Capillary electrophoretic determination of inorganic and organic anions using 2,6-pyridinedicarboxylic acid: effect of electrolyte's complexing ability

Soga, Tomoyoshi; Ross, Gordon

doi:10.1016/s0021-9673(96)01055-2

Cited by 111 publications

(58 citation statements)

References 14 publications

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“…Detection limits (S/N = 3) ranging from 0.1 to 0.4 mM were achieved. A good detection limit was shown by the comparison with those of Wu et al [36], Soga and Ross [37] as well as Shamsi and Danielson [32] (Table 2). Figure 5.…”

Section: The Separation Of Geometric Isomerssupporting

confidence: 57%

Capillary electrochromatographic analysis of aliphatic mono‐ and polycarboxylic acids

et al. 2003

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The parameters influencing the electrochromatographic separation of aliphatic organic acids in a capillary column with a wall-coated macrocyclic polyamine have been studied. Indirect detection using chromate, pyromellitate, trimellitate, o-phthalate, benzoate and acetate as background electrolytes has been tested. A complete separation of polyprotic acids could be achieved with pyromellitate buffer (7.5 mM, pH 6.5), and satisfactory results for the simultaneous separation of monoprotic acids and polyprotic acids were found using a capillary column of 70 cm (50 cm effective length)x75 microm inner diameter, electrokinetic injection (-10 kV, 10 s), benzoate buffer (6 mM, pH 4.6), separation voltage of -10 kV, and detection at 220 nm. For the separation of the geometric isomers fumarate and maleate, acetate buffer was found the best choice among the background electrolytes tested. The method so established has been applied to the determination of organic acids in soy sauce, brandy, lemon juice, spinach juice and cigarette. From the retention behavior, it was found that the separation mechanism on the bonded phase was influenced by the macrocyclic effect, electrostatic attraction, hydrogen bonding, van der Waals forces, and anion exchange, in addition to the differences in electrophoretic mobility.

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Section: The Separation Of Geometric Isomerssupporting

confidence: 57%

Capillary electrochromatographic analysis of aliphatic mono‐ and polycarboxylic acids

et al. 2003

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“…The indirect UV detection requires the addition of an appropriate chromophore agent to the BGE. Various chromophores have been used for indirect UV detection of carboxylic acids: naphthalene sulfonates [22,23], 2,6-naphthalenedicarboxylic acid [31], trimethoxybenzoic acid [32], 2,6-pyridinedicarboxylic acid [33,34,35], 3,5-dinitrobenzoic acid [36], 5-sulfocalicylate [37], and chromate [38], commonly used for indirect UV detection of anions in CE.…”

Section: Determination Of Pfcas With Indirect Uv Detectionmentioning

confidence: 99%

Separation of perfluorocarboxylic acids using capillary electrophoresis with UV detection

et al. 2005

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A capillary electrophoretic method with UV detection for separation and quantitation of perfluorocarboxylic acids (PFCAs) from C6-PFCA to C12-PFCA has been developed. The optimization of measurement conditions included the choice of the most appropriate type and concentration of buffer in the background electrolyte (BGE), as well as the type and the content of an organic modifier. The optimal separation of investigated PFCAs was achieved with 50 mM phosphate buffer and 40% isopropanol in the BGE using direct UV detection. The optimum wavelength for direct UV detection was optimized at 190 nm. For indirect detection, several chromophores were studied. Five mM 3,5-Dinitrobenzoic acid (3,5-DNBA) in 20 mM phosphate buffer BGE and indirect UV detection at 280 nm gave the optimal detection and separation performance for the investigated PFCAs. The possibility of on-line preconcentration of solutes by stacking has been examined for indirect detection. The detection limits (LODs) determined for direct UV detection ranged from 2 microg/mL for C6-PFCA to 33 microg/mL for C12-PFCA. The LODs obtained for indirect UV detection were comparable to those obtained for direct UV detection.

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“…Acetic and succinic acid as well as formic acid in case of the kinetic experiments were determined by capillary zone electrophoresis on a HP 3D CE system (Hewlett-Packard) in a quartz capillary (length 72 cm, diameter 75 µm) at -25 kV and 20°C according to a modified method of Soga and Ross [28]. The buffer contained pyridine-2,6-dicarboxylic acid (c = 5 mmol/L), cetyltrimethylammonium bromide (c = 0.5 mmol/L), and was adjusted to pH = 5.6 with sodium hydroxide (c = 1 mol/L).…”

Section: Methodsmentioning

confidence: 99%

Alkaline Degradation of Dissolved Organic Matter

Brinkmann

Abbt‐Braun

Frimmel

2003

Acta hydrochim. hydrobiol.

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The degradation of dissolved organic matter (DOM) was studied in alkaline solution. The products were characterised using UV/vis spectroscopy, size-exclusion chromatography (SEC), and by the analysis of low-molecular-weight organic acids (LMWOA). The degradation experiments were performed with water from a brown water lake or its isolated fulvic acid fraction and sodium hydroxide at different reaction times and temperatures. Depending on the wavelength and the reaction time, the UV/vis absorbance between 230 nm and 600 nm increased or decreased. The behaviour of model compounds during reactions in alkaline media was compared to the UV/vis spectroscopic behaviour of DOM. The release of LMWOA was described by kinetic data and compared to the data of model reactions. Evidence was given for the carboxylic esters playing a significant role in the release of LMWOA only during the beginning of the alkaline degradation.The results gained by SEC with on-line UV and DOC detection showed that the average size of DOM was decreasing, and that a major part of the degradation products consisted of low-molecular-weight mono-and dicarboxylic acids. Alkalischer Abbau von gelöster organischer MaterieDer Abbau von gelöster organischer Materie (DOM) in alkalischer Lösung wurde untersucht. Die Produkte wurden mittels UV/vis-Spektroskopie, Größenausschlusschromatogra-phie (SEC) und durch die Analytik niedermolekularer organischer Säuren charakterisiert (LMWOA). Die Abbauversuche wurden mit Wasser aus einem Braunwassersee oder der isolierten Fulvinsäurefraktion und Natronlauge bei verschiedenen Reaktionszeiten und Temperaturen durchgeführt. Abhängig von der Wellenlänge und der Reaktionszeit nahm die UV/vis-Absorption zwischen 230 nm und 600 nm zu oder ab. Das UV/vis-spektroskopische Verhalten der DOM bei den Reaktionen im alkalischen Medium wurde mit dem von Modellsubstanzen verglichen. Die Freisetzung von LMWOA wurde durch kinetische Daten beschrieben und mit den Daten von Modellreaktionen verglichen. Es ergaben sich Hinweise, dass Carbonsäureester nur zu Beginn des alkalischen Abbaus eine Rolle bei der Freisetzung der LMWOA spielen. Mittels SEC gekoppelt an eine Online-UV-und -DOC-Detektion konnte gezeigt werden, dass die mittlere Größe der DOM abnimmt und dass ein wesentlicher Anteil der Abbauprodukte aus niedermolekularen Mono-und Dicarbonsäuren besteht.

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Capillary electrophoretic determination of inorganic and organic anions using 2,6-pyridinedicarboxylic acid: effect of electrolyte's complexing ability

Cited by 111 publications

References 14 publications

Capillary electrochromatographic analysis of aliphatic mono‐ and polycarboxylic acids

Capillary electrochromatographic analysis of aliphatic mono‐ and polycarboxylic acids

Separation of perfluorocarboxylic acids using capillary electrophoresis with UV detection

Alkaline Degradation of Dissolved Organic Matter

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