Capillary electrophoresis as a versatile tool for the bioanalysis of drugs—a review

Boone, C.M; Waterval, Joop C. M.; Lingeman, H.; Ensing, K; Underberg, W.J.M.

doi:10.1016/s0731-7085(99)00088-6

Cited by 77 publications

(52 citation statements)

References 220 publications

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“…CE applied to the drug analysis in complex biological matrices has replaced HPLC in many cases. There are dozens of recent reviews and hundreds of articles that focus on the CE pharmaceutical and biological applications, some with emphasis in the method development [1,[64][65][66]. Nishi reviews the growth of CE application in the analysis of pharmaceuticals: the main components, natural medicines, besides the measurement of physicochemical properties of drugs [1].…”

Section: Pharmaceutical Clinical and Forensic Applicationsmentioning

confidence: 99%

“…Nishi reviews the growth of CE application in the analysis of pharmaceuticals: the main components, natural medicines, besides the measurement of physicochemical properties of drugs [1]. Boon et al present a review of more than 200 bioanalysis of drugs by CE, at this moment it must be larger than the number of drugs analyzed [65]. De Lorenzi reviews the strategies for the analysis of drugs and metabolites in body fluids such as capillary methods of sample concentration and single-step analyses with direct injection of body fluids on-column, and the different modes of CE applied to the drug analysis [66].…”

Section: Pharmaceutical Clinical and Forensic Applicationsmentioning

confidence: 99%

“…5-Fluorouracil, an antineoplasic drug was monitored in tumor and subcutaneous adipose tissue of patients with breast cancer who received the chemotherapy [100]. For more specific information about CE and drug analysis, look for recent reviews [1,3,4,65].…”

Section: Pharmaceutical Clinical and Forensic Applicationsmentioning

confidence: 99%

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Biomedical applications of capillary electrophoresis with laser‐induced fluorescence detection

Páez

Hernández

2001

Biopharm & Drug Disp

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Capillary electrophoresis (CE) is a high-efficiency analytical technique that has had a great impact as a tool in biomedical research, clinical and forensic practice in the last ten years. Only in one of the applications, the DNA analysis, it has had an explosive exponential growth in the last few years. This impact is expressed in an enormous amount of CE articles and many reviews. The CE advantages with respect to other analytical techniques: the required very small sample volume, rapid analysis, great resolution power and low costs, have made this technique ideal for the analysis of a numerous endogenous and exogenous substances present in biological fluids. The different modes of CE have been coupled to different detection techniques such as UV-absorbance, electrochemical, mass spectrometry and laser-induced fluorescence detection (LIFD) to detect different nature and molecular size separated analytes. This review focuses mostly on the applications of CE-LIFD, to measure drugs and endogenous neuroactive substances such as amino acids and monoamines, especially in microdialysis samples from experimental animals and humans. CE-LIFD trends are discussed: automated faster analysis with capillary array systems, resolution power improvement, higher detection sensitivity, and CE systems miniaturization for extremely small sample volume, in order to make CE easier and affordable to the lab bench or the clinical bed.

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Section: Pharmaceutical Clinical and Forensic Applicationsmentioning

confidence: 99%

Section: Pharmaceutical Clinical and Forensic Applicationsmentioning

confidence: 99%

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Biomedical applications of capillary electrophoresis with laser‐induced fluorescence detection

Páez

Hernández

2001

Biopharm & Drug Disp

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“…14,15 On the other hand, electrochemiluminescence (ECL) was applied to a variety of fields such as biosensors, immunoassays, and flow injection analysis, due to its simplicity, inherent sensitivity, low background noise, good selectivity, and wide linear range. 16,17 ECL using a tris(2,2′-bipyridyl)-ruthenium(II) (Ru(bpy)3…”

Section: Introductionmentioning

confidence: 99%

Pharmacokinetics of Propranolol Hydrochlorid in Human Urine by Capillary Electrophoresis Coupled with Electrochemiluminescence

et al. 2011

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“…[10][11][12][13] . All CE modes employ the same instrumentation; samples can be easily analyzed subsequently as diff erencing separation mechanisms.…”

Section: Introductionmentioning

confidence: 99%

Capillary Electrophoresis as a Verification Tool for Immunochemical Drug Screening

Znaleziona¹,

Petr²,

Maier³

et al. 2007

Biomed Pap Med Fac Univ Palacky Olomouc Czech Repub

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Background:The aim of this work was to develop a simple capillary electrophoretic method as the verifi cation and confi rmation tool in the screening analysis for amphetamines, opiates, benzodiazepines and cocaine and their metabolites for toxicological applications.Methods: 50 mM phosphate Tris pH 2.0 with 30 % (v/v) of methanol was used as a background electrolyte that enabled fast separation of drugs and their metabolites in saliva and urine. Verifi cation of the data from the electrophoretic method was done by High Performance Thin Layer Chromatography (HPTLC) and the immunochemical screening test QuikScreen.Results: The experimental conditions of the Capillary Electrophoresis (CE) were partially optimized (mainly the infl uence of concentration and types of additives, e.g. cyclodextrines, organic solvents) and validated; the method was used for analysing samples from drug abusers.Conclusions: The non-instrumental, immunoassay tests could only confi rm qualitative addictions and are mainly employed when the emergency detection of drugs is needed. For quantitative analysis and verifi cation of obtained results the confi rmation step is strongly recommended. The simple screening capillary zone electrophoresis method allows recognition of the most abused drugs. The agreement of the results from CE, HPTLC and QuikScreen test was more than 95 %.

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Capillary electrophoresis as a versatile tool for the bioanalysis of drugs—a review

Cited by 77 publications

References 220 publications

Biomedical applications of capillary electrophoresis with laser‐induced fluorescence detection

Biomedical applications of capillary electrophoresis with laser‐induced fluorescence detection

Pharmacokinetics of Propranolol Hydrochlorid in Human Urine by Capillary Electrophoresis Coupled with Electrochemiluminescence

Capillary Electrophoresis as a Verification Tool for Immunochemical Drug Screening

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