Capillary Electrophoretic Assay and Purification of Cylindrospermopsin, a Cyanobacterial Toxin from Aphanizomenon ovalisporum, by Plant Test (Blue-Green Sinapis Test)

Alteration of Cylindrospermopsin Content of <i>Aphanizomenon ovalisporum</i> (Cyanobacteria, Nostocales) due to Step-Down from Combined Nitrogen to Dinitrogen

“…What is more, carcinogenic and allergic activities were suggested recently due to the presence of uracil and sulphinated guanidino moieties [35][36][37]. In addition, [27] demonstrated that CYN interferes with plant metabolism as well. The biosynthesis of CYN is understood [26] and its chemical structure and feeding experiments with stable isotopes suggested the role of polyketide synthase [38].…”

“…CYN was purified as our laboratory described earlier [27] and its electropherogram is shown on Figure 5(C). In contrast to the isolated heterocysts, the cyanotoxin extracts of combined nitrogen grown cells contained CYN (Figure 5(B)).…”

“…3 ml culture samples were centrifuged (6.000 g for 3 min) and the pellets were lyophilized and used for CYN assay [27,28]. For CYN content assay of heterocysts, isolated heterocysts were resuspended in 1 mM Tris-HCl pH 7.6 buffer and sonicated for 2 min in 15 s intervals on ice at full power.…”

“…The rest of sonicated extract was centrifuged (10.000 g, 30 min, 4˚C) and the supernatant assayed for CYN content and a part of it saved for Western blotting. The cyanotoxin content was determined with the help of a slightly modified method of capillary electrophoresis as described earlier by our laboratory [27,28]. A longer capillary (85 cm) was used to obtain better separation of the compounds.…”

The ecotoxicological evaluation of Cylindrospermopsis raciborskii from Lake Balaton (Hungary) employing a battery of bioassays and chemical screening

“…ATX and CYN were identified upon retention times and fragmentation patterns of identified peaks: m/z 166 -ATX; m/z 416 -CYN. Quantification of ATX and CYN was performed against dilutions in water of AN-fumarate (Ascent Scientific Ltd., UK) and CYN provided by Dr. Gábor Vasas who had purified the toxin from the Aphanizomenon ovalisporum (Forti) strain (ILC-164), as previously described (Vasas et al, 2002). In the absence of certified standards for HMAN and deoxy-CYN the identity was checked by comparing the fragment ion spectra with those reported by Furey et al (2003) for HMAN and by Seifert et al (2007) for deoxy-CYN.…”