Capillary electrophoresis and small molecule drug discovery: a perfect match?

Espada, Alfonso; Molina-Martin, Manuel

doi:10.1016/j.drudis.2012.02.008

Cited by 31 publications

(25 citation statements)

References 60 publications

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“…To find the suitable conditions for the chiral separation of the model molecules, a series of preliminary experiments were conducted with different buffer compositions at different pH values, in order to characterize the electrophoretic behavior of the analytes in an achiral system over a wide adjustable pH range (2)(3)(4)(5)(6)(7)(8)(9)(10)(11). In the preliminary analysis we used: 25 mM phosphoric acid (pH -2.1), 25 mM sodium didydrogenophosphate (pH -5.0), 25 mM disodium hydrogenophosphate -sodium didydrogenophosphate (1:1) (pH -7.0) and 25 mM sodium tetraborate (pH -9.3) BGEs respectively and we modified the pH of the buffer by adding a 0.1M sodium hydroxide solution.…”

Section: Resultsmentioning

confidence: 99%

Captisol® as Chiral Selector in Capillary Electrophoresis of Non-Acidic Drugs

Budău

Hancu

Szabó

et al. 2017

J. Chil. Chem. Soc.

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Enantioseparation of nine extensively used chiral pharmaceutical substances from different pharmacological classes and with different structural characteristics has been investigated by capillary zone electrophoresis using Captisol® (sulfobuthylether-β-cyclodextrin sodium salt) as chiral selector. The influence on the chiral separation of several parameters including pH and concentration of the background electrolyte, chiral selector concentration, applied voltage, system temperature and injection parameters were studied and optimized in order to obtain increased chiral resolution and shorten analysis time. The results were compared with those obtained with native and derivatized neutral cyclodextrins (β-cyclodextrin and hydroxypropryl-β-cyclodextrin). All model molecules were resolved using Captisol® as chiral additive and optimized electrophoretic conditions, proving the efficiency of this anionic derivative of β-cyclodextrin as chiral selector in capillary electrophoresis.

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Section: Resultsmentioning

confidence: 99%

Captisol® as Chiral Selector in Capillary Electrophoresis of Non-Acidic Drugs

Budău

Hancu

Szabó

et al. 2017

J. Chil. Chem. Soc.

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“…To meet the increasing demand for HTS of kinase activity modulators, assay technologies have evolved rapidly in recent years. Efficient and comprehensive screening of enzyme activity with suitable sample throughput was accomplished by using capillary array electrophoresis as well as multichannel microfluidic electrophoresis coupled with multiplexed optical (UV, LIF), conductivity, and MS detectors . The goal is to decrease analysis time particularly by performing parallel assays.…”

Section: High‐throughput and Multiplex Approachesmentioning

confidence: 99%

“…. Other applications can be found in different remarkable reviews on CE enzyme assays in general and on EMMA or TDLFP specifically .…”

Section: Introductionmentioning

confidence: 99%

Advances in capillary electrophoresis for miniaturizing assays on kinase enzymes for drug discovery

Nehmé

Morin

2015

Electrophoresis

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CE has become a frequently used tool for miniaturizing enzyme assays due particularly to its well-recognized low sample consumption. CE-based enzyme assays cover all aspects of kinetic analysis including the evaluation of enzyme activity, substrate and modulator characterization, and identification. These assays are performed to conduct high-quality primary (hit finding) and secondary (confirmation by determining the half maximal inhibitory concentration, IC ) screening. Nowadays, the kinase family is among the most actively studied pharmaceutical targets in oncology, and in neurodegenerative and inflammation diseases. In this article, we review the fundamentals of the different approaches that may be employed for assaying kinase kinetics. Their advantages and limitations will also be discussed by covering the literature of CE-based assays for purified kinases as well as for kinases in living cells. The last section will be devoted to perspectives by showing some applications of multiplexed and of microchip devices for high-throughput screening kinase assays.

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“…These analyses have been summarized elsewhere, for example, in [25][26][27][28] and are not included in the present review either. For earlier compilations of the use of capillary electromigration methods in drug analysis see, for example, [7,[10][11][12][29][30][31][32][33][34].…”

Section: Introductionmentioning

confidence: 99%

Recent advances in capillary electrophoretic migration techniques for pharmaceutical analysis

et al. 2014

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Since the introduction about 30 years ago, CE techniques have gained a significant impact in pharmaceutical analysis. The present review covers recent advances and applications of CE for the analysis of pharmaceuticals. Both small molecules and biomolecules such as proteins are considered. The applications range from the determination of drug-related substances to the analysis of counterions and the determination of physicochemical parameters. Furthermore, general considerations of CE methods in pharmaceutical analysis are described.

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Capillary electrophoresis and small molecule drug discovery: a perfect match?

Cited by 31 publications

References 60 publications

Captisol® as Chiral Selector in Capillary Electrophoresis of Non-Acidic Drugs

Captisol® as Chiral Selector in Capillary Electrophoresis of Non-Acidic Drugs

Advances in capillary electrophoresis for miniaturizing assays on kinase enzymes for drug discovery

Recent advances in capillary electrophoretic migration techniques for pharmaceutical analysis

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