Capillary electrophoresis in the context of drug discovery

Farças, Elena; Pochet, Lionel; Crommen, Jacques; Servais, Anne Catherine; Fillet, Marianne

doi:10.1016/j.jpba.2017.02.022

Cited by 31 publications

(27 citation statements)

References 113 publications

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“…Many complex reaction systems in nature require the participation of at least two different enzymes . However, most open‐tubular CE‐integrated IMERs are limited to the immobilization of single enzyme, which significantly confines their broad practical application.…”

Section: Introductionmentioning

confidence: 99%

Controllable and high‐performance immobilized enzyme reactor: DNA‐directed immobilization of multienzyme in polyamidoamine dendrimer‐functionalized capillaries

Shen

Zhou

et al. 2020

Electrophoresis

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In recent years, CE‐integrated immobilized enzyme reactors (IMERs) for single‐enzyme immobilization have attracted considerable attention. However, there has been little research on multienzyme immobilization in CE. Here, we introduce a method for fabricating a CE‐integrated IMER, using DNA‐directed immobilization to fix glucose oxidase and horseradish peroxidase in the capillary, which had been functionalized with polyamidoamine dendrimer (PAMAM). Owing to the reversibility of DNA hybridization, the reactor is capable of dynamic immobilization. Moreover, by introducing the PAMAM, the loading capacity of the IMER is greatly enhanced, and the PAMAM can spontaneously form complexes with DNA and then contribute to the efficiency and stability of the reactor. After 25 days storage, the prepared IMER ultimately retained approximately 70% of its initial activity. We also used the IMER to detect glucose, and the favorable linearity was obtained over the concentration range of 0.78–12.5 mM, with an LOD of 0.39 mM, demonstrating that the CE‐integrated IMER can be applied to actual samples. We believe that this strategy can be extended to other multienzyme immobilization systems, and CE‐integrated IMERs are potentially useful in a wide range of biochemical research applications.

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Section: Introductionmentioning

confidence: 99%

Controllable and high‐performance immobilized enzyme reactor: DNA‐directed immobilization of multienzyme in polyamidoamine dendrimer‐functionalized capillaries

Shen

Zhou

et al. 2020

Electrophoresis

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“…Drug discovery theme has also been studied with CE applications. Farcaş et al [7] have recently overviewed the use of CE in this context. They confirmed the low use of CE in this field though it has been seen as a very efficient and resolutive separation technique.…”

Section: Table I -Recent Applications In Capillary Electrophoresismentioning

confidence: 99%

Shouldn't we be using more capillary electrophoresis nowadays?

Souza¹,

Aurora-Prado²

2019

Br J Anal Chem

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“…Electrophoresis enables the separation and identification of different chemical species. Commonly used for protein and DNA analysis, electrophoresis is of great importance to the biomedical field, as significant applications include drug discovery and disease diagnosis . Traditional electrophoresis instruments separate analytes as they migrate through an electric field with a velocity that is determined by their electrophoretic mobility .…”

Section: Introductionmentioning

confidence: 99%

Counterflow gradient electrophoresis for focusing and elution

Courtney

Ren

2019

Electrophoresis

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Counterflow gradient electrofocusing uses the bulk flow of a liquid solution to counterbalance the electrophoretic migration of an analyte. When either the bulk velocity or the electrophoretic velocity of the analyte is made to vary across the length of the channel, there exists a unique zero‐velocity point for the analyte. This focusing method enables simultaneous separation and concentration of different analytes. The high resolution and sensitivity achieved are similar to that of isoelectric focusing, which separates analytes based on their isoelectric points, but the key difference is that analytes will instead focus based on their electrophoretic mobility. Dynamically changing the applied voltage or the counterflow rate over time will shift the zero‐velocity point, and therefore allows the focused analytes to pass through a fixed detection point, or elute from the separation channel. Throughout the review, a number of different counterflow gradient techniques will be discussed, along with their recent advancements and potential applications.

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Capillary electrophoresis in the context of drug discovery

Cited by 31 publications

References 113 publications

Controllable and high‐performance immobilized enzyme reactor: DNA‐directed immobilization of multienzyme in polyamidoamine dendrimer‐functionalized capillaries

Controllable and high‐performance immobilized enzyme reactor: DNA‐directed immobilization of multienzyme in polyamidoamine dendrimer‐functionalized capillaries

Shouldn't we be using more capillary electrophoresis nowadays?

Counterflow gradient electrophoresis for focusing and elution

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