Capillary zone electrophoretic separation of peptides and proteins using low pH buffers in modified silica capillaries

Selectivity change in the separation of proteins and peptides by capillary electrophoresis using high-molecular-mass polyethyleneimine

“…The use of polymeric coatings has led to high efficiency and reproducible protein separations preventing the surface adsorptions. Polymers ranging from highly hydrophobic to highly hydrophilic were used for this purpose to obtain efficient separations of proteins in CE [1][2][3][4][5][6][7][8][9][10][11][12][13][14][15][16].…”

The influence of surface treatments on the electroosmotic flow in micellar electrokinetic capillary chromatography

“…Also, silanization by trimethylchlorosilane (TMCS) was used to silylate the column surface and reduce the EOF [13,21]. Again, polymer and a number of other coatings were applied by different authors [22][23][24][25][26][27][28] and a decreased EOF was generally observed. Terabe et al [29] investigated the effects on the EOF of hydrophobic and hydrophillic coatings.…”

Separation of ions in acidic solution by capillary electrophoresis

“…The EOF is low up to pH 4.5, rises rapidly between pH 4.5 to 8, and levels off at still higher pH values [5,6]. At about pH 2, the dissociation of the surface silanol groups is completely suppressed, and the value of the zeta potential approaches zero [7].…”