Capillary electrophoresis-immobilized enzyme microreactors for acetylcholinesterase assay with surface modification by highly-homogeneous microporous layer

Liu, Xin; Azhar, Irfan; Khan, Hizbullah; Qu, Qishu; Tian, Miaomiao; Yang, Li

doi:10.1016/j.chroma.2019.460454

Cited by 19 publications

(16 citation statements)

References 51 publications

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“…Traditional open-tubular-based IMERs have a comparatively smaller enzyme–substrate contact area than other IMERs. To overcome this, a method was developed to produce a porous layer modified open-tubular IMER to increase the surface area and the enzyme loading . To produce the porous layer, a biphasic sol–gel was used and enzyme immobilization was performed via ionic binding to a positively charged coating on the capillary surface.…”

Section: Proteinsmentioning

confidence: 99%

“…In the period under review, inhibitors of enzymes in free solution have been characterized with UV detection for ecto-5-nucleotidase, thrombin, histone deacetylase, glucosamine-6 phosphate synthase, and human acetyl-CoA carboxylase 2 and with laser-induced fluorescence for d -amino acid oxidase and BCR-ABL . Further, inhibition studies have been done for immobilized enzymes including acetylcholinesterase, , alanine aminotransferase, β-glucosidase, and sulfotransferase 1A1 using UV detection.…”

Section: Proteinsmentioning

confidence: 99%

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Challenging Bioanalyses with Capillary Electrophoresis

et al. 2019

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This report covers advances in capillary electrophoresis (CE) from January 2018 through September 2019. A summary of the literature during this time period is insightful. A search performed using the SciFinder Scholar® database for journal reports (limited to English) using the term capillary electrophoresis returned approximately 1,800 publications. Further analysis of this list, depicted in Figure 1, provided a snapshot of activity in biomolecular research. Classes of biomolecules most frequently associated with CE publications were proteins, drugs, DNA and metabolites. Another measure of the impact of CE is the translation of this technology into society. A search of patent activity illustrating this process of CE technology transfer returned 346 patents published in all languages, with a substantial contribution reported only in Chinese (198 patents) or English (98 patents). The versatility of CE for biological systems is exemplified by the rise of the technique in several areas. Metabolomics research involving measurements of large sets of molecules with subtle structural differences benefits from rapid separations achieved with high peak capacity and automated instruments. Single cell and sub-cellular analyses continue to progress in CE because of the size compatibility of the technique with the sample. Other examples of areas utilizing CE that are accelerating include portable and printable instrumentation, affinity interaction, as well as proteomics. As an established analytical tool, CE instrumentation and methods have been designed to be accessible and easily adopted by researchers with expertise in areas beyond the field of separations. Generally, publications including CE measurements either outline innovations in the technique or they are compelling applications of a mature analytical approach. The goal of this review, which is limited to 180 citations, is

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Section: Proteinsmentioning

confidence: 99%

Section: Proteinsmentioning

confidence: 99%

Challenging Bioanalyses with Capillary Electrophoresis

et al. 2019

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“…In comparison with a bare‐capillary open‐tubular IMER (without the porous layer), the enzyme was more stable in successive assays. Enzyme activity and inhibition studies were performed, and as an application, the concentration of the AChE inhibitor paraoxon was determined in three types of vegetable juices, each analytical run taking 3 min [78].…”

Section: Heterogeneous Enzyme Reaction Studiesmentioning

confidence: 99%

“…Figure 5. Preparation process for 3D porous layer open-tubular IMERs and application for enzyme assays and inhibition study of AChE.Reproduced with permission from[78].…”

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confidence: 99%

Overview of in‐capillary enzymatic reactions using capillary electrophoresis

2021

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This review summarizes the research that has recently been performed on in‐capillary enzymatic reactions integrated with capillary electrophoresis. The manuscript is subdivided in homogeneous and heterogeneous approaches. The main homogeneous techniques are Electrophoretically Mediated Microanalysis, At‐inlet and Transverse Diffusion of Laminar Flow Profiles. The main heterogeneous ones are Immobilized MicroEnzyme Reactors with enzymes grafted on either non‐magnetic or magnetic particles. The overview covers the period from 2018 to early 2021. The applications range from drug discovery over natural products to food, beverage and pesticide analysis.

