Capillary electrophoresis fingerprints combined with chemometric methods to evaluate the quality consistency and predict the antioxidant activity of Yinqiaojiedu tablet

Abstract

The quality consistency of Yinqiaojiedu tablets (YQJDTs) was monitored by extracting their electrophoretic fingerprint and marker compound data that were obtained using capillary electrophoresis. To select the suitable background electrolyte, wing-shape method was proposed. A background electrolyte composed of 103.1 mM boric acid, 51.6 mM sodium borate, 9.8 mM disodium hydrogen phosphate, and 15.6 mM sodium dihydrogen phosphate was used to separate compounds. Under the optimized conditions, the content of three marker compounds was determined in 25 YQJDT samples. The capillary electrophoresis fingerprints were developed simultaneously, then 25 samples from two manufacturers were clearly divided into two clusters based on the principal component analysis. In fingerprint assessments, systematic quantified fingerprint method was adopted for integrative quality discrimination of YQJDTs from both qualitative and quantitative perspectives, by which the qualities of 25 samples were well differentiated. In addition, partial least squares model was established to explore fingerprint-efficacy relationship between the fingerprints and the antioxidant activities in vitro, providing clinically useful information for quality control. The proposed method was reliable and comprehensive, which could be used for a valuable reference to monitor the quality consistency of traditional Chinese medicines.

Advances in Fingerprint Analysis for Standardization and Quality Control of Herbal Medicines

“…CE has been used for fingerprint analyses of various raw materials and products containing medicinal plants such as Ginkgo biloba ( Johnson and Lunte, 2016 ), Smilax glabra Roxb. ( Zhang and Cheung, 2011 ), Carthamus tinctorius L.( Sun et al, 2003 ), Scutellaria baicalensis ( Yu et al, 2007 ; Wang Y. et al, 2014 ), Dendrobium candidum ( Zha et al, 2009 ), Glycyrrhiza uralensis ( Ma et al, 2017 ; Gong et al, 2021 ), etc. Gong et al recently reported a quality evaluation of Compound Liquorice Tablets (a Western-Chinese herbal preparation containing liquorice extract, powdered poppy, camphor, star anise oil, and sodium benzoate) using CE with UV detection ( Gong et al, 2021 ).…”

Recent Applications of Capillary Electrophoresis in the Determination of Active Compounds in Medicinal Plants and Pharmaceutical Formulations

“…rhamnosyl kaempfero 20 mM phosphate 5 mM β-cyclodextrin (β-CD), 40 [103] nd-no data, LOQ-limit of quantification,…”

Capillary electrophoresis in phytochemical analysis (2014–2017)

“…The 25 samples from two manufacturers were clearly divided into two clusters based on the principal component analysis. In fingerprint assessments, systematic quantified fingerprint method was used for integrative quality discrimination of YQJDTs from both qualitative and quantitative perspectives, by which the qualities of 25 samples were well differentiated .…”