Capping and in vivo toxicity studies of gold nanoparticles

Nghiem, Thi Ha Lien; Nguyen, Thi Tuyen; Fort, Emmanuel; Nguyen, Thanh Phuong; Hoang, Thi My Nhung; Nguyen, Thi Thanh Ngan; Tran, Huyen T. T.

doi:10.1088/2043-6262/3/1/015002

Cited by 23 publications

(20 citation statements)

References 21 publications

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“…This study takes the advantages of high Xray absorption of GNPs in spherical shape and their high cellular uptake by breast tumour cells in conjugation with BBN. The uniform GNPs were prepared in the size of 15 ± 2 nm in diameter by general reduction of HAuCl 4 using sodium citrate [20]. It has been shown that the C-terminal amino acid sequence of BBN is necessary for retaining receptor binding affinity and preserving the biological activity of BBN-like peptide [18].…”

Section: Discussionmentioning

confidence: 99%

“…With all newly developed bioconjugate preparations, the potential of toxicity remains an important factor in determining their medical applications [20]. Although GNPs were known to be biocompatible and non‐toxic [10, 13], we performed MTT assay to determine the toxicity of the new bioconjugate in vitro .…”

Section: Discussionmentioning

confidence: 99%

“…UV-vis spectroscopy (Cary 100 Bio, Varian, USA) and Fourier transform infrared spectroscopy (FT-IR) (Nicolet Magna 560 IR, USA) were performed to confirm the binding of GNPs with BBN peptide and its PEGylation [19][20][21].…”

Section: Peg-coated Gnps-bbn Conjugate Characterisationmentioning

confidence: 99%

“…Briefly, T47D cells were planted into a 96-well plate with 200 μl of RPMI medium and incubated at 37 °C under a 5% CO 2 atmosphere for 24 h. The cell plate was rinsed with PBS to remove unattached cells and 1 ml fresh culture medium was added to each well. The medium of each well was then replaced with 100 µl of fresh medium containing various concentrations of PEG-coated GNPs-BBN conjugate (10,20,40,80, and 100 µg/ml). After 24 h, the cells were treated with 10 μl MTT solution (5 mg ml −1 in PBS) and incubated for 4 h. Finally, the wells were evacuated and 100 μl dimethyl sulphoxide was added to each well.…”

Section: Cytotoxicity Studymentioning

confidence: 99%

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BBN conjugated GNPs: a new targeting contrast agent for imaging of breast cancer in radiology

Salouti

Saghatchi

2017

IET nanobiotechnol.

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Using of targeted contrast agents in X-ray imaging of breast cancer can improve the accuracy of diagnosis, staging, and treatment planning by providing early detection and superior definition of tumour volume. This study demonstrates a new class of X-ray contrast agents based on gold nanoparticles (GNPs) and bombesin (BBN) for imaging of breast cancer in radiology. GNPs were synthesised in spherical shape in the size range of 15 ± 2 nm and conjugated with BBN followed by coating with polyethyleneglycol (PEG). The and behaviour of PEG-coated GNPs-BBN conjugate was investigated performing cytotoxicity, binding, and internalisation assays as well as biodistribution and X-ray imaging studies in mouse bearing breast tumour. Cytotoxicity study showed biocompatibility of the prepared bioconjugate. The binding and internalisation studies using T47D cell line approved the targeting ability of new agent. The biodistribution study showed the considerable accumulation of prepared conjugate in breast tumour in mouse model. The breast tumour was clearly visualised in X-ray images taken from the mouse model. The results showed the potential of PEG-coated GNPs-BBN conjugate as a contrast agent in X-ray imaging of breast tumour in humans that need further investigations.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Peg-coated Gnps-bbn Conjugate Characterisationmentioning

confidence: 99%

Section: Cytotoxicity Studymentioning

confidence: 99%

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BBN conjugated GNPs: a new targeting contrast agent for imaging of breast cancer in radiology

Salouti

Saghatchi

2017

IET nanobiotechnol.

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“…Figure 1 presents the schematic of GNS NPs, which were conjugated with different biomolecules. The biofunctionalization process of each biomolecule was performed according to our previous works: with HS-PEG-COOH [4], with GSH [11]; with BSA [13,14], and with anti-HER2 IgG [14]. In brief, a number of above biomolecules was added into the GNS NPs solution to generate biomolecules-coated GNS NPs.…”

Section: Preparation and Biofunctionalization Of Gold Nanoshellsmentioning

confidence: 99%

Biofunctionalization of Gold Nanoshells

Lien¹

2018

JST

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Gold nanoshells were grown on monodispersed and aminopropyltriethoxysilane (APTES) functionalized silica nanoparticles cores with varying sizes ranging from 40-180 nm, which were prepared by Stober route. Gold shells were deposited onto the surface of silica NPs by using tetrakis(hydroxymethyl) phosphonium chloride and electroless gold plating method. The coverage of the gold nanoshells on the surfaces of the silica NPs was evaluated using surface plasmon resonance spectra and scanning electron microscope.

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Surface modification minimizes the toxicity of silver nanoparticles: an in vitro and in vivo study

et al. 2017

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Currently toxicological research in Silver nanoparticle is a leading issue in medical science. The surface chemistry and physical dimensions of silver nanoparticles (Ag-NPs) play an important role in toxicity. The aim of this present study was to evaluate the in vitro and in vivo toxicity of Ag-NPs as well as the alteration of toxicity profile due to surface functionalization (PEG and BSA) and the intracellular signaling pathways involved in nanoparticles mediated oxidative stress and apoptosis in vitro and in vivo system. Ag-NPs released excess Ag ions leads to activation of NADPH oxidase and helps in generating the reactive oxygen species (ROS). Silver nanoparticles elicit the production of excess amount of ROS results activation of TNF-α. Ag-NPs activates caspase-3 and 9 which are the signature of mitochondrial pathway. Ag-NPs are responsible to decrease the antioxidant enzymes and imbalance the oxidative status into the cells but functionalization with BSA and PEG helps to protect the adverse effect of Ag-NPs on the cells. This study suggested that Ag-NPs are toxic to normal cells which directly lead with human health. Surface functionalization may open the gateway for further use of Ag-NPs in different area such as antimicrobial and anticancer therapy, industrial use or in biomedical sciences.

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Capping and in vivo toxicity studies of gold nanoparticles

Cited by 23 publications

References 21 publications

BBN conjugated GNPs: a new targeting contrast agent for imaging of breast cancer in radiology

BBN conjugated GNPs: a new targeting contrast agent for imaging of breast cancer in radiology

Biofunctionalization of Gold Nanoshells

Surface modification minimizes the toxicity of silver nanoparticles: an in vitro and in vivo study

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