Capillary and microchip electrophoresis method development for amino acid monitoring during biopharmaceutical cultivation

Abstract

The increased use of biopharmaceuticals calls for improved means of bioprocess monitoring. In this work, capillary electrophoresis (CE) and microchip electrophoresis (MCE) methods were developed and applied for the analysis of amino acids (AAs) in cell culture supernatant. In samples from different days of a Chinese hamster ovary cell cultivation process, all 19 proteinogenic AAs containing primary amine groups could be detected using CE, and 17 out of 19 AAs using MCE. The relative concentration changes in different samples agreed well with those measured by high-performance liquid chromatography (HPLC). Compared to the more commonly employed HPLC analysis, the CE and MCE methods resulted in faster analysis, while significantly lowering both the sample and reagent consumption, and the cost per analysis.

Method development for mono‐ and disaccharides monitoring in cell culture medium by capillary and microchip electrophoresis

“…Using micellar electrokinetic chromatography could enhance separation due to the extra separation mechanism introduced by the micelles, therefore, SDS was included as a factor in the design. In addition, compatibility with other methods developed for the integrated platform such as amino acid analysis [46] would improve. The boric acid concentration, the SDS concentration, and the pH were varied in the ranges 80–160 mM, 0–100 mM, and 8.5–9.5, respectively.…”

A Novel Overlapping ME Peaks Decomposition Algorithm Based on Iterative Derivative Sharpening

“…The detection results of exosomes [1] and heavy metals [2] samples show the wide application of ME. However, the overlapping peaks problem [3], [4], [5], [6] is a serious challenge for ME signal analysis. Thus, to accurately measure component peaks in ME signals, further research is needed on the analysis of low-resolution overlapping peaks.…”

Overview of chromatographic and electrophoretic methods for the determination of branched‐chain amino acids

“…In view of the miniature scale of microchip electrophoresis (MCE), it is suitable for high throughput screening of biomarkers in metabolomics. It improves the selectivity and sensitivity of the MS for the detection and separation of metabolites by the combination of MCE with highresolution MS (HRMS) [202]. In mouse serum, Sah et al [203] targeted the metabolites associated with high-grade S5 for an overview of the CE and MCE applications.…”