Capillary gas chromatography-mass spectrometry of Amaryllidaceae alkaloids

Kreh, Mirko; Matusch, Rudolf; Witte, Ludger

doi:10.1016/0031-9422(94)00725-9

Cited by 83 publications

(84 citation statements)

References 16 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Kreh et al (1995), who applied for the first time GC-MS in the analysis of underivatized Amaryllidaceae alkaloids from Narcissus , 1992). Vitamins and organic components had slight effect on the alkaloids production.…”

Section: Resultsmentioning

confidence: 99%

Gas Chromatography-Mass Spectrometry (GC-MS) Analysis of Indole Alkaloids Isolated from <i>Catharanthus roseus </i> (L.) G. Don Cultivated Conventionally and Derived from In vitro Cultures

Wesołowska

Grzeszczuk

Wilas

et al. 2016

Not Bot Horti Agrobo

View full text Add to dashboard Cite

Catharanthus roseus (periwinkle) is a medicinal plant commonly known for its wide biological activity. In many countries different parts of this plant are used for the treatment of diabetes, hypertension and for menstrual regulation. Due to the ability of production of alkaloids, which can be applied in cancer therapy, is still extensively investigated. Two, the most valuable alkaloids (vincristine and vinblastine) are present in C. roseus in very low concentrations. Micropropagation is promising technique used to enhance the level of important secondary metabolites. The main objective of present study was alkaloids extraction from plants cultivated conventionally and derived from in vitro cultures. In this order the aerial parts of periwinkle were extracted with 96% ethanol at room temperature (method I) and heated with 96% ethanol at 55 °C for 90 minutes (method II). The obtained mixtures of different indole alkaloids were analyzed by gas chromatography -mass spectrometry (GC-MS). Analysis revealed the presence of 15 alkaloids, among which vindoline, vindorosine, isovindolinine and ajmalicine were the most abundant. The obtained results indicated that the propagation method had a significant effect on the percentage content of alkaloids in C. roseus herb. Plants derived from in vitro cultures were richer in vindorosine and vindoline, while conventionally cultivated -in tetrahydroalstonine and ajmalicine. Moreover, in case of isovindolinine, vindolinine and ajmalicine, extraction at 55 °C was more effective, while for pericyclivine -maceration at room temperature. Interestingly, the pericyclivine was not detected in the mixture of alkaloids obtained from periwinkle herb by the extraction at 55 °C.

show abstract

Section: Resultsmentioning

confidence: 99%

Gas Chromatography-Mass Spectrometry (GC-MS) Analysis of Indole Alkaloids Isolated from <i>Catharanthus roseus </i> (L.) G. Don Cultivated Conventionally and Derived from In vitro Cultures

Wesołowska

Grzeszczuk

Wilas

et al. 2016

Not Bot Horti Agrobo

View full text Add to dashboard Cite

show abstract

“…It has been found that Amaryllidaceae alkaloids can be analyzed by GC without derivatization and, with only few exceptions, they show a mass spectral fragmentation pattern very similar to those obtained under direct insertion (Kreh et al, 1995). Several studies of the distribution of galanthamine in different subspecies and populations of mainly Galanthus and Narcissus species have been published (Lopéz et al, 2002;Berkov et al, 2004).…”

Section: Introductionmentioning

confidence: 89%

“…Alkaloids of many different skeleton types can be rapidly identified by GC/EI-MS (Wink et al, 1995;Kreh et al, 1995;Suau et al, 2002). It has been found that Amaryllidaceae alkaloids can be analyzed by GC without derivatization and, with only few exceptions, they show a mass spectral fragmentation pattern very similar to those obtained under direct insertion (Kreh et al, 1995).…”

Section: Introductionmentioning

confidence: 94%

“…The injector temperature was 280 °C. The alkaloids were identified by comparison of their MS with those in the NIST library, with those reported in the literature (Berkov et al, 2008;Gotti et al, 2006;Kreh et al, 1995;López et al, 2002), with commercially available standards (galanthamine, Changsha Organic), and with reference compounds isolated earlier in our laboratory (galanthamine, galanthine, lycoramine, haemanthamine, vittatine, and tazettine).…”

Section: Gc/ms Analysismentioning

confidence: 99%

See 1 more Smart Citation

Analysis of Amaryllidaceae alkaloids from Zephyranthes grandiflora by GC/MS and their cholinesterase activity

Cahlíková¹,

Valterová²,

Macáková³

et al. 2011

Rev. bras. farmacogn.

View full text Add to dashboard Cite

Amaryllidaceae are known as ornamental plants, furthermore some species of this family contain galanthamine, an acetylcholinesterase inhibitor approved for the treatment of Alzheimer´s disease, and other alkaloids with interesting pharmacological activity. The chemical composition of alkaloids from Zephyranthes grandiflora Lindl. was analyzed by GC/MS. Seven known compounds, belonging to five structural types of Amaryllidaceae alkaloids, were identified. The alkaloid extract from the bulbs showed promising cholinesterase inhibitory activities against human blood acetylcholinesterase (HuAChE; IC50 39.2±3.0 μg/mL) and human plasma butyrylcholinesterase (HuBuChE; IC50 356±9.3 μg/mL).

show abstract

“…HPLC, etc., and identified by spectroscopy, mainly 1D and 2D NMR. The GC-MS technique has proved to be very effective for rapid separation and identification of complex mixtures of Amaryllidaceae alkaloids obtained from low mass samples (Kreh et al, 1995). Thus, the assessment of alkaloid distribution at species, populational and individual levels and the detection of new compounds have become much easier and faster (Berkov et al, 2007a(Berkov et al, , 2009c(Berkov et al, , 2011).…”

Section: Distribution Of Alkaloids In the Genus Galanthusmentioning

confidence: 99%