Capn4 mRNA level is correlated with tumour progression and clinical outcome in clear cell renal cell carcinoma

Zhuang, Qianfeng; Qian, Xi; Cao, Yunjie; Fan, Min; Xu, Xianlin; He, Xiaozhou

doi:10.1177/0300060513505524

Cited by 16 publications

(9 citation statements)

References 30 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Understanding the molecular mechanisms of gliomagenesis and tumor progression is necessary to explore more effective diagnostic and therapeutic strategies. In a previous study, it was shown that alterations in Capn4 expression correlated with glioma grade and patient survival (13). In the present study, we have shown that the Capn4 protein levels, but not the mRNA levels, in GBM were higher than the control tissues, which is consistent with our immunohistochemical findings in our previous report (13).…”

Section: Discussionsupporting

confidence: 93%

“…Capn4 overexpression was observed in intrahepatic cholangiocarcinoma tissues and involved in the metastasis of intrahepatic cholangiocarcinoma (12). Higher Capn4 expression was also identified in and associated with poor overall survival (OS) of clear cell renal cell carcinoma patients (13). In a previous study, we also observed an increased expression of Capn4 in glioma tissues, which was associated with a poorer OS and progression-free survival (PFS) of GBM patients.…”

Section: Introductionmentioning

confidence: 67%

See 1 more Smart Citation

miR-124 suppresses the migration and invasion of glioma cells in vitro via Capn4

Cai

Chen

et al. 2015

Oncology Reports

View full text Add to dashboard Cite

miR-124 and Capn4 are aberrantly expressed in glioblastoma multiforme (GBM) tissues. In the present study, we investigated miR-124 and Capn4 expression in GBM tissue specimens. The role of miR-124 and Capn4 in the migration and invasion of glioma cells in vitro was also examined. miR-124 and Capn4 expression in 20 GBM and 6 control brain specimens was examined using RT-qPCR and immuno-blotting. Data from The Cancer Genome Atlas were retrieved. Candidate mRNA target sites of miR-124 were predicted using TargetScan/microRNA and binding was examined using dual luciferase reporter assays. The U87 and U251 cells were transfected with scrambled microRNA, miR-124 mimics and/or pLenti-Capn4 prior to wound‑healing and Transwell invasion assays. Proteins involved in the epithelial-mesenchymal transition were examined using immunoblotting. The results showed that miR-124 was significantly downregulated in GBM tissues. Immunoblotting showed a marked upregulation of Capn4 expression in GBM tissues. The Spearman's correlation analysis revealed a negative association between miR-124 expression and Capn4 protein levels. TargetScan/microRNA predicted the miR-124 binding site in the nucleotide 440-446 region within the Capn4 3'-UTR, which was confirmed by luciferase assays. Wound‑healing and Transwell invasion assays demonstrated that Capn4 downregulation or miR-124 mimics suppressed the migration and invasion of glioma cells. Capn4 downregulation or miR-124 mimics reduced the level of phospho-FAK and MMP2, vimentin and N-cadherin in U87 cells. In conclusion, miR-124 was found to suppress the migration and invasion of glioma cells in vitro via Capn4.

show abstract

Section: Discussionsupporting

confidence: 93%

Section: Introductionmentioning

confidence: 67%

miR-124 suppresses the migration and invasion of glioma cells in vitro via Capn4

Cai

Chen

et al. 2015

Oncology Reports

View full text Add to dashboard Cite

show abstract

“…In tumorigenesis, the prognostic and therapeutic value of calpain-4 have been illustrated in hepatocellular carcinoma, intrahepatic cholangiocarcinoma, clear cell renal cell carcinoma, non-small cell lung cancer, nasopharyngeal carcinoma and glioma [12,13,14,15,16,17]. High calpain-4 expression is found to be positively correlated with tumor invasion and metastasis, and predicts poor prognosis in these types of malignancies.…”

Section: Discussionmentioning

confidence: 99%

“…A recent study indicates that calpain-4 could regulate the organization of the actin cytoskeleton during cell migration [11]. Overexpression of calpain-4 is observed in several types of tumors, such as hepatocellular carcinoma, intrahepatic cholangiocarcinoma, and clear cell renal cell carcinoma, and could be regarded as a predictor of poor prognosis [12,13,14,15,16,17]. Nevertheless, the roles of calpain-4 in gastric carcinoma remain poorly understood.…”

Section: Introductionmentioning

confidence: 99%

Elevated Expression of Calpain-4 Predicts Poor Prognosis in Patients with Gastric Cancer after Gastrectomy

