Capillary Zone Electrophoresis for the Analysis of Peptides: Fostering Students’ Problem-Solving and Discovery Learning in an Undergraduate Laboratory Experiment

Albright, Jessica C.; Beussman, Douglas J.

doi:10.1021/acs.jchemed.6b00463

Cited by 5 publications

(3 citation statements)

References 32 publications

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“…Enhanced sensitivity of peptide detection at ultralow UV region below 200 nm has been utilized in CZE‐UV analyses at 195 nm of the cancer therapeutic octapeptide, octreotide , and of tetrapeptide bound to chitosan . In the illustrative CZE separation of four peptides (bradykinin, angiotensin 1, Leu‐enkephalin, and Met‐enkephalin) for educational purposes, the 192 nm detection wavelength was used .…”

Section: Detectionmentioning

confidence: 99%

Recent developments in capillary and microchip electroseparations of peptides (2015–mid 2017)

Kašička

2017

Electrophoresis

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The review brings a comprehensive overview of recent developments and applications of high performance capillary and microchip electroseparation methods (zone electrophoresis, isotachophoresis, isoelectric focusing, affinity electrophoresis, electrokinetic chromatography, and electrochromatography) to analysis, microscale isolation, purification, and physicochemical and biochemical characterization of peptides in the years 2015, 2016, and ca. up to the middle of 2017. Advances in the investigation of electromigration properties of peptides and in the methodology of their analysis (sample preseparation, preconcentration and derivatization, adsorption suppression and EOF control, and detection) are described. New developments in particular CE and CEC methods are presented and several types of their applications to peptide analysis are reported: qualitative and quantitative analysis, determination in complex (bio)matrices, monitoring of chemical and enzymatical reactions and physical changes, amino acid, sequence and chiral analysis, and peptide mapping of proteins. Some micropreparative peptide separations are shown and capabilities of CE and CEC methods to provide important physicochemical characteristics of peptides are demonstrated.

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Section: Detectionmentioning

confidence: 99%

Recent developments in capillary and microchip electroseparations of peptides (2015–mid 2017)

Kašička

2017

Electrophoresis

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“…Based on the findings above, the researcher found that one way that can be used to improve students' mathematical abilities is to use the discovery learning model in the process of learning mathematics. This model can be a tool for teachers to improve students' mathematical undertanding abilities (Albright & Beussman, 2017;Dina et al, 2019;Murat & Leman, 2020). This is because this model will involve students directly in every teaching and learning process both inside and outside the classroom.…”

Section: Resultsmentioning

confidence: 99%

The Effectiveness of Discovery Learning Model to Increas Students' Mathematical Understanding Ability

Sartono,

Zainuddin,

Nisa

et al. 2023

jcp

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Mathematics is one of the mandatory subjects that every student needs to understand. In mathematics, fractions are one of the materials that students consider difficult. Several studies have found that students cannot understand the concept of fractions well. Students' ability to understand mathematics is called mathematical comprehension ability. This ability is a basic ability in mastering mathematics. One model that is considered capable of improving students' mathematical abilities is the discovery learning model. This research aims to reveal the effectiveness of the discovery learning model in improving coastal students' mathematical understanding. This research is quantitative research using a pre-experiment design which was carried out at SDN 111 Buton in the odd semester of the 2022/2023 academic year. Based on the results of data processing and analysis, researchers found that the discovery learning model can improve coastal students' mathematical understanding abilities. These findings are evidence that this learning model can be used to improve coastal students' mathematical understanding abilities. Therefore, this model can be an alternative in improving students' mathematical abilities. Matematika sebagai salah satu mata pelajaran wajib yang perlu dipahami oleh setiap siswa. Dalam matematika, pecahan merupakan salah satu materi yang dianggap sulit oleh siswa. Beberapa penelitian menemukan bahwa siswa tidak dapat memahami konsep pecahan dengan baik. Kemampuan siswa dalam memahami matematika disebut dengan kemampuan pemahaman matematika. Kemampuan ini merupakan kemampuan dasar dalam menguasai matematika. Salah satu model yang dianggap mampu meningkatkan kemampuan matematika siswa adalah model discovery learning. Penelitian ini bertujuan untuk mengungkap keefektifan model discovery learning dalam meningkatkan coastal students' mathematical understanding. Penelitian ini merupakan penelitian kuantitatif yang menggunakan desain pre-experiment yang dilaksanakan di SDN 111 Buton pada semester ganjil tahun ajaran 2022/2023. Berdasarkan hasil pengolahan dan analisis data, peneliti menemukan bahwa model discovery learning dapat meningkatkan kemampuan pemahaman matematis siswa pesisir. Temuan tersebut menjadi bukti bahwa model pembelajaran ini dapat digunakan untuk meningkatkan kemampuan pemahaman matematis siswa pesisir. Oleh karena itu, model ini dapat menjadi salah satu alternatif di dalam meningkatkan kemampuan matematis peserta didik.

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“…Immunochemical assays [14][15][16], capillary zone electrophoresis [17][18][19][20][21], and liquid chromatography [22][23][24][25] are the main methods for the quantitation of peptide-based therapeutics in biological fluids. These methods consider the molecular structure of peptidebased drugs and their physical and chemical properties.…”

Section: Introductionmentioning

confidence: 99%

Development and Validation of an LC-MS/MS Method for Simultaneous Determination of Short Peptide-Based Drugs in Human Blood Plasma

et al. 2022

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A novel HPLC-ESI-MS/MS method for simultaneous gonadotropin-releasing hormone (GnRH) analogs and somatostatin analog quantitation was developed and validated. The developed method was successfully applied to pharmacokinetic studies. The sample preparation process included solid-phase extraction (SPE). Effective chromatographic separation of the analytes and internal standard (dalargin) was achieved with a C18 column, using a gradient elution with two mobile phases: 0.1% v/v formic acid (aqueous solution) and 0.1% v/v formic acid (acetonitrile solution). The linearity of the method was demonstrated within a concentration range of 0.5–20 ng/mL, with correlation coefficients between 0.998–0.999 for goserelin, buserelin, triptorelin, and octreotide, respectively. The relative standard deviation (RSD, %) values for method accuracy and precision did not exceed 20% at the lower level of quantitation (LLOQ) or 15% at other concentration levels.

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Capillary Zone Electrophoresis for the Analysis of Peptides: Fostering Students’ Problem-Solving and Discovery Learning in an Undergraduate Laboratory Experiment

Cited by 5 publications

References 32 publications

Recent developments in capillary and microchip electroseparations of peptides (2015–mid 2017)

Recent developments in capillary and microchip electroseparations of peptides (2015–mid 2017)

The Effectiveness of Discovery Learning Model to Increas Students' Mathematical Understanding Ability

Development and Validation of an LC-MS/MS Method for Simultaneous Determination of Short Peptide-Based Drugs in Human Blood Plasma

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