Cannabidiol reduces neuroinflammation and promotes neuroplasticity and functional recovery after brain ischemia

Mori, Marco; Meyer, Erika; Soares, Lígia Mendes; Milani, Humberto; Guimarães, Francisco Silveira; Oliveira, Rúbia Maria Weffort de

doi:10.1016/j.pnpbp.2016.11.005

Cited by 122 publications

(122 citation statements)

References 85 publications

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“…The results showed that ischemic rats had significant higher number of TUNEL-positive cells and lower number of alive cells compared to the control. To explain, the same results were obtained with 20 min induction of ischemia by BCCAO in the works performed by Sharifi et al [20], Movassaghi et al [21] in rats and Mori et al [22] in mice. The results of TUNEL assay and Nissl staining in the ischemic rats were significantly counteracted by combined application of AM251 and JWH-015, as compared with each of the ischemia, AM251, and JWH-015 groups.…”

Section: Discussionsupporting

confidence: 70%

Combined Neuroprotective Action of JWH-015 and AM251 in the CA1 Hippocampal Area of Transient Global Cerebral Ischemia

Shiasi¹,

Mortezaee²,

Abolhassani³

et al. 2017

Preprint

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Transient global cerebral ischemia (TGCI) induces by bilateral common carotid artery occlusion (BCCAO), and it mediates neuronal cell death of the CA1 hippocampal area. AM251 is a cannabinoid receptors type 1 (CB1) blocker that has been known to be protective against transient focal cerebral ischemia. JWH-015 is a selective agonist of CB2 and activator of CB1 that is involved in promotion of neuronal recovery and survival. The role of combined application of JWH-015 with AM251 in the rat model of GCI has been surveyed in this study. Male Wistar rats underwent 20 min of ischemia followed by reperfusion. Then, 1 mg/kg JWH-015 and 2 mg/kg AM251 were administered through caudal vein. The groups were control, sham, ischemia, vehicle, AM251, JWH-015 and AM251 + JWH-015. Animals were sacrificed at 14 days after reperfusion. The AM251 + JWH-015 group showed a significant increase in the protein expressions of AKT1, Bad 14-3-3, Bcl-2 and Bcl-XL, but it showed a considerable decrease in the protein expressions of Bad and JNK1/2 (p ≤ 0.05 vs. AM251, and JWH-015 groups). The AM251 + JWH-015 group had a significant higher number of alive cells and lower number of TUNEL-positive cells in the CA1 hippocampal area, and it also had a considerable improvement of spatial memory (p ≤ 0.05 vs. AM251, and JWH-015 groups). The results of this study showed that combined application of AM251 and JWH-015 could be neuroprotective against detrimental effects of ischemia probably via suppression of neuronal apoptosis and maintenance of their survival.

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Section: Discussionsupporting

confidence: 70%

Combined Neuroprotective Action of JWH-015 and AM251 in the CA1 Hippocampal Area of Transient Global Cerebral Ischemia

Shiasi¹,

Mortezaee²,

Abolhassani³

et al. 2017

Preprint

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“…Furthermore, CBD was proposed as pharmacotherapy for the treatment of several diseases including cancer, epilepsy, cerebral ischemia, multiple sclerosis, anxiety, and trauma-related and psychiatric disorders. [22][23][24][25][26][27][28] Measuring CBD in urine would be a useful tool to monitor compliance with prescribed therapy.…”

mentioning

confidence: 99%

Optimization of recombinant β‐glucuronidase hydrolysis and quantification of eight urinary cannabinoids and metabolites by liquid chromatography tandem mass spectrometry

Sempio

Scheidweiler

Barnes

et al. 2017

Drug Testing and Analysis

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Prolonged urinary cannabinoid excretion in chronic frequent cannabis users confounds identification of recent cannabis intake that may be important in treatment, workplace, clinical, and forensic testing programs. In addition, differentiation of synthetic Δ9-tetrahydrocannabinol (THC) intake from cannabis plant products might be an important interpretive issue. THC, 11-hydroxy-THC (11-OH-THC) and 11-nor-9-carboxy-THC (THCCOOH) urine concentrations were evaluated during previous controlled cannabis administration studies following tandem alkaline/E. coli β-glucuronidase hydrolysis. We optimized recombinant β-glucuronidase enzymatic urinary hydrolysis before simultaneous liquid chromatography tandem mass spectrometry (LC-MS/MS) quantification of THC, 11-OH-THC, THCCOOH, cannabidiol (CBD), cannabinol (CBN), cannabigerol (CBG), tetrahydrocannabivarin (THCV) and 11-nor-9-carboxy-THCV (THCVCOOH) in urine. Enzyme amount, incubation time and temperature, buffer molarity and pH were optimized using pooled urine samples collected during a National Institute on Drug Abuse, Institutional Review Board-approved clinical study. Optimized cannabinoid hydrolysis with recombinant β-glucuronidase was achieved with 2000 IU enzyme, 2 M pH 6.8 sodium phosphate buffer, and 0.2 mL urine at 37°C for 16 h. The LC-MS/MS quantification method for hydrolyzed urinary cannabinoids was validated per the Scientific Working Group on Toxicology guidelines. Linear ranges were 1-250 μg/L for THC and CBG, 2-250 μg/L for 11-OH-THC, CBD, CBN, THCV and THCVCOOH, and 1-500 μg/L for THCCOOH. Inter-batch analytical bias was 92.4-112.4%, imprecision 4.4-9.3% CV (n = 25), extraction efficiency 44.3-97.1% and matrix effect -29.6 to 1.8% (n = 10). The method was utilized to analyze urine specimens collected during our controlled smoked, vaporized, and edible cannabis administration study to improve interpretation of urine cannabinoid test results.

