Capacity for eicosapentaenoic acid and arachidonic acid biosynthesis in silver barb (Barbonymus gonionotus): Functional characterisation of a Δ6/Δ8/Δ5 Fads2 desaturase and Elovl5 elongase

Janaranjani, M.; Mah, Min-Qian; Kuah, Meng-Kiat; Fadhilah, Nor; Hing, Sher-Ryn; Han, Wan-Yin; Shu‐Chien, Alexander Chong

doi:10.1016/j.aquaculture.2018.08.019

Cited by 25 publications

(27 citation statements)

References 70 publications

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“…In the present study, the expression of Δ6 fad and elovl5 transcripts in the liver and muscle of silver barbs was significantly higher in VO diet‐fed groups, in comparison to that of the FO (control diet) diet‐fed group. The present observations are in accordance with the earlier observation in this species, in which the authors reported that the expression of elongase and desaturase enzymes in liver and muscle tissues was increased with the inclusion of VO in the diets. The expression of Δ6 fad and elovl5 depends on the functional capacity and substrate specificity.…”

Section: Discussionsupporting

confidence: 94%

“…The dietary ALA:LA ratio did influence the growth performance of juvenile yellow catfish, tench, large yellow croakers, and groupers. 18, In the present experiment, the average percentage body weight gain of the fingerlings was 167%, which is similar to the earlier observations in this species, at this body weight range . The dietary ALA:LA ratio did not influence the feed intake in silver barbs, which is similar to the earlier observation in Murray cod .…”

Section: Discussionsupporting

confidence: 91%

“…The expression of Δ6 fad and elovl5 depends on the functional capacity and substrate specificity. In silver barb, Δ6 fad induced conversion rate of n3‐PUFA to LC‐PUFA is more than that of n6‐PUFA and Elovl5 is having more preference on C18 PUFA than C20 PUFAs for elongation reaction . However, in bluefin tuna andNorthern pikeElovl5 did not show any preference for C18 or C20 PUFAs for elongation reaction.…”

Section: Discussionmentioning

confidence: 88%

“…Earlier, we observed that bioconversion of C18 PUFA to LC‐PUFA in silver barb is possible when dietary fish oil is replaced with linseed oil . In silver barb, the genes encoding desaturase ( Δ6 fad ) and elongase (Elovl5) in LC‐PUFA biosynthetic pathways have been cloned and functionally characterized …”

Section: Introductionmentioning

confidence: 99%

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Effects of dietary α‐linolenic acid/linoleic acid ratio on growth performance, tissue fatty acid profile, serum metabolites and Δ6 fad and elovl5 gene expression in silver barb (Puntius gonionotus)

et al. 2019

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Effects of dietary -linolenic acid/linoleic acid ratio on growth performance, tissue fatty acid profile, serum metabolites and 6 fad and elovl5 gene expression in silver barb (Puntius gonionotus) Abstract BACKGROUND: Silver barb (Puntius gonionotus) is a medium-sized carp that is promising for freshwater aquaculture in Asia. This study's aim was to investigate the ideal dietary -linolenic acid (ALA): linoleic acid (LA) ratio for maximizing long-chain polyunsaturated fatty acid (LC-PUFA) synthesis and their deposition in the muscle of silver barb, as that of fish oil based control diet.RESULT: Fish (with an initial body weight of 11.07 ± 0.12 g) were fed for 60 days with five experimental iso-proteinous, iso-lipidic, and iso-caloric diets, supplemented with linseed oil and peanut oil at varying levels to obtain ALA:LA ratios of 0.35, 0.51, 0.91, 2.04, 2.66. A control diet was prepared by supplementing fish oil. The dietary ALA:LA ratio did not influence the growth performance of fish. With increased dietary ALA:LA ratios, LA content decreased and ALA content increased in the muscle and liver of silver barb. The n-3 LC-PUFA level in muscle and liver was not influenced by feeding different ratios of ALA:LA, whereas n-6 LC-PUFA was decreased in the muscle and increased in the liver with increased dietary ALA:LA ratios. Increasing dietary ALA:LA ratio increased the 6fad and elovl5mRNA expression in the liver, muscle, brain, and intestinal tissues of silver barbs. CONCLUSION: Silver barb possess the ability to elongate and desaturate ALA and LA to their end products EPA and DHA. The highest level expression of 6 fad and elovl5 mRNA at the dietary ALA:LA ratio of 2.66 suggests greater affinity of these enzymes towards ALA than LA in silver barb.

