein

Pretto‐Giordano

et al. 2017

SCA

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E. coli is the main bacteria isolated from infected uterus and bacterial endotoxin can lead to fatal endotoxic shock. Systemic inflammatory response syndrome (SIRS) precedes the endotoxic shock. Thus, early recognition of SIRS is important for patient treatment and prognostic. In Brazil, Gram positive bacteria are responsible for approximately 20% of all pyometra cases, and there is limited information about pathophysiology of shock and tissue injury. The aim of this study was to investigate the capacity of Gram positive bacteria to cause SIRS in bitches with pyometra. A prospective follow-up of 67 bitches with pyometra was performed, which were classified as SIRS + and SIRS-on admission. All bitches were surgically treated (ovariohysterectomy), uterine contents were collected in a sterile manner and the material was submitted to microbiological evaluation. Were identified in 55.2% of bitches E. coli (G1), 23.9% other Gram negative bacteria (G2) and 20.9% Gram positive bacteria (G3). The leukocyte profile, serum biochemistry and prevalence of SIRS were similar between the groups. It is concluded that Gram positive bacteria have the capacity to promote tissue damage and can lead the patient to death after SIRS and shock, as well as by E. coli and other Gram negative. Key words: Bacteria etiology. Cystic endometrial hyperplasia. SIRS. ResumoA Escherichia coli é reportada como principal agente etiológico da piometra canina e as endotoxinas bacterianas podem desencadear choque endotóxico e óbito. A síndrome da resposta inflamatória sistêmica (SIRS) antecede o choque e sua identificação pode predizer o prognóstico do paciente. No Brasil, bactérias Gram positivas são responsáveis por aproximadamente 20% dos casos de piometra, e informações a respeito da fisiopatologia do choque ou da lesão tecidual nesses casos são escassos. Assim, o objetivo foi investigar a capacidade de bactérias Gram positivas desencadear SIRS em cadelas com piometra. Foram acompanhadas de maneira prospectiva 67 cadelas com piometra, que na admissão passaram por exame clínico, exames laboratoriais e foram classificadas como SIRS+ e SIRS-. Todos os animais foram tratados cirurgicamente (ovariohisterectomia), o conteúdo uterino foi coletado de forma estéril e o material foi submetido à avaliação microbiológica. Em 55,2% das cadelas foi identificada E. coli (G1), 23,9% outras bactérias Gram negativas (G2) e 20,9% Gram positivas (G3). O perfil leucocitário, a bioquímica sérica e a prevalência de SIRS foram semelhantes entre os grupos. Conclui-se

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Section: Introductionmentioning

confidence: 99%

The importance of Gram positive bacteria as the cause of canine pyometra

anna

Trautwein

Pretto‐Giordano

et al. 2017

SCA

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“…O gel de empilhamento foi confeccionado sob concentração de 4% e o gel de separação a 10%, com espessura, largura e altura aproximadas ao tamanho da placa (Zaragoza et al 2003a(Zaragoza et al , 2003b(Zaragoza et al , 2004. A quantidade de proteína na urina aplicada no gel foi padronizada em 5µg/poço.…”

Section: Methodsunclassified

Avaliação da albuminúria e da eletroforese de proteínas urinárias de cães com hiperadrenocorticismo e a relação com a pressão arterial sistêmica

et al. 2013

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ABSTRACT.-Cavalcante C.Z., Kogika M.M., Bacic A., Santoro M.L., Miyashiro S.I., Sault J.P., Oyafuso M. Hyperadrenocorticism is one of the commonest endocrinopathies in dogs, and it is characterized by the excessive exposure of glucocorticoids excreted by adrenals. Chronic hypercortisolemia may promote several complications, including systemic hypertension and glomerulonephritis. Glomerulonephritis may initiate several variable degrees of proteinuria and leading to the development of chronic kidney disease. The loss of proteins through urine, mainly predominant albumin, is a characteristic of glomerular diseases and the determination of laboratorial variables, such as the urinary protein-to-creatinine ratio (UPC), urinary albumin-to--creatinine ratio (UAC; ELISA test) and electrophoresis of urinary proteins are recommended to elucidate the diagnosis. Therefore, the goal of this study is to evaluate the relationship between proteinuria and systemic arterial hypertension in dogs with hyperadrenocorticism and to determine through evaluation of albuminuria and molecular weight of urinary proteins, the segment of the nephron that could be damaged. Thirty dogs with hyperadrenocorticism were evaluated and subdivided into groups; 13 dogs with systemic arterial hypertension (group I) and 17 normotensive (group II). The UPC was determined, as well as UAC and the urine protein electrophoresis by polyacrylamide gel technique, containing dodecyl sodium sulphate (SDS-PAGE). The results were compared with data obtained from 30 clinically healthy dogs. No association between systemic arterial hypertension and albuminuria was detected in dogs with hyperadrenocorticism as well as no alterations of proteins patterns or molecular weights bands of low (<60 kDa) or high molecular weight (> 60 kDa) was found. However, dogs with hyperadrenocorticism may develop glomerular and tubular injuries that were characterized by the presence of albuminuria and proteins of low and high molecular weights, independently of systemic arterial hypertension. In conclusion, the quantitative (UPC and UAC) and qualitative (SDS-PAGE) evaluation of urinary proteins could add information to indicate the possible segments of the nephrons that caused the loss of those proteins.

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“…O gel de empilhamento foi confeccionado em uma concentração de 4% e o gel de separação a 10% com espessura, largura e altura semelhante ao tamanho da placa (LAEMLI, 1970;ZARAGOZA et al, 2003ZARAGOZA et al, , 2004 Para avaliação dos pesos moleculares e da densidade óptica das proteínas urinárias foi utilizado um programa de computador específico supracitado. As regiões individuais do gel que corresponde a aplicação de cada amostra foi chamada de faixa (lane), sendo que cada faixa apresenta diferentes bandas de proteínas com pesos moleculares distintos.…”

Section: Eletroforese De Proteínas Urináriasunclassified

“…Na região A estão localizadas proteínas com peso molecular maior ou igual a 60KDa, enquanto que a região B é destinada para proteínas com peso molecular inferior a 60KDa, para classificar a proteinúria der acordo com o peso molecular (OSBORNE et al, 1995;ZARAGOZA et al,2003ZARAGOZA et al, , 2004CAVALCANTE, 2007).…”

Section: Eletroforese De Proteínas Urináriasunclassified

“…A análise qualitativa das proteínas urinárias pela eletroforese com o gel de poliacrilamida pode auxilar na detecção precoce de lesão renal, pois a mudança do perfil de bandas de proteínas de acordo com o peso molecular pode indicar o local de origem da alteração renal que possa estar acometido (ZARAGOZA et al, 2003(ZARAGOZA et al, , 2004LEES et al, 2005;GRAUER, 2011).…”

Section: Eletroforese De Proteínas Urináriasunclassified

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Avaliação sequencial da proteinúria, albuminúria e eletroforese de proteínas urinárias durante a evolução do diabetes mellitus em cães

Martorelli¹

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Canine pyometra: a study of the urinary proteins by SDS–PAGE and Western blot

Cited by 28 publications

References 22 publications

The importance of Gram positive bacteria as the cause of canine pyometra

The importance of Gram positive bacteria as the cause of canine pyometra

Avaliação da albuminúria e da eletroforese de proteínas urinárias de cães com hiperadrenocorticismo e a relação com a pressão arterial sistêmica

Avaliação sequencial da proteinúria, albuminúria e eletroforese de proteínas urinárias durante a evolução do diabetes mellitus em cães

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