Capillary electrochromatography using monoamine- and triamine-bonded silica nanoparticles as pseudostationary phases

Takeda, Yuto; Hayashi, Yuka; Utamura, Naonori; Takamoto, Chise; Kinoshita, Mitsuhiro; Yamamoto, Sachio; Hayakawa, Tetsuo; Suzuki, Shigeo

doi:10.1016/j.chroma.2015.11.085

Cited by 16 publications

(7 citation statements)

References 28 publications

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“…CEC separations of peptides and proteins have been performed in different separation modes, with reverse‐phase (RP) , affinity , mixed‐mode , and cationic or anionic ion‐exchange stationary phases, in classical FS capillary columns as well as in microfluidic chips . Attractive alternatives to the classical CEC columns packed with particulate materials are the monolithic stationary phases .…”

Section: Separations By the Particular Ce And Cec Methodsmentioning

confidence: 99%

Recent developments in capillary and microchip electroseparations of peptides (2015–mid 2017)

Kašička

2017

Electrophoresis

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The review brings a comprehensive overview of recent developments and applications of high performance capillary and microchip electroseparation methods (zone electrophoresis, isotachophoresis, isoelectric focusing, affinity electrophoresis, electrokinetic chromatography, and electrochromatography) to analysis, microscale isolation, purification, and physicochemical and biochemical characterization of peptides in the years 2015, 2016, and ca. up to the middle of 2017. Advances in the investigation of electromigration properties of peptides and in the methodology of their analysis (sample preseparation, preconcentration and derivatization, adsorption suppression and EOF control, and detection) are described. New developments in particular CE and CEC methods are presented and several types of their applications to peptide analysis are reported: qualitative and quantitative analysis, determination in complex (bio)matrices, monitoring of chemical and enzymatical reactions and physical changes, amino acid, sequence and chiral analysis, and peptide mapping of proteins. Some micropreparative peptide separations are shown and capabilities of CE and CEC methods to provide important physicochemical characteristics of peptides are demonstrated.

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Section: Separations By the Particular Ce And Cec Methodsmentioning

confidence: 99%

Recent developments in capillary and microchip electroseparations of peptides (2015–mid 2017)

Kašička

2017

Electrophoresis

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“…Currently, Takeda et al. prepared amine‐bonded silica NPs and used as stationary phases for CEC. The amine‐bonded silica NPs were tightly adsorbed on the inner wall of a capillary and generated fast electro‐osmotic flow toward the anode in an electric field.…”

Section: Column Techniquementioning

confidence: 99%

Recent advances in microscale separation

et al. 2017

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Recent advances in microscale separationThis review focuses on the recent advances on microscale separation technology, particularly on the new development on column and detection technologies in electrophoretic and chromatographic separation modes (i.e., CE, CEC, cLC, etc.). Representative examples illustrate also applications on the small molecules, peptides, proteins, polysaccharides, chiral and so on. 246 relevant articles are cited covering the literature published

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“…The simplicity of functionalizing silica nanoparticles ensures their broad application in this area of analysis. Surface‐functionalized silica nanoparticles have been successfully used for the separation of charged and neutral compounds , glucose oligomer derivatives and nucleic acid bases , and cyclodextrin functionalization has been used for chiral separation .…”

Section: Improvement Of Ce Separationmentioning

confidence: 99%

CE and nanomaterials – Part II: Nanomaterials in CE

Adam

Vaculovičová

2017

Electrophoresis

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The scope of this two-part review is to summarize publications dealing with CE and nanomaterials together. This topic can be viewed from two broad perspectives, and this article is trying to highlight these two approaches: (i) CE of nanomaterials, and (ii) nanomaterials in CE. The second part aims at summarization of publications dealing with application of nanomaterials for enhancement of CE performance either in terms of increasing the separation resolution or for improvement of the detection. To increase the resolution, nanomaterials are employed as either surface modification of the capillary wall forming open tubular column or as additives to the separation electrolyte resulting in a pseudostationary phase. Moreover, nanomaterials have proven to be very beneficial for increasing also the sensitivity of detection employed in CE or even they enable the detection (e.g., fluorescent tags of nonfluorescent molecules).

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Capillary electrochromatography using monoamine- and triamine-bonded silica nanoparticles as pseudostationary phases

Cited by 16 publications

References 28 publications

Recent developments in capillary and microchip electroseparations of peptides (2015–mid 2017)

Recent developments in capillary and microchip electroseparations of peptides (2015–mid 2017)

Recent advances in microscale separation

CE and nanomaterials – Part II: Nanomaterials in CE

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