Cannabidiol and Nano-Selenium Increase Microvascularization and Reduce Degenerative Changes in Superficial Breast Muscle in C. perfringens-Infected Chickens

Abstract

Here, we demonstrated the potential of Cannabis-derived cannabidiol (CBD) and nanosized selenium (nano-Se) for the modulation of microvascularization and muscle fiber lesions in superficial breast muscle in C. perfringens-challenged chickens. The administration of CBD resulted in a decreased number of atrophic fibers (3.13 vs. 1.13/1.5 mm2) compared with the control, whereas nano-Se or both substances resulted in a decreased split fiber number (4.13 vs. 1.55/1.5 mm2) and in a lower number of necrotic myofibers (2.38 vs. 0.69/1.5 mm2) in breast muscle than the positive control. There was a significantly higher number of capillary vessels in chickens in the CBD+Nano-Se group than in the control and positive control groups (1.31 vs. 0.97 and 0.98, respectively). Feeding birds experimental diets lowered the activity of DNA damage repair enzymes, including 3,N4-ethenodeoxycytosine (by 39.6%), 1,N6-ethenodeoxyadenosine (by 37.5%), 8-oxo-guanine (by 36.2%), formamidopyrimidine (fapy)-DNA glycosylase (by 56.2%) and human alkyl adenine DNA glycosylase (by 40.2%) in the ileal mucosa, but it did not compromise the blood mitochondrial oxygen consumption rate (−2.67 OD/min on average). These findings indicate a potential link between gut mucosa condition and histopathological changes in superficial pectoral muscle under induced inflammation and show the ameliorative effect of CBD and nano-Se in this cross-talk due to their protection from mucosal DNA damage.

Cannabinoids: Potential for Modulation and Enhancement When Combined with Vitamin B12 in Case of Neurodegenerative Disorders

“…Konieczka et al, 2022 [109] In studies using selenium, the addition of CBD and Nano-Se resulted in a significantly higher number of capillaries in the muscle tissues compared to groups treated with selenium alone or the control groups (p < 0.05). Furthermore, chickens infected with C. perfringens and treated with both CBD and Nano-Se showed fewer necrotic muscle fibers, indicating a protective effect from the combination of these bioactive substances.…”

“…Vitamin B12, or cobalamin, which is essential for DNA synthesis, red blood cell formation, neurological function, and the metabolism of fatty acids and amino acids, might also interact beneficially with cannabinoids. This potential synergy is intriguing, given the positive effects observed with omega-3 fatty acids in managing conditions like ALS [ 2 ], and the enhanced efficacy of CBD when combined with selenium in reducing cancer tissues [ 109 ]. Furthermore, the role of microglia in maintaining CNS homeostasis and their response to neurodegenerative stressors highlights the importance of exploring combinations of cannabinoids with vitamin B12.…”

“…Expanding on this narrative, studies exploring the inclusion of CBD as an additive compound in therapy have demonstrated an increased therapeutic effect when combined with specific compounds; for instance, in the combination with selenium, the CBD + Nano-Se group exhibited a significantly higher number of capillaries in chicken muscles compared to the control group or selenium alone ( p < 0.05). This combination also resulted in a reduced number of necrotic muscle fibers in chickens infected with C. perfringens [ 109 ]. This enhanced effect of including CBD is crucial not only from a cancer perspective but also in the context of neurodegenerative diseases, as corroborated by studies on selenium in HD treatment.…”

“…The molecular mechanism revealed that Nano-Se attenuated oxidative stress, inhibited huntingtin protein aggregation, and downregulated the expression of histone deacetylase family members at mRNA levels [ 124 ]. Additionally, research on the sigma-1 receptor as a protective factor for diabetes-related cognitive dysfunction, involving the regulation of astrocyte endoplasmic reticulum–mitochondria contact and endoplasmic reticulum stress, is worth mentioning [ 109 ]. There is also an observation regarding the possible susceptibility in elderly patients with HD to the development of ALS features with an atypical distribution of TDP-43 that resembles aggregated mutant huntingtin.…”

“…These combined strategies suggest a robust approach to potentially improving outcomes in neurodegenerative disease treatments by addressing both delivery challenges and therapeutic effectiveness. Combination of Nano-Selenium (Nano-Se) with CBD as an Example of CBD Enhancement Impact on Other Substances Konieczka et al, 2022 [ 109 ] In studies using selenium, the addition of CBD and Nano-Se resulted in a significantly higher number of capillaries in the muscle tissues compared to groups treated with selenium alone or the control groups ( p < 0.05). Furthermore, chickens infected with C. perfringens and treated with both CBD and Nano-Se showed fewer necrotic muscle fibers, indicating a protective effect from the combination of these bioactive substances.…”