Capillary electrophoresis as a sample separation step to mass spectrometry analysis: A primer

Lapizco‐Encinas, Blanca H.; Zhang, Y. Victoria; Gqamana, Putuma P.; Křenková, Jana; Foret, František

doi:10.1016/j.trac.2023.117093

Cited by 7 publications

(2 citation statements)

References 70 publications

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“…Determination by CZE coupled with MS detection CZE-MS is a very useful method not only for analysis but also for characterization of (bio)molecules via the determination of their relative molecular masses and elucidation of their structure from their fragmentation patterns [88]. Biopharmaceuticals may contain multiple species of various sizes, such as product-related aggregates (dimers, tetramers), and fragments of low molecular mass relative to the parent molecule.…”

Section: 62mentioning

confidence: 99%

Determination of physicochemical parameters of (bio)molecules and (bio)particles by capillary electromigration methods

Štěpánová,

Kašička

2024

J of Separation Science

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The review provides an overview of recent developments and applications of capillary electromigration (CE) methods for the determination of important physicochemical parameters of various (bio)molecules and (bio)particles. These parameters include actual and limiting (absolute) ionic mobilities, effective electrophoretic mobilities, effective charges, isoelectric points, electrokinetic potentials, hydrodynamic radii, diffusion coefficients, relative molecular masses, acidity (ionization) constants, binding constants and stoichiometry of (bio)molecular complexes, changes of Gibbs free energy, enthalpy and entropy and rate constants of chemical reactions and interactions, retention factors and partition and distribution coefficients. For the determination of these parameters, the following CE methods are employed: zone electrophoresis in a free solution or in sieving media, isotachophoresis, isoelectric focusing, affinity electrophoresis, electrokinetic chromatography, and electrochromatography. In the individual sections, the procedures for the determination of the above parameters by the particular CE methods are described.

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Section: 62mentioning

confidence: 99%

Determination of physicochemical parameters of (bio)molecules and (bio)particles by capillary electromigration methods

Štěpánová,

Kašička

2024

J of Separation Science

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“…The recent developments in the area of MS detection in CE methods have been described in several reviews dealing with CE-MS in general [195][196][197][198] and with its application to peptide, protein, peptidomic, proteomic, and metabolomic analyses especially [8,16,44,[199][200][201].…”

Section: Mass Spectrometrymentioning

confidence: 99%

Recent developments in capillary and microchip electroseparations of peptides (2021–mid‐2023)

Kašička

2023

Electrophoresis

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This review article brings a comprehensive survey of developments and applications of high‐performance capillary and microchip electromigration methods (zone electrophoresis in a free solution or in sieving media, isotachophoresis, isoelectric focusing, affinity electrophoresis, electrokinetic chromatography, and electrochromatography) for analysis, micropreparation, and physicochemical characterization of peptides in the period from 2021 up to ca. the middle of 2023. Progress in the study of electromigration properties of peptides and various aspects of their analysis, such as sample preparation, adsorption suppression, electroosmotic flow regulation, and detection, are presented. New developments in the particular capillary electromigration methods are demonstrated, and several types of their applications are reported. They cover qualitative and quantitative analysis of synthetic or isolated peptides and determination of peptides in complex biomatrices, peptide profiling of biofluids and tissues, and monitoring of chemical and enzymatic reactions and physicochemical changes of peptides. They include also amino acid and sequence analysis of peptides, peptide mapping of proteins, separation of stereoisomers of peptides, and their chiral analyses. In addition, micropreparative separations and physicochemical characterization of peptides and their interactions with other (bio)molecules by the above CE methods are described.

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A Comprehensive Review on Capillary Electrophoresis–Mass Spectrometry in Advancing Biomolecular Research

Pont,

Vergara‐Barberán,

Carrasco‐Correa

2024

Electrophoresis

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This review provides an in‐depth exploration of capillary electrophoresis–mass spectrometry (CE–MS) in biomolecular research from 2020 to 2024. CE–MS emerges as a versatile and powerful tool due to its numerous advantages, facilitating the analysis of various biomolecules, including proteins, peptides, oligonucleotides, and other metabolites, such as lipids, carbohydrates, or amines, among others. The review extends to various CE modes and interfaces for the CE–MS coupling, offering comprehensive insights into their applications within biomolecular research. Furthermore, it effectively summarizes the conditions employed in CE–MS while also addressing critical aspects such as sample preparation requirements. Despite its advantages, the review highlights a gap between discovery and practical implementation, underscoring the need for large‐scale validation and method standardization to fully realize the potential of CE–MS in biomolecular research.

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Capillary electrophoresis as a sample separation step to mass spectrometry analysis: A primer

Cited by 7 publications

References 70 publications

Determination of physicochemical parameters of (bio)molecules and (bio)particles by capillary electromigration methods

Determination of physicochemical parameters of (bio)molecules and (bio)particles by capillary electromigration methods

Recent developments in capillary and microchip electroseparations of peptides (2021–mid‐2023)

A Comprehensive Review on Capillary Electrophoresis–Mass Spectrometry in Advancing Biomolecular Research

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