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Section: Discussionsupporting

confidence: 94%

Section: Discussionsupporting

confidence: 91%

Section: Discussionmentioning

confidence: 88%

Section: Introductionmentioning

confidence: 99%

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Effects of dietary α‐linolenic acid/linoleic acid ratio on growth performance, tissue fatty acid profile, serum metabolites and Δ6 fad and elovl5 gene expression in silver barb (Puntius gonionotus)

et al. 2019

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“…The notable presence of B. boddarti fads2 transcript in brain tissue is similar to findings from many marine and freshwater species (Monroig et al, 2013, Ren et al, 2013, Tanomman et al, 2013, Wang et al, 2014, Kuah et al, 2016, Janaranjani et al, 2018, Xie et al, 2018). Given the importance of DHA in neuronal-rich tissues such as brain and eye, having the endogenous capacity for LC-PUFA biosynthesis is advantageous when supply from dietary intake is insufficient.…”

Section: Discussionsupporting

confidence: 80%

Functional characterization of fatty acyl desaturase Fads2 and Elovl5 elongase in the Boddart’s goggle-eyed goby,Boleophthalmus boddarti(Gobiidae) suggest an incapacity for long-chain polyunsaturated fatty acid biosynthesis

Soo

Chong

Sean³

et al. 2019

Preprint

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AbstractLong-chain polyunsaturated fatty acid biosynthesis, a process to convert C18 polyunsaturated fatty acids to eicosapentaenoic acid (EPA), docosahexaenoic acid (DHA) or arachidonic acid (ARA) requires the concerted activities of two enzymes, the fatty acyl desaturase (Fads) and elongase (Elovl). This study highlights the cloning, functional characterisation and tissue expression pattern of a Fads and Elovl from the Boddart’s goggle-eyed goby (Boleophthalmus boddarti), a mudskipper species widely distributed in the Indo-Pacific region. Phylogenetic analysis revealed that the cloned Fads and Elovl are clustered with other teleost Fads2 and Elovl5 orthologs, respectively. Interrogation of the genome of several mudskipper species, namely B. pectinirostris, Periophthalmus schlosseri and P. magnuspinnatus revealed a single Fads2 for each respective species while two elongases, Elovl5 and Elovl4 were detected. Using a heterologous yeast assay, the B. boddarti Fads2 was shown to possess low desaturation activity on C18 PUFA. In addition, there was no desaturation of C20 and C22 substrates. In comparison, the Elovl5 showed a wide range of substrate specificity, with capacity to elongate C18, C20 and C22 PUFA substrates. We identified an amino acid residue in the B. boddarti Elovl5 that affect the capacity to bind C22 PUFA substrate. Both genes are highly expressed in brain tissue. Among all tissues, DHA is highly concentrated in neuron-rich tissues while EPA is highly deposited in gills. Taken together, the results showed that due to disability of desaturation steps, B. boddarti is unable to biosynthesis LC-PUFA, relying on dietary intake to acquire these nutrients.

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Essential Fatty Acids—‘Fueling Versus Controlling’

Steinberg

2022

Aquatic Animal Nutrition

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Capacity for eicosapentaenoic acid and arachidonic acid biosynthesis in silver barb (Barbonymus gonionotus): Functional characterisation of a Δ6/Δ8/Δ5 Fads2 desaturase and Elovl5 elongase

Cited by 25 publications

References 70 publications

Effects of dietary α‐linolenic acid/linoleic acid ratio on growth performance, tissue fatty acid profile, serum metabolites and Δ6 fad and elovl5 gene expression in silver barb (Puntius gonionotus)

Effects of dietary α‐linolenic acid/linoleic acid ratio on growth performance, tissue fatty acid profile, serum metabolites and Δ6 fad and elovl5 gene expression in silver barb (Puntius gonionotus)

Functional characterization of fatty acyl desaturase Fads2 and Elovl5 elongase in the Boddart’s goggle-eyed goby,Boleophthalmus boddarti(Gobiidae) suggest an incapacity for long-chain polyunsaturated fatty acid biosynthesis

Essential Fatty Acids—‘Fueling Versus Controlling’

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