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“…The process for coating the inner wall of the fused-silica capillary using a hydrothermal sol-gel method was previously developed by our group [25], based on previous reported methods [26][27][28]. The sol-gel mixture consists of cyclohexane (3.00 mL), TEOS (500 µL), ultrapure water (3.00 mL), CTAB (100 mg), urea (60.0 mg), 0.10 mM acetic acid (440 µL), and n-pentanol (92 µL).…”

Section: Preparation Of Silica-layer Coated Capillary By Hydrothermal Sol-gel Processmentioning

confidence: 99%

Capillary electrophoresis-UV analysis using silica-layer coated capillary for separation of seven phenolic acids and caffeine and its application to tea analysis

et al. 2021

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This work presents an innovative silica-layer coated capillary with comparison study of the silica-layer coated capillary and the fused-silica capillary for the separation of seven phenolic acids viz. p-hydroxyphenylacetic acid (PHPA), p-coumaric acid (PCA), p-hydroxybenzoic acid (PHBA), caffeic acid (CFA), (3,4-dihydroxyphenyl)acetic acid (DHPA), gallic acid (GLA), and 2,3,4-trihydroxybenzoic acid (THBA), together with caffeine (CF), by capillary electro-chromatography (CEC) and micellar electrokinetic chromatography (MEKC), respectively. The running buffer was 25.0 mM borate at pH 9.0, with addition of 50.0 mM sodium dodecyl sulfate for the MEKC mode. The non-coated capillary could not separate all seven phenolic acids by CEC or MEKC. This was achieved using the coated capillary for both CEC and MEKC. The innovative coated capillary with CEC had plate number N ≥ 2.0 × 104 m−1 and resolution Rs ≥ 1.6 for all adjacent pairs of peaks. The capillary was also able to separate GLA and THBA which are structural isomers. Although MEKC mode provided comparable efficiency and selectivity, the reduced EOF of the coated capillary led to longer separation time. The linear calibration range of the seven phenolic acids and caffeine were different but the coefficients of determinations (r2) were all > 0.9965. The precisions of the relative migration times and peak area ratios of analyte to internal standard were 0.1–1.8% and 1.8–6.8%, respectively. There were no statistical differences in the efficiency of separation of the phenolic acids and caffeine for three coated capillaries. It was applied to the analysis of caffeine and phenolic acids in brewed tea using tyramine as the internal standard. The tea samples were diluted prior to analysis by CEC. The separation was less than 15 min. Caffeine, gallic acid and p-coumaric acid were detected and quantified. Caffeine and gallic acid contents were 10.8–15.0 and 2.6–4.8 mg g−1 dry tea leaves, respectively. p-Coumaric acid was detected in only one of the samples with a content of 0.4 mg g−1. Percent recoveries of spiked diluted samples were 90 ± 9 to 106 ± 13%, respectively. Article highlights Silica-layer coated capillary is first reported for simultaneous separation of seven phenolic acids by non-MEKC analysis. Performance between coated, and non-coated capillaries with analysis by CEC and MEKC were compared. Plate number, resolution, capillary reproducibility, and electroosmotic flow mobility are investigated. Graphical abstract

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Capillary electrophoresis-immobilized enzyme microreactors for acetylcholinesterase assay with surface modification by highly-homogeneous microporous layer

Cited by 19 publications

References 51 publications

Challenging Bioanalyses with Capillary Electrophoresis

Challenging Bioanalyses with Capillary Electrophoresis

Overview of in‐capillary enzymatic reactions using capillary electrophoresis

Capillary electrophoresis-UV analysis using silica-layer coated capillary for separation of seven phenolic acids and caffeine and its application to tea analysis

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