Capillary zone electrophoretic determination of ionic impurities in silicone products used for electronic applications

Optimization and Validation of a Quantitative Capillary Electrophoresis Sodium Dodecyl Sulfate Method for Quality Control and Stability Monitoring of Monoclonal Antibodies

“…(2) Effect of Incubation Temperature and Time. The effect of temperature (45, 60, 70, and 90 °C) and heating time (5,10,15, and 20 min) on rMAb fragmentation in the absence or presence of the alkylating agent (40 mM IAM) before CE-SDS analysis of nonreduced samples was studied. The pH level of these sample solutions for SDS-rMAb complexation was pH 9.0.…”

“…The emerging technique of capillary electrophoresis (CE) shows many advantages over classical SDS-PAGE including oncolumn direct UV or fluorescence detection, automation, enhanced resolution and reproducibility, and accurate quantification of proteins and molecular weight determination. [7][8][9][10][11][12][13][14] ableand water-soluble linear or slightly branched polymers, such as linear polyacrylamide, poly(ethylene oxide), 15 poly(ethylene glycol), dextran, and pullulan are used as the sieving matrix for CE-SDS. 8,[16][17][18][19] The implementation of these polymer solutions has led to the enhancement of overall precision and robustness of this CE-based methodology.…”

Quantitative capillary zone electrophoresis of inorganic anions

“…As can be seen from the table, the large majority of studies showed no comparison data. For the few that did, the comparison has been mainly between CZE and IC [3,23,25,41,43,45,63,65,67,70,72,74,76,77,84,86,96,97,104,106,110,111,113,119,124,126,127,131,134,135,140,141,146,148,149,152,158,174,190,194,195,219]. Typical examples include studies by Yang et al [126], who found that results obtained using a high-sensitivity method developed using sample stacking technique together with the use of an internal standard correlated well with a standard IC method, and work by Fung and Lau [149], who examined a CZE method for the analysis of inorganic anions in rainwater and used an IC technique in parallel, reporting that the results for the major anions were within statistical variation.…”

“…Accuracy can be achieved in one of two ways, firstly, through direct reference to a known standard, such as a quality control standard or certified reference material, or secondly, through reference to a standard analyzed using an alternative technique that is known to be accurate [218]. 110,111,113,119,124,126,127,131,134,135,140,141,146,148,149,152,158,174,190,194,195,219]. Typical examples include studies by Yang et al [126], who found that results obtained using a high-sensitivity method developed using sample stacking technique together with the use of an internal standard correlated well with a standard IC method, and work by Fung and Lau [149],…”

Protein separation by capillary gel electrophoresis: A review

“…The lifetimes of these columns were limited (usually less than 10 runs) [12], and the run-to-run reproducibility was poor. Currently, replaceable and water-soluble linear or slightly branched polymers, such as linear polyacrylamide [11–13], poly(ethylene glycol) [11], poly(ethylene oxide) [14], dextran [15–17], pullulan [18, 19] and cross-linked polyacrylamide [20–22] are used as sieving matrices for CGE [5, 11, 23–25]. Availability of these polymer matrices has led to improved reproducibility and robustness of this methodology.…”