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“…Por meio de comunicação oral, foram apresentadas a utilização de psicodélicos, ou seja, o uso de substâncias que de alguma forma alteram as manifestações normais da mente, (OSMOND, 1957), por um pesquisador convidado e duas acadêmicas: i) "Neurotransmissão serotoninérgica e canabinóides", um palestrante convidado abordou os seguintes tópicos: i) "Evolução do Sistema Nervoso Central" (e. g. OLKOWICZ et al, 2016); ii) "Sistemas de Neurotransmissão" (e. g. KANDEL et al, 2014); iii) "Vias Dopaminérgicas" (e. g. RANG et al, 2007); iv) "Sistema serotoninérgico" (e. g. RANG et al, 2007); v) "Drogas que alteram o sistema de neurotransmissão"; (e. g. RANG et al, 2007); vi) "Sistema serotoninérgico e a ação de drogas alucinógenas" (e. g. NICHOLS, 2004); vii) "Aplicabilidade clínica e experimentais de drogas que agem no sistema serotoninérgico" (LAU; SCHLOSS, 2008) e viii) "Ca-nabidiol em modelos animais de isquemia cerebral" (MORI et al, 2017). Os quatro tópicos iniciais foram introdutórios e serviram como base para o público acompanhar os tópicos subsequentes, que eram mais específicos.…”

Section: Apresentação Da Mesa Redonda Sobre O Segundo Cérebrounclassified

“…As respostas a esses questionamentos foram respectivamente: i)" há pesquisas sendo realizadas e medicamentos no mercado sobre o uso do canabidiol no tratamento para epilepsia" (e. g. DE CAR-VALHO et al, 2017), ii) "No Brasil há uma série de questões burocráticas e éticas que devem ser cumpridas" (e .g. GRIPP, 2017), iii) "as pesquisas até então não apresentaram efeitos colaterais" (MORI et al, 2017); Em relação às perguntas direcionadas a apresentação sobre a Ayahuasca foi: i) "como se pode evitar a síndrome serotoninérgica? ", ii) "quais os efeitos da bebida a longo prazo?…”

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Semana do cérebro: divulgando a neurociência e integrando ensino, pesquisa e extensão

Macacare¹,

Rocha

Cruz

et al. 2018

Exp. Ext.

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A Neurociência, ciência responsável pelo estudo do sistema nervoso, têm crescido exponencialmente nas últimas décadas. Visto que muitos assuntos relacionados ao tema fazem parte do cotidiano popular, a divulgação científica das neurociências se torna relevante. Desta forma, o Grupo de Estudos em Neurociências da Universidade Estadual do Norte do Paraná (GEN / UENP) aderindo evento mundial de divulgação neurocientífica “Brain Awareness Week” (DANA Foundation), organizou a III Semana do Cérebro da UENP. O objetivo do presente trabalho é relatar sobre a ação presencial da III Semana do Cérebro da UENP, realizada em 2017, mostrando como um evento extensionista pode contribuir na formação dos alunos envolvidos e como ele integra ensino, pesquisa e extensão, tripé que é premissa da Universidade. Em dois dias de evento foram trabalhados diversos temas das neurociências que envolveram apresentação de filmes, palestras e discussões em forma de debate. Mais de 300 pessoas foram alcançadas com essa ação e pela quantidade de questionamentos levantados pelos participantes, podemos notar o interesse do público acerca da temática. A participação dos alunos do GEN como palestrantes aponta que eventos extensionistas podem ser utilizados como instrumento pedagógico importante na qualificação profissional dos acadêmicos.

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Cannabidiol reduces neuroinflammation and promotes neuroplasticity and functional recovery after brain ischemia

Cited by 122 publications

References 85 publications

Combined Neuroprotective Action of JWH-015 and AM251 in the CA1 Hippocampal Area of Transient Global Cerebral Ischemia

Combined Neuroprotective Action of JWH-015 and AM251 in the CA1 Hippocampal Area of Transient Global Cerebral Ischemia

Optimization of recombinant β‐glucuronidase hydrolysis and quantification of eight urinary cannabinoids and metabolites by liquid chromatography tandem mass spectrometry

Semana do cérebro: divulgando a neurociência e integrando ensino, pesquisa e extensão

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