Peng

Min

Song

et al. 2016

IJMS

View full text Add to dashboard Cite

Calpain-4 belongs to the calpain family of calcium-dependent cysteine proteases, and functions as a small regulatory subunit of the calpains. Recent evidence indicates that calpain-4 plays critical roles in tumor migration and invasion. However, the roles of calpain-4 in gastric tumorigenesis remain poorly understood. Herein, we examined calpain-4 expression by immunohistochemical staining on tissue microarrays containing tumor samples of 174 gastric cancer patients between 2004 and 2008 at a single center. The Kaplan-Meier method was used to compare survival curves, and expression levels were correlated to clinicopathological factors and overall survival. Our data demonstrated that calpain-4 was generally increased in gastric cancer cell lines and primary tumor tissues. High expression of calpain-4 was positively associated with vessel invasion, lymph node metastasis, and advanced TNM (Tumor Node Metastasis) stage. Multivariate analysis identified calpain-4 as an independent prognostic factor for poor prognosis. A predictive nomogram integrating calpain-4 expression with other independent prognosticators was constructed, which generated a better prognostic value for overall survival of gastric cancer patients than a TNM staging system. In conclusion, calpain-4 could be regarded as a potential prognosis indicator for clinical outcomes in gastric cancer.

show abstract

“…Calpain small subunit 1 (Capn4) is a small regulatory subunit of the Calpain family and plays a crucial role in the maintenance of calpain’s stability and activity 15. A previous study indicated that the overexpression of Capn4 was the underlying cause of invasion and metastasis after liver transplantation in hepatocellular carcinoma 16. Another study revealed that Capn4 was upregulated and was associated with tumor progression and its clinical outcome in clear cell renal cell carcinoma 17.…”

Section: Introductionmentioning

confidence: 99%

miR-124 suppresses proliferation and invasion of nasopharyngeal carcinoma cells through the Wnt/β-catenin signaling pathway by targeting Capn4

Wang

2017

OTT

View full text Add to dashboard Cite

BackgroundRecent studies have demonstrated that microRNA 124 (miR-124) acts as a tumor suppressor in nasopharyngeal carcinoma (NPC); however, the exact molecular mechanism by which miR-124 exerts tumor suppression has not been well elucidated.Materials and methodsWe performed quantitative real-time PCR (qRT-PCR) to measure the expression of metastasis associated lung adenocarcinoma transcript 1, miR-124, and calpain small subunit 1 (Capn4) mRNAs in NPC cell lines. We also performed western blot analysis to detect the levels of Capn4. Furthermore, we performed MTT assay and transwell invasion assay to determine the proliferation and invasion ability of two NPC cell lines, namely, HONE1 and CNE2 cells, respectively. The verification of targets of miR-124 was performed using prediction softwares and luciferase reporter analysis.ResultsAccording to our results, the expression of Capn4 was found to be elevated, whereas the expression of miR-124 was lowered in NPC cell lines compared with normal nasopharyngeal cells. When we preformed overexpression of miR-124, it suppressed the proliferation and invasion of NPC cells. Moreover, miR-124 suppressed the expression of Capn4 by targeting Capn4 in HONE1 and CNE2 cells. When we preformed overexpression of Capn4, it reversed the inhibitory effect of miR-124 on the proliferation and invasion of NPC cells. Furthermore, miR-124–Capn4 axis decreased the levels of β-catenin, cyclin D1, and c-Myc, the components of the Wnt/β-catenin signaling pathway.ConclusionThe suppression of proliferation and invasion of NPC cells by miR-124 were achieved by the regulation of Wnt/β-catenin signaling pathway by targeting Capn4. The results of this study revealed a novel miR-124–Capn4 regulatory axis in NPC cell lines, providing a better understanding of the pathogenesis of NPC and a promising therapeutic target for patients with NPC.

show abstract

Capn4 mRNA level is correlated with tumour progression and clinical outcome in clear cell renal cell carcinoma

Cited by 16 publications

References 30 publications

miR-124 suppresses the migration and invasion of glioma cells in vitro via Capn4

miR-124 suppresses the migration and invasion of glioma cells in vitro via Capn4

Elevated Expression of Calpain-4 Predicts Poor Prognosis in Patients with Gastric Cancer after Gastrectomy

miR-124 suppresses proliferation and invasion of nasopharyngeal carcinoma cells through the Wnt/β-catenin signaling pathway by targeting Capn4

Contact Info

Product

Resources

About

Capn4 mRNA level is correlated with tumour progression and clinical outcome in clear cell renal cell carcinoma

Cited by 16 publications

References 30 publications

miR-124 suppresses the migration and invasion of glioma cells in vitro via Capn4

miR-124 suppresses the migration and invasion of glioma cells in vitro via Capn4

Elevated Expression of Calpain-4 Predicts Poor Prognosis in Patients with Gastric Cancer after Gastrectomy

miR-124 suppresses proliferation and invasion of nasopharyngeal carcinoma cells through the Wnt/&beta;-catenin signaling pathway by targeting Capn4

Contact Info

Product

Resources

About

miR-124 suppresses proliferation and invasion of nasopharyngeal carcinoma cells through the Wnt/β-catenin signaling pathway by targeting Capn4