Cannabinoid Biosynthesis Using Noncanonical Cannabinoid Synthases

Go, Maybelle Kho; Zhu, Tingting; Lim, Kevin; Hartono, Yossa Dwi; Xue, Bin; Fan, Hao; Yew, Wen Shan

doi:10.3390/ijms24021259

Cited by 8 publications

(4 citation statements)

References 21 publications

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“…Fiber production from hemp has been conducted over many centuries, for end uses ranging from textiles and papers (hemp paper was used in the first copies of the Bible) to ropes and sails [ 11 ]. Cannabis has been widely cultivated due to its industrial [ 12 ], ornamental [ 13 ], nutritional [ 14 ], medicinal and recreational [ 15 ] potential. From regulatory and application perspectives, cannabis plants are categorized based on the level of Δ9-tetrahydrocannabinol (THC), one of the most important phytocannabinoids [ 16 ].…”

Section: Introductionmentioning

confidence: 99%

First Report of Hemp Fusarium Wilt Caused by Fusarium oxysporum in Croatia

Duvnjak,

Vrandecic,

Sudaric

et al. 2023

Plants

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Wilted hemp (Cannabis sativa L.) plants were observed in August 2019 in commercial fields around Osijek, Croatia. Plants and roots with disease symptoms were collected. The single-spored isolates produced septate cottony white to light pink aerial mycelium and purple undersurface on potato dextrose agar (PDA). Smooth and hyaline hyphae were branched and septate. Macroconidia were fusiform to sickle-shaped with foot-shaped basal cells, elongated apical cells and three to five septa. Sequencing of the internal transcribed spacer and the partial elongation factor 1-α gene identified the species as Fusarium oxysporum. Artificial infection fulfills Koch’s postulates, producing plants which show stunted growth and wilt symptoms similar to those observed in the commercial fields. Control seedlings remained symptomless and healthy. To the best of our knowledge, this is the first report of hemp Fusarium wilt causing F. oxysporum in Croatia. Considering that F. oxysporum has been reported in main field crops in Croatia, the presence of this pathogen could cause economically significant hemp production decreases, especially in humid and cold springs and susceptible varieties.

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Section: Introductionmentioning

confidence: 99%

First Report of Hemp Fusarium Wilt Caused by Fusarium oxysporum in Croatia

Duvnjak,

Vrandecic,

Sudaric

et al. 2023

Plants

View full text Add to dashboard Cite

show abstract

“…Fiber production from hemp has been conducted over many centuries, for end uses ranging from textiles and papers (hemp paper was used in the first copies of the Bible) to ropes and sails [11]. Cannabis has been widely cultivated due to its industrial [12], ornamental [13], nutritional [14], medicinal and recreational [15] potential. From regulatory and application perspectives, cannabis plants are categorized based on the level of ∆9-tetrahydrocannabinol (THC), one of the most important phytocannabinoids [16].…”

Section: Introductionmentioning

confidence: 99%

First Report of Hemp Fusarium wilt Caused by <em>Fusarium oxysporum</em> in Croatia

Duvnjak¹,

Vrandečić²,

Sudarić³

et al. 2023

Preprint

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Wilted hemp (Cannabis sativa L.) plants were observed in August 2019 in commercial fields around Osijek, Croatia. Plants and roots with disease symptoms were collected. The single-spored isolates produced septate cottony white to light pink aerial mycelium and purple undersurface on potato dextrose agar (PDA). Smooth and hyaline hyphae were branched and septate. Macroconidia were fusiform to sickle-shaped with foot-shaped basal cell, elongated apical cell and three to five septa. Sequencing of the internal transcribed spacer and the partial elongation factor 1-α gene identified species as Fusarium oxysporum. Artificial infection fulfills Koch’s postulates, producing plants which showed stunted growth and wilt symptoms similar to those observed in the commercial fields. Control seedlings remained symptomless and healthy. To the best of our knowledge, this is the first report of hemp Fusarium wilt causing F. oxysporum in Croatia. Considering that F. oxysporum has been reported in main field crops in Croatia, the presence of this pathogen could cause economically significant hemp production decreases, especially in humid and cold springs and susceptible varieties.

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“…One of the main non-psychotropic phytocannabinoids is CBG, whose biosynthesis is characteristic especially for plants of the species Cannabis sativa L. (Beleggia et al, 2023). Its synthesis primarily depends on plant genotype; however, due to the complexity of the process of extraction and purification of the compound, CBG used in medicine often comes from in vitro enzymatic synthesis (Qamar et al, 2021;Go et al, 2023). Its biological action is based on the activation of cannabinoid receptors (CB1/2) (Makwana et al, 2015;Navarro et al, 2018;Di Meo et al, 2022), resulting in the modulation of expression of proinflammatory proteins, such as TNF-α, IL-1β, IL-6, IL-10, interferon-γ, and prostaglandin E2 (Borrelli et al, 2013;Petrosino et al, 2018;Nachnani et al, 2021).…”

Section: Introductionmentioning

confidence: 99%

The Anti-Inflammatory Action of Cannabigerol Accompanied by the Antioxidant Effect of 3-O-ethyl Ascorbic Acid in UVA-Irradiated Human Keratinocytes

Gęgotek,

Jarocka-Karpowicz,

Atalay Ekiner

et al. 2023

J Pharmacol Exp Ther

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Excessive daily exposure of human skin to natural UVA radiation leads to impaired redox homeostasis in epidermal keratinocytes, resulting in changes in their proteome. Commonly used antioxidants usually exhibit protection in a narrowed range, which makes it necessary to combine their effects. Therefore, the aim of this study was to analyze the protective effect of cannabigerol (CBG) and 3-O-ethyl ascorbic acid (EAA), used separately and together, on the proteomic profile of UVA irradiated keratinocytes. Proteomic analysis with the use of the Q Exactive HF mass spectrometer, combined with biostatistic tests, performed on UVAirradiated keratinocytes indicated enhanced and lowered expression of 186 and 160 proteins, respectively. CBG treatment after UVA irradiation reduced these numbers to 110 upregulated and 49 downregulated proteins, while EAA eliminated all these changes. CBG completely eliminated the UV-induced effect on the expression of pro-inflammatory proteins and significantly increased the level of proteins responsible for cellular locomotion. On the other hand, CBG reduced the level of UVA-induced 4-HNE-protein adducts 5fold, whereas EAA had no effect on this modification. At the same time, CBG and EAA did not modify the expression/structure of proteins in relation to the non-irradiated control keratinocytes in the case of an unaccompanied use, or slightly modified the protein profile when used in a mixture. The combined protective effects of CBG on protein structure and EAA on protein expression profile allowed to obtain a wider protection of cells against UVA radiation, compared to when the compounds were used alone.

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Cannabinoid Biosynthesis Using Noncanonical Cannabinoid Synthases

Cited by 8 publications

References 21 publications

First Report of Hemp Fusarium Wilt Caused by Fusarium oxysporum in Croatia

First Report of Hemp Fusarium Wilt Caused by Fusarium oxysporum in Croatia

First Report of Hemp Fusarium wilt Caused by <em>Fusarium oxysporum</em> in Croatia

The Anti-Inflammatory Action of Cannabigerol Accompanied by the Antioxidant Effect of 3-O-ethyl Ascorbic Acid in UVA-Irradiated Human Keratinocytes

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