Capillary and packed column SFC/MS

Abstract

SummaryA frit restrictor interface for capillary column supercritical fluid chromatography/mass spectrometry (SFC/MS) has been constructed and used for the analysis of high boiling point alkanes. Packed column SFC/MS is described using both a moving belt liquid chromatographic/mass spectrometric interface and a thermospray source in the filament-on mode.

Use and practice of achiral and chiral supercritical fluid chromatography in pharmaceutical analysis and purification

“…It was already proven that SFC–MS could compete with LC–MS for the purpose of pharmaceuticals analysis . Atmospheric pressure chemical ionization (APCI) and ESI sources are both commonly used. The use of atmospheric pressure photo‐ionization was also reported .…”

Hyphenated Methods for Identification After Chromatographic Separation

“…The basic problems of interfacing supercritical fluid chromatography to mass spectrometry are intermediate between those of gas and liquid chromatography [70][71][72][73][74]. Nearly all current research in SFC utilizes either open tubular columns of very small internal diameter, 25-100 micrometers, or slurry packed capillary or small bore columns prepared for use in liquid chromatography.…”

“…For small bore packed column SFC/MS direct fluid introduction into a modified chemical ionization source, a particle beam interface, a modified filament on thermospray interface, and the moving belt interface have been used successfully [71][72][73][81][82][83]. The moving belt interface used in liquid chromatography requires only minor modification to the spray deposition device to avoid freezing of the nozzle for SFC/MS and can be used with mixed mobile phases [82].…”

Investigation of antioxidants and UV stabilizers from plastics. Part I: Comparison of HPLC and SFC; preliminary SFC/MS study

“…Although different practical problems still remain to be solved, the coupling is rather easy to construct and the subject is now well documented [20][21][22].…”