Capillary electrophoresis as a screening tool for alpha amylase inhibitors in plant extracts

Hamdan, Imad I.; Afifi, Fatima

doi:10.1016/j.jsps.2010.02.004

Cited by 9 publications

(1 citation statement)

References 21 publications

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“…In previous reports, potential α-amylase inhibitor producing microorganisms were screened using the iodine-starch colorimetry method or 3, 5-dinitrosalicylic acid (DNS, Bernfeld method [16]), and recently electrophoresis methods [17]. The iodine-starch colorimetric method is semi-quantitative, time-consuming, and the broad inflection point often leads to inaccuracies [18].…”

Section: Introductionmentioning

confidence: 99%

A new microtiter plate-based screening method for microorganisms producing Alpha-amylase inhibitors

Feng

Wang

Zheng

2011

Biotechnol Bioproc E

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Alpha-amylase inhibitors are widely used by the pharmaceutical and agricultural industries, such as the treatment of diabetes and obesity and insect controller. Here, we developed a colorimetric method to screen for α-amylase inhibitor producing strains or mutants with higher α-amylase inhibitor productivity. This method relies on absorbance changes at 402 nm that are due to the inhibition of α-amylase catalyzed hydrolysis of 2-Chloro-4-nitrophenyl-4-O-β-D-galactopyranosyl-maltoside by α-amylase inhibitors. The assay can be performed on a microtiter plate, making it simple and convenient. Using this method, α-amylase inhibitor producing strains and mutants with higher α-amylase inhibitor productivity can be rapidly screened. One strain, ZJB-08196, with the highest α-amylase inhibition was isolated and identified as Actinoplanes utahensis, and one mutant with higher acarbose production was obtained by screening 3,000 variants using this method.

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Section: Introductionmentioning

confidence: 99%

A new microtiter plate-based screening method for microorganisms producing Alpha-amylase inhibitors

Feng

Wang

Zheng

2011

Biotechnol Bioproc E

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α-Amylase Inhibitor’s Performance in the Control of Diabetes Mellitus: An Application of Computational Biology

Verma

Awasthi

Jamal

et al. 2018

Current Trends in Bioinformatics: An Insight

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On-line immobilized acetylcholinesterase microreactor for screening of inhibitors from natural extracts by capillary electrophoresis

et al. 2012

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In this study we developed a simple capillary electrophoresis (CE) method with an on-line acetylcholinesterase (AChE) microreactor at the inlet of capillary for inhibitor screening. The fused-silica capillary surface was modified with a polycationic polyethylenimine coating. Solutions of the enzyme and chitosan were then injected to immobilize the enzyme in approximately 2.9 cm of the capillary inlet (total length of capillary 60.2 cm) by electrostatic interaction and the film overlay technique. Separation of enzyme reaction product (thiocholine, ThCh) and unreacted substrate (acetylthiocholine, AThCh) was achieved within 3.0 min. The conditions affecting the efficiency of reaction of the enzyme were optimized by measuring the peak area of ThCh. Under the optimum conditions, using Huperzine-A as model inhibitor, K (i) and IC (50) were 0.551 μmol L(-1) and 1.52 μmol L(-1), respectively, for immobilized AChE. Finally, screening of a small compound library containing two known AChE inhibitors and 30 natural extracts was conducted, and species with inhibition activity were directly identified. Compared with previous publications on screening for AChE inhibitors in natural products based on CE methods, the method developed in this work has the advantages of lower cost per analysis, less leakage, and better bioaffinity for the immobilized enzyme because of the unique properties of sodium alginate and chitosan.

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Capillary electrophoresis as a screening tool for alpha amylase inhibitors in plant extracts

Cited by 9 publications

References 21 publications

A new microtiter plate-based screening method for microorganisms producing Alpha-amylase inhibitors

A new microtiter plate-based screening method for microorganisms producing Alpha-amylase inhibitors

α-Amylase Inhibitor’s Performance in the Control of Diabetes Mellitus: An Application of Computational Biology

On-line immobilized acetylcholinesterase microreactor for screening of inhibitors from natural extracts by capillary electrophoresis

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